Transcriptome and phytochemical analyses provide insights into the organic sulfur pathway in Allium hirtifolium

Abstract Allium is one of the well-known genera of the Amaryllidaceae family, which contains over 780 species. Onions, garlic, leeks, and shallots are the most important species of this genus. Allium hirtifolium (shallot) is a rich source of proteins, carbohydrates, lipids, amino acids, and bioactiv...

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Autores principales:	Aboozar Soorni, Amir Mohammad Akrami, Reza Abolghasemi, Maryam Vahedi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/783ab65b033a4ddfa37b74b6184b491f
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spelling	oai:doaj.org-article:783ab65b033a4ddfa37b74b6184b491f2021-12-02T14:01:23ZTranscriptome and phytochemical analyses provide insights into the organic sulfur pathway in Allium hirtifolium10.1038/s41598-020-80837-62045-2322https://doaj.org/article/783ab65b033a4ddfa37b74b6184b491f2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80837-6https://doaj.org/toc/2045-2322Abstract Allium is one of the well-known genera of the Amaryllidaceae family, which contains over 780 species. Onions, garlic, leeks, and shallots are the most important species of this genus. Allium hirtifolium (shallot) is a rich source of proteins, carbohydrates, lipids, amino acids, and bioactive compounds such as organic sulfur compounds with an expansive range of biological activities and medicinal attributes. To identify the putative compounds and genes involved in the organic sulfur pathway, we applied GC–MS and RNA-seq techniques for the bulb, stem, and flower tissues of A. hirtifolium. The essential oil analysis revealed the maximum amount of sulfur compounds in stem against flower and bulb tissues. Transcriptome profiling showed 6155, 6494, and 4259 DEGs for bulb vs. flower, bulb vs. stem, and flower vs. stem, respectively. Overall, more genes were identified as being up-regulated rather than down-regulated in flower tissue compared to the stem and bulb tissues. Our findings in accordance with other results from different papers, suggest that carbohydrates are vital to bulb formation and development because a high number of identified DEGs (586 genes) were mapped to carbohydrate metabolism. This study has detected the genes in the organic sulfur pathway and indicated that the alliinase gene shows a high variability among different tissues. In general, this study formed a useful genomic resource data to explore tissue-specific sulfur pathway in A. hirtifolium, which is helpful for functional breeding.Aboozar SoorniAmir Mohammad AkramiReza AbolghasemiMaryam VahediNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Aboozar Soorni Amir Mohammad Akrami Reza Abolghasemi Maryam Vahedi Transcriptome and phytochemical analyses provide insights into the organic sulfur pathway in Allium hirtifolium
description	Abstract Allium is one of the well-known genera of the Amaryllidaceae family, which contains over 780 species. Onions, garlic, leeks, and shallots are the most important species of this genus. Allium hirtifolium (shallot) is a rich source of proteins, carbohydrates, lipids, amino acids, and bioactive compounds such as organic sulfur compounds with an expansive range of biological activities and medicinal attributes. To identify the putative compounds and genes involved in the organic sulfur pathway, we applied GC–MS and RNA-seq techniques for the bulb, stem, and flower tissues of A. hirtifolium. The essential oil analysis revealed the maximum amount of sulfur compounds in stem against flower and bulb tissues. Transcriptome profiling showed 6155, 6494, and 4259 DEGs for bulb vs. flower, bulb vs. stem, and flower vs. stem, respectively. Overall, more genes were identified as being up-regulated rather than down-regulated in flower tissue compared to the stem and bulb tissues. Our findings in accordance with other results from different papers, suggest that carbohydrates are vital to bulb formation and development because a high number of identified DEGs (586 genes) were mapped to carbohydrate metabolism. This study has detected the genes in the organic sulfur pathway and indicated that the alliinase gene shows a high variability among different tissues. In general, this study formed a useful genomic resource data to explore tissue-specific sulfur pathway in A. hirtifolium, which is helpful for functional breeding.
format	article
author	Aboozar Soorni Amir Mohammad Akrami Reza Abolghasemi Maryam Vahedi
author_facet	Aboozar Soorni Amir Mohammad Akrami Reza Abolghasemi Maryam Vahedi
author_sort	Aboozar Soorni
title	Transcriptome and phytochemical analyses provide insights into the organic sulfur pathway in Allium hirtifolium
title_short	Transcriptome and phytochemical analyses provide insights into the organic sulfur pathway in Allium hirtifolium
title_full	Transcriptome and phytochemical analyses provide insights into the organic sulfur pathway in Allium hirtifolium
title_fullStr	Transcriptome and phytochemical analyses provide insights into the organic sulfur pathway in Allium hirtifolium
title_full_unstemmed	Transcriptome and phytochemical analyses provide insights into the organic sulfur pathway in Allium hirtifolium
title_sort	transcriptome and phytochemical analyses provide insights into the organic sulfur pathway in allium hirtifolium
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/783ab65b033a4ddfa37b74b6184b491f
work_keys_str_mv	AT aboozarsoorni transcriptomeandphytochemicalanalysesprovideinsightsintotheorganicsulfurpathwayinalliumhirtifolium AT amirmohammadakrami transcriptomeandphytochemicalanalysesprovideinsightsintotheorganicsulfurpathwayinalliumhirtifolium AT rezaabolghasemi transcriptomeandphytochemicalanalysesprovideinsightsintotheorganicsulfurpathwayinalliumhirtifolium AT maryamvahedi transcriptomeandphytochemicalanalysesprovideinsightsintotheorganicsulfurpathwayinalliumhirtifolium
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Transcriptome and phytochemical analyses provide insights into the organic sulfur pathway in Allium hirtifolium

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