Topical application of Zanthoxylum piperitum extract improves lateral canthal rhytides by inhibiting muscle contractions

Abstract Facial wrinkles are the predominant phenotypes of skin aging. To date, one of the most effective ways to improve wrinkles is botulinum toxin type A (BoNT/A) injection, which inhibits muscle contractions by reducing acetylcholine release from neurons. However, since BoNT/A is a hazardous neu...

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Autores principales:	Wooseon Hwang, Daehyun Kim, Oh Sun Kwon, Yun-Sun Kim, Byungjun Ahn, Nae-Gyu Kang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7819924323a64d95bc1d9cceb189ba48
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spelling	oai:doaj.org-article:7819924323a64d95bc1d9cceb189ba482021-12-02T12:33:15ZTopical application of Zanthoxylum piperitum extract improves lateral canthal rhytides by inhibiting muscle contractions10.1038/s41598-020-78610-w2045-2322https://doaj.org/article/7819924323a64d95bc1d9cceb189ba482020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78610-whttps://doaj.org/toc/2045-2322Abstract Facial wrinkles are the predominant phenotypes of skin aging. To date, one of the most effective ways to improve wrinkles is botulinum toxin type A (BoNT/A) injection, which inhibits muscle contractions by reducing acetylcholine release from neurons. However, since BoNT/A is a hazardous neurotoxin, the injection can only be performed by medical doctors and the procedure is only possible through invasive injection, causing inconveniences such as pain. To overcome these inconveniences, we tried to find a way to reduce wrinkles non-invasively via mechanisms similar to BoNT/A. We first designed in vitro assays to test BoNT/A-like muscle contraction inhibition in two different model systems. By using the assays, we identified Zanthoxylum piperitum (Z. piperitum) fruit extract as a BoNT-like reagent (27.7% decrease of muscle contraction rates by 1000 ppm of Z. piperitum extract treatment). Next, we determined mechanisms of how Z. piperitum extract decreases muscle contraction rates and found that the extract treatment inhibits electrical signal transduction in neurons. We also showed that among known components of Z. piperitum extract, quercitrin is responsible for muscle contraction inhibition. We further identified that Z. piperitum extract has synergistic effects with acetyl hexapeptide-8 and BoNT/A light chain, which are well-known BoNT-like peptides. Finally, we showed that topical treatment of the Z. piperitum extract indeed decreases facial wrinkles and treatment of Z. piperitum extract with acetyl hexapeptide-8 has a tendency to improve wrinkles synergistically (14.5% improvement on average). The synergistic effect of the combination is expected to improve wrinkles effectively by implementing the BoNT/A mechanisms in a non-invasive way.Wooseon HwangDaehyun KimOh Sun KwonYun-Sun KimByungjun AhnNae-Gyu KangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-8 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wooseon Hwang Daehyun Kim Oh Sun Kwon Yun-Sun Kim Byungjun Ahn Nae-Gyu Kang Topical application of Zanthoxylum piperitum extract improves lateral canthal rhytides by inhibiting muscle contractions
description	Abstract Facial wrinkles are the predominant phenotypes of skin aging. To date, one of the most effective ways to improve wrinkles is botulinum toxin type A (BoNT/A) injection, which inhibits muscle contractions by reducing acetylcholine release from neurons. However, since BoNT/A is a hazardous neurotoxin, the injection can only be performed by medical doctors and the procedure is only possible through invasive injection, causing inconveniences such as pain. To overcome these inconveniences, we tried to find a way to reduce wrinkles non-invasively via mechanisms similar to BoNT/A. We first designed in vitro assays to test BoNT/A-like muscle contraction inhibition in two different model systems. By using the assays, we identified Zanthoxylum piperitum (Z. piperitum) fruit extract as a BoNT-like reagent (27.7% decrease of muscle contraction rates by 1000 ppm of Z. piperitum extract treatment). Next, we determined mechanisms of how Z. piperitum extract decreases muscle contraction rates and found that the extract treatment inhibits electrical signal transduction in neurons. We also showed that among known components of Z. piperitum extract, quercitrin is responsible for muscle contraction inhibition. We further identified that Z. piperitum extract has synergistic effects with acetyl hexapeptide-8 and BoNT/A light chain, which are well-known BoNT-like peptides. Finally, we showed that topical treatment of the Z. piperitum extract indeed decreases facial wrinkles and treatment of Z. piperitum extract with acetyl hexapeptide-8 has a tendency to improve wrinkles synergistically (14.5% improvement on average). The synergistic effect of the combination is expected to improve wrinkles effectively by implementing the BoNT/A mechanisms in a non-invasive way.
format	article
author	Wooseon Hwang Daehyun Kim Oh Sun Kwon Yun-Sun Kim Byungjun Ahn Nae-Gyu Kang
author_facet	Wooseon Hwang Daehyun Kim Oh Sun Kwon Yun-Sun Kim Byungjun Ahn Nae-Gyu Kang
author_sort	Wooseon Hwang
title	Topical application of Zanthoxylum piperitum extract improves lateral canthal rhytides by inhibiting muscle contractions
title_short	Topical application of Zanthoxylum piperitum extract improves lateral canthal rhytides by inhibiting muscle contractions
title_full	Topical application of Zanthoxylum piperitum extract improves lateral canthal rhytides by inhibiting muscle contractions
title_fullStr	Topical application of Zanthoxylum piperitum extract improves lateral canthal rhytides by inhibiting muscle contractions
title_full_unstemmed	Topical application of Zanthoxylum piperitum extract improves lateral canthal rhytides by inhibiting muscle contractions
title_sort	topical application of zanthoxylum piperitum extract improves lateral canthal rhytides by inhibiting muscle contractions
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/7819924323a64d95bc1d9cceb189ba48
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Topical application of Zanthoxylum piperitum extract improves lateral canthal rhytides by inhibiting muscle contractions

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