Adherent and suspension baby hamster kidney cells have a different cytoskeleton and surface receptor repertoire.

Animal cell culture, with single cells growing in suspension, ideally in a chemically defined environment, is a mainstay of biopharmaceutical production. The synthetic environment lacks exogenous growth factors and usually requires a time-consuming adaptation process to select cell clones that proli...

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Autores principales:	Veronika Dill, Florian Pfaff, Aline Zimmer, Martin Beer, Michael Eschbaumer
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7844195279cd4641a6cc7bf885c17898
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spelling	oai:doaj.org-article:7844195279cd4641a6cc7bf885c178982021-12-02T20:03:55ZAdherent and suspension baby hamster kidney cells have a different cytoskeleton and surface receptor repertoire.1932-620310.1371/journal.pone.0246610https://doaj.org/article/7844195279cd4641a6cc7bf885c178982021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0246610https://doaj.org/toc/1932-6203Animal cell culture, with single cells growing in suspension, ideally in a chemically defined environment, is a mainstay of biopharmaceutical production. The synthetic environment lacks exogenous growth factors and usually requires a time-consuming adaptation process to select cell clones that proliferate in suspension to high cell numbers. The molecular mechanisms that facilitate the adaptation and that take place inside the cell are largely unknown. Especially for cell lines that are used for virus antigen production such as baby hamster kidney (BHK) cells, the restriction of virus growth through the evolution of undesired cell characteristics is highly unwanted. The comparison between adherently growing BHK cells and suspension cells with different susceptibility to foot-and-mouth disease virus revealed differences in the expression of cellular receptors such as integrins and heparan sulfates, and in the organization of the actin cytoskeleton. Transcriptome analyses and growth kinetics demonstrated the diversity of BHK cell lines and confirmed the importance of well-characterized parental cell clones and mindful screening to make sure that essential cellular features do not get lost during adaptation.Veronika DillFlorian PfaffAline ZimmerMartin BeerMichael EschbaumerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0246610 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Veronika Dill Florian Pfaff Aline Zimmer Martin Beer Michael Eschbaumer Adherent and suspension baby hamster kidney cells have a different cytoskeleton and surface receptor repertoire.
description	Animal cell culture, with single cells growing in suspension, ideally in a chemically defined environment, is a mainstay of biopharmaceutical production. The synthetic environment lacks exogenous growth factors and usually requires a time-consuming adaptation process to select cell clones that proliferate in suspension to high cell numbers. The molecular mechanisms that facilitate the adaptation and that take place inside the cell are largely unknown. Especially for cell lines that are used for virus antigen production such as baby hamster kidney (BHK) cells, the restriction of virus growth through the evolution of undesired cell characteristics is highly unwanted. The comparison between adherently growing BHK cells and suspension cells with different susceptibility to foot-and-mouth disease virus revealed differences in the expression of cellular receptors such as integrins and heparan sulfates, and in the organization of the actin cytoskeleton. Transcriptome analyses and growth kinetics demonstrated the diversity of BHK cell lines and confirmed the importance of well-characterized parental cell clones and mindful screening to make sure that essential cellular features do not get lost during adaptation.
format	article
author	Veronika Dill Florian Pfaff Aline Zimmer Martin Beer Michael Eschbaumer
author_facet	Veronika Dill Florian Pfaff Aline Zimmer Martin Beer Michael Eschbaumer
author_sort	Veronika Dill
title	Adherent and suspension baby hamster kidney cells have a different cytoskeleton and surface receptor repertoire.
title_short	Adherent and suspension baby hamster kidney cells have a different cytoskeleton and surface receptor repertoire.
title_full	Adherent and suspension baby hamster kidney cells have a different cytoskeleton and surface receptor repertoire.
title_fullStr	Adherent and suspension baby hamster kidney cells have a different cytoskeleton and surface receptor repertoire.
title_full_unstemmed	Adherent and suspension baby hamster kidney cells have a different cytoskeleton and surface receptor repertoire.
title_sort	adherent and suspension baby hamster kidney cells have a different cytoskeleton and surface receptor repertoire.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/7844195279cd4641a6cc7bf885c17898
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Adherent and suspension baby hamster kidney cells have a different cytoskeleton and surface receptor repertoire.

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