Development of a bioaffinity SPR immunosensor based on functionalized graphene oxide for the detection of pregnancy-associated plasma protein A2 in human plasma

Nan-Fu Chiu,1 Ming-Jung Tai,1 Hwai-Ping Wu,1 Ting-Li Lin,1 Chen-Yu Chen2,31Laboratory of Nano-photonics and Biosensors, Institute of Electro-Optical Engineering, National Taiwan Normal University, Taipei 11677, Taiwan; 2Department of Obstetrics and Gynecology, Mackay Memorial Hospital, Taipei City 1...

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Autores principales:	Chiu NF, Tai MJ, Wu HP, Lin TL, Chen CY
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Carboxyl-graphene oxide (carboxyl-GO) surface plasmon resonance (SPR) pregnancy-associated plasma protein A2 (PAPPA2). Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/78437a5285a14d0baf4b1a3f38ea4a3f
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spelling	oai:doaj.org-article:78437a5285a14d0baf4b1a3f38ea4a3f2021-12-02T05:01:35ZDevelopment of a bioaffinity SPR immunosensor based on functionalized graphene oxide for the detection of pregnancy-associated plasma protein A2 in human plasma1178-2013https://doaj.org/article/78437a5285a14d0baf4b1a3f38ea4a3f2019-08-01T00:00:00Zhttps://www.dovepress.com/development-of-a-bioaffinity-spr-immunosensor-based-on-functionalized--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Nan-Fu Chiu,1 Ming-Jung Tai,1 Hwai-Ping Wu,1 Ting-Li Lin,1 Chen-Yu Chen2,31Laboratory of Nano-photonics and Biosensors, Institute of Electro-Optical Engineering, National Taiwan Normal University, Taipei 11677, Taiwan; 2Department of Obstetrics and Gynecology, Mackay Memorial Hospital, Taipei City 10449, Taiwan; 3Department of Medicine, Mackay Medical College, Taipei 25245, TaiwanCorrespondence: Nan-Fu ChiuLaboratory of Nano-photonics and Biosensors, Institute of Electro-Optical Engineering, National Taiwan Normal University, No. 88, Sec. 4, Ting-Chou Road, Taipei 11677, TaiwanTel +886 27 734 6722Fax +886 28 663 1954Email nfchiu@ntnu.edu.twBackground: Graphene-like material such as functionalized carboxyl-graphene oxide (carboxyl-GO) can be intelligently tuned to achieve particular properties for biological and chemical sensing applications.Methods: In this study, we propose a method to improve interference of non-specific proteins for use in human plasma assays. The highly specific interactions between molecules are an advantage of carboxyl-GO-based surface plasmon resonance (SPR) immunoassays, and this can be applied to spiked plasma samples with pregnancy-associated plasma protein A2 (PAPPA2).Results: The experiment results showed that carboxyl-GO could be used to modulate the plasmon resonance energy, work function and conductivity properties. In addition, carboxyl groups could be used to enhance the conduction of electrons between carboxyl-GO and Au electrodes due to the excellent conductivity and electron transfer rate. The carboxyl-GO-based SPR chip exhibited high sensitivity based on the electric field amplification effects of the composite dielectric material. Therefore, the surface electric field could be enhanced by electron transfer, thereby greatly improving the sensitivity of the sensing system. Enhanced electric field intensity was generated around the carboxyl-GO of 63.58 V/m, and the measured work function was 4.95 eV. The results showed that the carboxyl-GO-based SPR biosensor had high sensitivity, affinity and selective ability for PAPPA2 protein with a high association rate constant (ka) of 3.1 ×109, M-1, S-1, and a limit of detection of 0.01 pg/mL in spiked human plasma.Conclusion: The results showed a detection accuracy of protein in spiked plasma of >90% compared to PBS buffer, suggesting that the carboxyl-GO-based SPR biosensor could be used in assays of human plasma for early and late gynecological diseases. The future of this technology will be useful for the diagnosis and evaluation of the risk of early maternal preeclampsia and potentially in clinical applications for gestational diseases.Keywords: carboxyl, graphene oxide, carboxyl-GO, surface plasmon resonance, SPR, pregnancy, associated plasma protein A2, PAPPA2Chiu NFTai MJWu HPLin TLChen CYDove Medical PressarticleCarboxyl-graphene oxide (carboxyl-GO)surface plasmon resonance (SPR)pregnancy-associated plasma protein A2 (PAPPA2).Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 6735-6748 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Carboxyl-graphene oxide (carboxyl-GO) surface plasmon resonance (SPR) pregnancy-associated plasma protein A2 (PAPPA2). Medicine (General) R5-920
spellingShingle	Carboxyl-graphene oxide (carboxyl-GO) surface plasmon resonance (SPR) pregnancy-associated plasma protein A2 (PAPPA2). Medicine (General) R5-920 Chiu NF Tai MJ Wu HP Lin TL Chen CY Development of a bioaffinity SPR immunosensor based on functionalized graphene oxide for the detection of pregnancy-associated plasma protein A2 in human plasma
description	Nan-Fu Chiu,1 Ming-Jung Tai,1 Hwai-Ping Wu,1 Ting-Li Lin,1 Chen-Yu Chen2,31Laboratory of Nano-photonics and Biosensors, Institute of Electro-Optical Engineering, National Taiwan Normal University, Taipei 11677, Taiwan; 2Department of Obstetrics and Gynecology, Mackay Memorial Hospital, Taipei City 10449, Taiwan; 3Department of Medicine, Mackay Medical College, Taipei 25245, TaiwanCorrespondence: Nan-Fu ChiuLaboratory of Nano-photonics and Biosensors, Institute of Electro-Optical Engineering, National Taiwan Normal University, No. 88, Sec. 4, Ting-Chou Road, Taipei 11677, TaiwanTel +886 27 734 6722Fax +886 28 663 1954Email nfchiu@ntnu.edu.twBackground: Graphene-like material such as functionalized carboxyl-graphene oxide (carboxyl-GO) can be intelligently tuned to achieve particular properties for biological and chemical sensing applications.Methods: In this study, we propose a method to improve interference of non-specific proteins for use in human plasma assays. The highly specific interactions between molecules are an advantage of carboxyl-GO-based surface plasmon resonance (SPR) immunoassays, and this can be applied to spiked plasma samples with pregnancy-associated plasma protein A2 (PAPPA2).Results: The experiment results showed that carboxyl-GO could be used to modulate the plasmon resonance energy, work function and conductivity properties. In addition, carboxyl groups could be used to enhance the conduction of electrons between carboxyl-GO and Au electrodes due to the excellent conductivity and electron transfer rate. The carboxyl-GO-based SPR chip exhibited high sensitivity based on the electric field amplification effects of the composite dielectric material. Therefore, the surface electric field could be enhanced by electron transfer, thereby greatly improving the sensitivity of the sensing system. Enhanced electric field intensity was generated around the carboxyl-GO of 63.58 V/m, and the measured work function was 4.95 eV. The results showed that the carboxyl-GO-based SPR biosensor had high sensitivity, affinity and selective ability for PAPPA2 protein with a high association rate constant (ka) of 3.1 ×109, M-1, S-1, and a limit of detection of 0.01 pg/mL in spiked human plasma.Conclusion: The results showed a detection accuracy of protein in spiked plasma of >90% compared to PBS buffer, suggesting that the carboxyl-GO-based SPR biosensor could be used in assays of human plasma for early and late gynecological diseases. The future of this technology will be useful for the diagnosis and evaluation of the risk of early maternal preeclampsia and potentially in clinical applications for gestational diseases.Keywords: carboxyl, graphene oxide, carboxyl-GO, surface plasmon resonance, SPR, pregnancy, associated plasma protein A2, PAPPA2
format	article
author	Chiu NF Tai MJ Wu HP Lin TL Chen CY
author_facet	Chiu NF Tai MJ Wu HP Lin TL Chen CY
author_sort	Chiu NF
title	Development of a bioaffinity SPR immunosensor based on functionalized graphene oxide for the detection of pregnancy-associated plasma protein A2 in human plasma
title_short	Development of a bioaffinity SPR immunosensor based on functionalized graphene oxide for the detection of pregnancy-associated plasma protein A2 in human plasma
title_full	Development of a bioaffinity SPR immunosensor based on functionalized graphene oxide for the detection of pregnancy-associated plasma protein A2 in human plasma
title_fullStr	Development of a bioaffinity SPR immunosensor based on functionalized graphene oxide for the detection of pregnancy-associated plasma protein A2 in human plasma
title_full_unstemmed	Development of a bioaffinity SPR immunosensor based on functionalized graphene oxide for the detection of pregnancy-associated plasma protein A2 in human plasma
title_sort	development of a bioaffinity spr immunosensor based on functionalized graphene oxide for the detection of pregnancy-associated plasma protein a2 in human plasma
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/78437a5285a14d0baf4b1a3f38ea4a3f
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Development of a bioaffinity SPR immunosensor based on functionalized graphene oxide for the detection of pregnancy-associated plasma protein A2 in human plasma

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