Histone deacetylase 3 depletion in osteo/chondroprogenitor cells decreases bone density and increases marrow fat.

Histone deacetylase (Hdac)3 is a nuclear enzyme that contributes to epigenetic programming and is required for embryonic development. To determine the role of Hdac3 in bone formation, we crossed mice harboring loxP sites around exon 7 of Hdac3 with mice expressing Cre recombinase under the control o...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	David F Razidlo, Tiffany J Whitney, Michelle E Casper, Meghan E McGee-Lawrence, Bridget A Stensgard, Xiaodong Li, Frank J Secreto, Sarah K Knutson, Scott W Hiebert, Jennifer J Westendorf
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/782d7d2bcd6441dc8442d80bda9aaaf9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:782d7d2bcd6441dc8442d80bda9aaaf9
record_format	dspace
spelling	oai:doaj.org-article:782d7d2bcd6441dc8442d80bda9aaaf92021-12-02T20:20:13ZHistone deacetylase 3 depletion in osteo/chondroprogenitor cells decreases bone density and increases marrow fat.1932-620310.1371/journal.pone.0011492https://doaj.org/article/782d7d2bcd6441dc8442d80bda9aaaf92010-07-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20628553/?tool=EBIhttps://doaj.org/toc/1932-6203Histone deacetylase (Hdac)3 is a nuclear enzyme that contributes to epigenetic programming and is required for embryonic development. To determine the role of Hdac3 in bone formation, we crossed mice harboring loxP sites around exon 7 of Hdac3 with mice expressing Cre recombinase under the control of the osterix promoter. The resulting Hdac3 conditional knockout (CKO) mice were runted and had severe deficits in intramembranous and endochondral bone formation. Calvarial bones were significantly thinner and trabecular bone volume in the distal femur was decreased 75% in the Hdac3 CKO mice due to a substantial reduction in trabecular number. Hdac3-CKO mice had fewer osteoblasts and more bone marrow adipocytes as a proportion of tissue area than their wildtype or heterozygous littermates. Bone formation rates were depressed in both the cortical and trabecular regions of Hdac3 CKO femurs. Microarray analyses revealed that numerous developmental signaling pathways were affected by Hdac3-deficiency. Thus, Hdac3 depletion in osterix-expressing progenitor cells interferes with bone formation and promotes bone marrow adipocyte differentiation. These results demonstrate that Hdac3 inhibition is detrimental to skeletal health.David F RazidloTiffany J WhitneyMichelle E CasperMeghan E McGee-LawrenceBridget A StensgardXiaodong LiFrank J SecretoSarah K KnutsonScott W HiebertJennifer J WestendorfPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 7, p e11492 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q David F Razidlo Tiffany J Whitney Michelle E Casper Meghan E McGee-Lawrence Bridget A Stensgard Xiaodong Li Frank J Secreto Sarah K Knutson Scott W Hiebert Jennifer J Westendorf Histone deacetylase 3 depletion in osteo/chondroprogenitor cells decreases bone density and increases marrow fat.
description	Histone deacetylase (Hdac)3 is a nuclear enzyme that contributes to epigenetic programming and is required for embryonic development. To determine the role of Hdac3 in bone formation, we crossed mice harboring loxP sites around exon 7 of Hdac3 with mice expressing Cre recombinase under the control of the osterix promoter. The resulting Hdac3 conditional knockout (CKO) mice were runted and had severe deficits in intramembranous and endochondral bone formation. Calvarial bones were significantly thinner and trabecular bone volume in the distal femur was decreased 75% in the Hdac3 CKO mice due to a substantial reduction in trabecular number. Hdac3-CKO mice had fewer osteoblasts and more bone marrow adipocytes as a proportion of tissue area than their wildtype or heterozygous littermates. Bone formation rates were depressed in both the cortical and trabecular regions of Hdac3 CKO femurs. Microarray analyses revealed that numerous developmental signaling pathways were affected by Hdac3-deficiency. Thus, Hdac3 depletion in osterix-expressing progenitor cells interferes with bone formation and promotes bone marrow adipocyte differentiation. These results demonstrate that Hdac3 inhibition is detrimental to skeletal health.
format	article
author	David F Razidlo Tiffany J Whitney Michelle E Casper Meghan E McGee-Lawrence Bridget A Stensgard Xiaodong Li Frank J Secreto Sarah K Knutson Scott W Hiebert Jennifer J Westendorf
author_facet	David F Razidlo Tiffany J Whitney Michelle E Casper Meghan E McGee-Lawrence Bridget A Stensgard Xiaodong Li Frank J Secreto Sarah K Knutson Scott W Hiebert Jennifer J Westendorf
author_sort	David F Razidlo
title	Histone deacetylase 3 depletion in osteo/chondroprogenitor cells decreases bone density and increases marrow fat.
title_short	Histone deacetylase 3 depletion in osteo/chondroprogenitor cells decreases bone density and increases marrow fat.
title_full	Histone deacetylase 3 depletion in osteo/chondroprogenitor cells decreases bone density and increases marrow fat.
title_fullStr	Histone deacetylase 3 depletion in osteo/chondroprogenitor cells decreases bone density and increases marrow fat.
title_full_unstemmed	Histone deacetylase 3 depletion in osteo/chondroprogenitor cells decreases bone density and increases marrow fat.
title_sort	histone deacetylase 3 depletion in osteo/chondroprogenitor cells decreases bone density and increases marrow fat.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/782d7d2bcd6441dc8442d80bda9aaaf9
work_keys_str_mv	AT davidfrazidlo histonedeacetylase3depletioninosteochondroprogenitorcellsdecreasesbonedensityandincreasesmarrowfat AT tiffanyjwhitney histonedeacetylase3depletioninosteochondroprogenitorcellsdecreasesbonedensityandincreasesmarrowfat AT michelleecasper histonedeacetylase3depletioninosteochondroprogenitorcellsdecreasesbonedensityandincreasesmarrowfat AT meghanemcgeelawrence histonedeacetylase3depletioninosteochondroprogenitorcellsdecreasesbonedensityandincreasesmarrowfat AT bridgetastensgard histonedeacetylase3depletioninosteochondroprogenitorcellsdecreasesbonedensityandincreasesmarrowfat AT xiaodongli histonedeacetylase3depletioninosteochondroprogenitorcellsdecreasesbonedensityandincreasesmarrowfat AT frankjsecreto histonedeacetylase3depletioninosteochondroprogenitorcellsdecreasesbonedensityandincreasesmarrowfat AT sarahkknutson histonedeacetylase3depletioninosteochondroprogenitorcellsdecreasesbonedensityandincreasesmarrowfat AT scottwhiebert histonedeacetylase3depletioninosteochondroprogenitorcellsdecreasesbonedensityandincreasesmarrowfat AT jenniferjwestendorf histonedeacetylase3depletioninosteochondroprogenitorcellsdecreasesbonedensityandincreasesmarrowfat
_version_	1718374171712421888

Histone deacetylase 3 depletion in osteo/chondroprogenitor cells decreases bone density and increases marrow fat.

Ejemplares similares