Immunohystochemical study of the largest islets of human pancreas in aging and diabetes mellitus: perspectives for the transplantation

Aim. To study the distribution and cellular architecture of the largest human pancreatic islets (with a diameter of more than 200 micron) in aging and diabetes mellitus types 1 and 2. Materials and methods. Antibodies to insulin, glucagon, somastatin and nturon-specific enolase were applied. The...

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Autores principales:	Alexandra Evgen'evna Proshchina, Yulia Sergeevna Krivova, Valeriy Mikhaylovich Barabanov, Sergey Vyacheslavovich Savelyev
Formato:	article
Lenguaje:	EN RU
Publicado:	Endocrinology Research Centre 2013
Materias:	islets diabetes mellitus aging insulin glucagon somatostatin neuron-specific enolase nse transplantation Nutritional diseases. Deficiency diseases RC620-627
Acceso en línea:	https://doaj.org/article/783a57cd494e4763838ae2a1242a6119
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spelling	oai:doaj.org-article:783a57cd494e4763838ae2a1242a61192021-11-14T09:00:18ZImmunohystochemical study of the largest islets of human pancreas in aging and diabetes mellitus: perspectives for the transplantation2072-03512072-037810.14341/DM2013438-43https://doaj.org/article/783a57cd494e4763838ae2a1242a61192013-12-01T00:00:00Zhttps://www.dia-endojournals.ru/jour/article/view/6270https://doaj.org/toc/2072-0351https://doaj.org/toc/2072-0378Aim. To study the distribution and cellular architecture of the largest human pancreatic islets (with a diameter of more than 200 micron) in aging and diabetes mellitus types 1 and 2. Materials and methods. Antibodies to insulin, glucagon, somastatin and nturon-specific enolase were applied. The autopsy samples of the pancreatic tissue of patients with diabetes mellitus type 1 (DMT1) and type 2 (DMT2) and 2 age groups (up to 50 years old (control) and after 50 (aging control)), not suffering from diseases of the pancreas and carbohydrate metabolism malfunction were investigated. Results. The number of islets with diameter more than 200 mkm compared to control group increased both in aging and diabetes groups. Their number reaches in some cases 15% (and higher in DM) of the total number of islets. These islets compared to the other are rich-vascularized. It was shown that glucagon and somatostatin-containing cells are found both on the periphery of the large islets, and inside them only in the immediate proximity of the capillaries. Insulin-containing cells form clusters, surrounded by the capillaries and the ?- and ?-cells, while the inner part of such clusters has no direct contact with the capillaries. In the large islets the number of glucagon-containing cells is often increased, and insulin-containing cells show signs of degradation. Conclusion. The largest of the pancreatic islets may be useless for the transplantation, because of the high content of glucagon-containing cells, the rich vascularization and, in some cases, the limited functionality of ?-cells.Alexandra Evgen'evna ProshchinaYulia Sergeevna KrivovaValeriy Mikhaylovich BarabanovSergey Vyacheslavovich SavelyevEndocrinology Research Centrearticleisletsdiabetes mellitusaginginsulinglucagonsomatostatinneuron-specific enolasensetransplantationNutritional diseases. Deficiency diseasesRC620-627ENRUСахарный диабет, Vol 16, Iss 4, Pp 38-43 (2013)
institution	DOAJ
collection	DOAJ
language	EN RU
topic	islets diabetes mellitus aging insulin glucagon somatostatin neuron-specific enolase nse transplantation Nutritional diseases. Deficiency diseases RC620-627
spellingShingle	islets diabetes mellitus aging insulin glucagon somatostatin neuron-specific enolase nse transplantation Nutritional diseases. Deficiency diseases RC620-627 Alexandra Evgen'evna Proshchina Yulia Sergeevna Krivova Valeriy Mikhaylovich Barabanov Sergey Vyacheslavovich Savelyev Immunohystochemical study of the largest islets of human pancreas in aging and diabetes mellitus: perspectives for the transplantation
description	Aim. To study the distribution and cellular architecture of the largest human pancreatic islets (with a diameter of more than 200 micron) in aging and diabetes mellitus types 1 and 2. Materials and methods. Antibodies to insulin, glucagon, somastatin and nturon-specific enolase were applied. The autopsy samples of the pancreatic tissue of patients with diabetes mellitus type 1 (DMT1) and type 2 (DMT2) and 2 age groups (up to 50 years old (control) and after 50 (aging control)), not suffering from diseases of the pancreas and carbohydrate metabolism malfunction were investigated. Results. The number of islets with diameter more than 200 mkm compared to control group increased both in aging and diabetes groups. Their number reaches in some cases 15% (and higher in DM) of the total number of islets. These islets compared to the other are rich-vascularized. It was shown that glucagon and somatostatin-containing cells are found both on the periphery of the large islets, and inside them only in the immediate proximity of the capillaries. Insulin-containing cells form clusters, surrounded by the capillaries and the ?- and ?-cells, while the inner part of such clusters has no direct contact with the capillaries. In the large islets the number of glucagon-containing cells is often increased, and insulin-containing cells show signs of degradation. Conclusion. The largest of the pancreatic islets may be useless for the transplantation, because of the high content of glucagon-containing cells, the rich vascularization and, in some cases, the limited functionality of ?-cells.
format	article
author	Alexandra Evgen'evna Proshchina Yulia Sergeevna Krivova Valeriy Mikhaylovich Barabanov Sergey Vyacheslavovich Savelyev
author_facet	Alexandra Evgen'evna Proshchina Yulia Sergeevna Krivova Valeriy Mikhaylovich Barabanov Sergey Vyacheslavovich Savelyev
author_sort	Alexandra Evgen'evna Proshchina
title	Immunohystochemical study of the largest islets of human pancreas in aging and diabetes mellitus: perspectives for the transplantation
title_short	Immunohystochemical study of the largest islets of human pancreas in aging and diabetes mellitus: perspectives for the transplantation
title_full	Immunohystochemical study of the largest islets of human pancreas in aging and diabetes mellitus: perspectives for the transplantation
title_fullStr	Immunohystochemical study of the largest islets of human pancreas in aging and diabetes mellitus: perspectives for the transplantation
title_full_unstemmed	Immunohystochemical study of the largest islets of human pancreas in aging and diabetes mellitus: perspectives for the transplantation
title_sort	immunohystochemical study of the largest islets of human pancreas in aging and diabetes mellitus: perspectives for the transplantation
publisher	Endocrinology Research Centre
publishDate	2013
url	https://doaj.org/article/783a57cd494e4763838ae2a1242a6119
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Immunohystochemical study of the largest islets of human pancreas in aging and diabetes mellitus: perspectives for the transplantation

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