Evaluation of endothelial/Descemet membrane complex of eye bank donor corneas using enhanced depth imaging optical coherence tomography

Zeba A Syed,1 Gustavo Rosa Gameiro,1 Marco Ruggeri,1 Amr Elsawy,1,2 Ibrahim Sayed-Ahmed,1 Vatookarn Roongpoovapatr,1 Mohamed Abdel-Mottaleb,2 Mohamed Abou Shousha11Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, USA; 2Department of...

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Autores principales:	Syed ZA, Gameiro GR, Ruggeri M, Elsawy A, Sayed-Ahmed I, Roongpoovapatr V, Abdel-Mottaleb M, Abou Shousha M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Corneal transplant Fuchs’ endothelial corneal dystrophy corneal graft screening microscope-integrated OCT Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/783ca99219e54b52af1e0a7f7f7daecf
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spelling	oai:doaj.org-article:783ca99219e54b52af1e0a7f7f7daecf2021-12-02T03:15:53ZEvaluation of endothelial/Descemet membrane complex of eye bank donor corneas using enhanced depth imaging optical coherence tomography1177-5483https://doaj.org/article/783ca99219e54b52af1e0a7f7f7daecf2019-05-01T00:00:00Zhttps://www.dovepress.com/evaluation-of-endothelialdescemet-membrane-complex-of-eye-bank-donor-c-peer-reviewed-article-OPTHhttps://doaj.org/toc/1177-5483Zeba A Syed,1 Gustavo Rosa Gameiro,1 Marco Ruggeri,1 Amr Elsawy,1,2 Ibrahim Sayed-Ahmed,1 Vatookarn Roongpoovapatr,1 Mohamed Abdel-Mottaleb,2 Mohamed Abou Shousha11Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, USA; 2Department of Electrical and Computer Engineering, University of Miami College of Engineering, Coral Gables, FL, USAObjective: We present a novel method for screening eye bank donor corneas using high definition optical coherence tomography (HD-OCT). This technology allows for the quantification of endothelial/Descemet membrane (En/DM) complex thickness ex vivo.Design: Prospective interventional study.Participants: Fifty-two corneal grafts from 27 donors were included in this study. Twenty additional control eyes and 11 eyes with Fuchs’ endothelial corneal dystrophy were also evaluated for comparison.Methods: A custom built, high speed HD-OCT device (Envisu R2210, Bioptigen, Buffalo Grove, IL, USA) was used to obtain images, and custom-made graph-based segmentation software was used to automatically deconstruct corneal images into micro-layers. HD-OCT imaging was used to scan through the sealed sterile case of donor corneas stored in McCarey-Kaufman medium to image their En/DM complex through the center of the cornea.Results: This technology allowed for quantification of En/DM complex thickness in all donor corneas through the sealed sterile container used to transport graft tissue. Mean En/DM complex thickness of donor corneas was 17±4 μm. The difference between donor cornea En/DM thickness and that of control subjects (16±2 μm) was not statistically significant (p=0.3), suggesting that the transport container and media do not affect measurements. There was a significant difference between En/DM thickness of Fuchs’ endothelial corneal dystrophy eyes (25±5 μm) and both donor corneas (p<0.0001) and control subjects (p<0.0001).Conclusions: We have described a new technique to measure En/DM complex thickness in eye bank donor corneas stored in a sealed sterile case. This may represent a novel adjunctive approach to screen corneal grafts for early endothelial disease.Keywords: corneal transplant, Fuchs’ endothelial corneal dystrophy, corneal graft screening, microscope-integrated OCTSyed ZAGameiro GRRuggeri MElsawy ASayed-Ahmed IRoongpoovapatr VAbdel-Mottaleb MAbou Shousha MDove Medical PressarticleCorneal transplantFuchs’ endothelial corneal dystrophycorneal graft screeningmicroscope-integrated OCTOphthalmologyRE1-994ENClinical Ophthalmology, Vol Volume 13, Pp 789-794 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Corneal transplant Fuchs’ endothelial corneal dystrophy corneal graft screening microscope-integrated OCT Ophthalmology RE1-994
spellingShingle	Corneal transplant Fuchs’ endothelial corneal dystrophy corneal graft screening microscope-integrated OCT Ophthalmology RE1-994 Syed ZA Gameiro GR Ruggeri M Elsawy A Sayed-Ahmed I Roongpoovapatr V Abdel-Mottaleb M Abou Shousha M Evaluation of endothelial/Descemet membrane complex of eye bank donor corneas using enhanced depth imaging optical coherence tomography
description	Zeba A Syed,1 Gustavo Rosa Gameiro,1 Marco Ruggeri,1 Amr Elsawy,1,2 Ibrahim Sayed-Ahmed,1 Vatookarn Roongpoovapatr,1 Mohamed Abdel-Mottaleb,2 Mohamed Abou Shousha11Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, USA; 2Department of Electrical and Computer Engineering, University of Miami College of Engineering, Coral Gables, FL, USAObjective: We present a novel method for screening eye bank donor corneas using high definition optical coherence tomography (HD-OCT). This technology allows for the quantification of endothelial/Descemet membrane (En/DM) complex thickness ex vivo.Design: Prospective interventional study.Participants: Fifty-two corneal grafts from 27 donors were included in this study. Twenty additional control eyes and 11 eyes with Fuchs’ endothelial corneal dystrophy were also evaluated for comparison.Methods: A custom built, high speed HD-OCT device (Envisu R2210, Bioptigen, Buffalo Grove, IL, USA) was used to obtain images, and custom-made graph-based segmentation software was used to automatically deconstruct corneal images into micro-layers. HD-OCT imaging was used to scan through the sealed sterile case of donor corneas stored in McCarey-Kaufman medium to image their En/DM complex through the center of the cornea.Results: This technology allowed for quantification of En/DM complex thickness in all donor corneas through the sealed sterile container used to transport graft tissue. Mean En/DM complex thickness of donor corneas was 17±4 μm. The difference between donor cornea En/DM thickness and that of control subjects (16±2 μm) was not statistically significant (p=0.3), suggesting that the transport container and media do not affect measurements. There was a significant difference between En/DM thickness of Fuchs’ endothelial corneal dystrophy eyes (25±5 μm) and both donor corneas (p<0.0001) and control subjects (p<0.0001).Conclusions: We have described a new technique to measure En/DM complex thickness in eye bank donor corneas stored in a sealed sterile case. This may represent a novel adjunctive approach to screen corneal grafts for early endothelial disease.Keywords: corneal transplant, Fuchs’ endothelial corneal dystrophy, corneal graft screening, microscope-integrated OCT
format	article
author	Syed ZA Gameiro GR Ruggeri M Elsawy A Sayed-Ahmed I Roongpoovapatr V Abdel-Mottaleb M Abou Shousha M
author_facet	Syed ZA Gameiro GR Ruggeri M Elsawy A Sayed-Ahmed I Roongpoovapatr V Abdel-Mottaleb M Abou Shousha M
author_sort	Syed ZA
title	Evaluation of endothelial/Descemet membrane complex of eye bank donor corneas using enhanced depth imaging optical coherence tomography
title_short	Evaluation of endothelial/Descemet membrane complex of eye bank donor corneas using enhanced depth imaging optical coherence tomography
title_full	Evaluation of endothelial/Descemet membrane complex of eye bank donor corneas using enhanced depth imaging optical coherence tomography
title_fullStr	Evaluation of endothelial/Descemet membrane complex of eye bank donor corneas using enhanced depth imaging optical coherence tomography
title_full_unstemmed	Evaluation of endothelial/Descemet membrane complex of eye bank donor corneas using enhanced depth imaging optical coherence tomography
title_sort	evaluation of endothelial/descemet membrane complex of eye bank donor corneas using enhanced depth imaging optical coherence tomography
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/783ca99219e54b52af1e0a7f7f7daecf
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Evaluation of endothelial/Descemet membrane complex of eye bank donor corneas using enhanced depth imaging optical coherence tomography

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