The Effect of Mycotoxins and Silymarin on Liver Lipidome of Mice with Non-Alcoholic Fatty Liver Disease

Milk thistle-based dietary supplements have become increasingly popular. The extract from milk thistle (<i>Silybum marianum</i>) is often used for the treatment of liver diseases because of the presence of its active component, silymarin. However, the co-occurrence of toxic mycotoxins in...

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Autores principales:	Kamila Bechynska, Vit Kosek, Marie Fenclova, Lucie Muchova, Vaclav Smid, Jakub Suk, Karel Chalupsky, Eva Sticova, Kamila Hurkova, Jana Hajslova, Libor Vitek, Milena Stranska
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	mycotoxins silymarin lipidome metabolome mice liver mass spectrometry Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/783e6e9e5ffe452894cf65d603c43fb2
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spelling	oai:doaj.org-article:783e6e9e5ffe452894cf65d603c43fb22021-11-25T16:54:25ZThe Effect of Mycotoxins and Silymarin on Liver Lipidome of Mice with Non-Alcoholic Fatty Liver Disease10.3390/biom111117232218-273Xhttps://doaj.org/article/783e6e9e5ffe452894cf65d603c43fb22021-11-01T00:00:00Zhttps://www.mdpi.com/2218-273X/11/11/1723https://doaj.org/toc/2218-273XMilk thistle-based dietary supplements have become increasingly popular. The extract from milk thistle (<i>Silybum marianum</i>) is often used for the treatment of liver diseases because of the presence of its active component, silymarin. However, the co-occurrence of toxic mycotoxins in these preparations is quite frequent as well. The objective of this study was to investigate the changes in composition of liver lipidome and other clinical characteristics of experimental mice fed by a high-fat methionine-choline deficient diet inducing non-alcoholic fatty liver disease. The mice were exposed to (i) silymarin, (ii) mycotoxins (trichothecenes, enniatins, beauvericin, and altertoxins) and (iii) both silymarin and mycotoxins, and results were compared to the controls. The liver tissue extracts were analyzed by ultra-high performance liquid chromatography coupled with high-resolution tandem mass spectrometry. Using tools of univariate and multivariate statistical analysis, we were able to identify 48 lipid species from the classes of diacylglycerols, triacylglycerols, free fatty acids, fatty acid esters of hydroxy fatty acids and phospholipids clearly reflecting the dysregulation of lipid metabolism upon exposure to mycotoxin and/or silymarin.Kamila BechynskaVit KosekMarie FenclovaLucie MuchovaVaclav SmidJakub SukKarel ChalupskyEva SticovaKamila HurkovaJana HajslovaLibor VitekMilena StranskaMDPI AGarticlemycotoxinssilymarinlipidomemetabolomemice livermass spectrometryMicrobiologyQR1-502ENBiomolecules, Vol 11, Iss 1723, p 1723 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	mycotoxins silymarin lipidome metabolome mice liver mass spectrometry Microbiology QR1-502
spellingShingle	mycotoxins silymarin lipidome metabolome mice liver mass spectrometry Microbiology QR1-502 Kamila Bechynska Vit Kosek Marie Fenclova Lucie Muchova Vaclav Smid Jakub Suk Karel Chalupsky Eva Sticova Kamila Hurkova Jana Hajslova Libor Vitek Milena Stranska The Effect of Mycotoxins and Silymarin on Liver Lipidome of Mice with Non-Alcoholic Fatty Liver Disease
description	Milk thistle-based dietary supplements have become increasingly popular. The extract from milk thistle (<i>Silybum marianum</i>) is often used for the treatment of liver diseases because of the presence of its active component, silymarin. However, the co-occurrence of toxic mycotoxins in these preparations is quite frequent as well. The objective of this study was to investigate the changes in composition of liver lipidome and other clinical characteristics of experimental mice fed by a high-fat methionine-choline deficient diet inducing non-alcoholic fatty liver disease. The mice were exposed to (i) silymarin, (ii) mycotoxins (trichothecenes, enniatins, beauvericin, and altertoxins) and (iii) both silymarin and mycotoxins, and results were compared to the controls. The liver tissue extracts were analyzed by ultra-high performance liquid chromatography coupled with high-resolution tandem mass spectrometry. Using tools of univariate and multivariate statistical analysis, we were able to identify 48 lipid species from the classes of diacylglycerols, triacylglycerols, free fatty acids, fatty acid esters of hydroxy fatty acids and phospholipids clearly reflecting the dysregulation of lipid metabolism upon exposure to mycotoxin and/or silymarin.
format	article
author	Kamila Bechynska Vit Kosek Marie Fenclova Lucie Muchova Vaclav Smid Jakub Suk Karel Chalupsky Eva Sticova Kamila Hurkova Jana Hajslova Libor Vitek Milena Stranska
author_facet	Kamila Bechynska Vit Kosek Marie Fenclova Lucie Muchova Vaclav Smid Jakub Suk Karel Chalupsky Eva Sticova Kamila Hurkova Jana Hajslova Libor Vitek Milena Stranska
author_sort	Kamila Bechynska
title	The Effect of Mycotoxins and Silymarin on Liver Lipidome of Mice with Non-Alcoholic Fatty Liver Disease
title_short	The Effect of Mycotoxins and Silymarin on Liver Lipidome of Mice with Non-Alcoholic Fatty Liver Disease
title_full	The Effect of Mycotoxins and Silymarin on Liver Lipidome of Mice with Non-Alcoholic Fatty Liver Disease
title_fullStr	The Effect of Mycotoxins and Silymarin on Liver Lipidome of Mice with Non-Alcoholic Fatty Liver Disease
title_full_unstemmed	The Effect of Mycotoxins and Silymarin on Liver Lipidome of Mice with Non-Alcoholic Fatty Liver Disease
title_sort	effect of mycotoxins and silymarin on liver lipidome of mice with non-alcoholic fatty liver disease
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/783e6e9e5ffe452894cf65d603c43fb2
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The Effect of Mycotoxins and Silymarin on Liver Lipidome of Mice with Non-Alcoholic Fatty Liver Disease

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