Dkk1 regulates ventral midbrain dopaminergic differentiation and morphogenesis.

Dickkopf1 (Dkk1) is a Wnt/β-catenin inhibitor that participates in many processes during embryonic development. One of its roles during embryogenesis is to induce head formation, since Dkk1-null mice lack head structures anterior to midbrain. The Wnt/β-catenin pathway is also known to regulate diffe...

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Autores principales:	Diogo Ribeiro, Kristina Ellwanger, Désirée Glagow, Spyridon Theofilopoulos, Nina S Corsini, Ana Martin-Villalba, Christof Niehrs, Ernest Arenas
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/785d671a481e489f97a8a2fdd6913b71
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spelling	oai:doaj.org-article:785d671a481e489f97a8a2fdd6913b712021-11-18T06:58:55ZDkk1 regulates ventral midbrain dopaminergic differentiation and morphogenesis.1932-620310.1371/journal.pone.0015786https://doaj.org/article/785d671a481e489f97a8a2fdd6913b712011-02-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21347250/?tool=EBIhttps://doaj.org/toc/1932-6203Dickkopf1 (Dkk1) is a Wnt/β-catenin inhibitor that participates in many processes during embryonic development. One of its roles during embryogenesis is to induce head formation, since Dkk1-null mice lack head structures anterior to midbrain. The Wnt/β-catenin pathway is also known to regulate different aspects of ventral midbrain (VM) dopaminergic (DA) neuron development and, in vitro, Dkk1-mediated inhibition of the Wnt/β-catenin pathway improves the DA differentiation in mouse embryonic stem cells (mESC). However, the in vivo function of Dkk1 on the development of midbrain DA neurons remains to be elucidated. Here we examined Dkk1(+/-) embryos and found that Dkk1 is required for the differentiation of DA precursors/neuroblasts into DA neurons at E13.5. This deficit persisted until E17.5, when a defect in the number and distribution of VM DA neurons was detected. Furthermore, analysis of the few Dkk1(-/-) embryos that survived until E17.5 revealed a more severe loss of midbrain DA neurons and morphogenesis defects. Our results thus show that Dkk1 is required for midbrain DA differentiation and morphogenesis.Diogo RibeiroKristina EllwangerDésirée GlagowSpyridon TheofilopoulosNina S CorsiniAna Martin-VillalbaChristof NiehrsErnest ArenasPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 2, p e15786 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Diogo Ribeiro Kristina Ellwanger Désirée Glagow Spyridon Theofilopoulos Nina S Corsini Ana Martin-Villalba Christof Niehrs Ernest Arenas Dkk1 regulates ventral midbrain dopaminergic differentiation and morphogenesis.
description	Dickkopf1 (Dkk1) is a Wnt/β-catenin inhibitor that participates in many processes during embryonic development. One of its roles during embryogenesis is to induce head formation, since Dkk1-null mice lack head structures anterior to midbrain. The Wnt/β-catenin pathway is also known to regulate different aspects of ventral midbrain (VM) dopaminergic (DA) neuron development and, in vitro, Dkk1-mediated inhibition of the Wnt/β-catenin pathway improves the DA differentiation in mouse embryonic stem cells (mESC). However, the in vivo function of Dkk1 on the development of midbrain DA neurons remains to be elucidated. Here we examined Dkk1(+/-) embryos and found that Dkk1 is required for the differentiation of DA precursors/neuroblasts into DA neurons at E13.5. This deficit persisted until E17.5, when a defect in the number and distribution of VM DA neurons was detected. Furthermore, analysis of the few Dkk1(-/-) embryos that survived until E17.5 revealed a more severe loss of midbrain DA neurons and morphogenesis defects. Our results thus show that Dkk1 is required for midbrain DA differentiation and morphogenesis.
format	article
author	Diogo Ribeiro Kristina Ellwanger Désirée Glagow Spyridon Theofilopoulos Nina S Corsini Ana Martin-Villalba Christof Niehrs Ernest Arenas
author_facet	Diogo Ribeiro Kristina Ellwanger Désirée Glagow Spyridon Theofilopoulos Nina S Corsini Ana Martin-Villalba Christof Niehrs Ernest Arenas
author_sort	Diogo Ribeiro
title	Dkk1 regulates ventral midbrain dopaminergic differentiation and morphogenesis.
title_short	Dkk1 regulates ventral midbrain dopaminergic differentiation and morphogenesis.
title_full	Dkk1 regulates ventral midbrain dopaminergic differentiation and morphogenesis.
title_fullStr	Dkk1 regulates ventral midbrain dopaminergic differentiation and morphogenesis.
title_full_unstemmed	Dkk1 regulates ventral midbrain dopaminergic differentiation and morphogenesis.
title_sort	dkk1 regulates ventral midbrain dopaminergic differentiation and morphogenesis.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/785d671a481e489f97a8a2fdd6913b71
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_version_	1718424111714140160

Dkk1 regulates ventral midbrain dopaminergic differentiation and morphogenesis.

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