Optimization of ClpXP activity and protein synthesis in an E. coli extract-based cell-free expression system

Abstract Protein degradation is a fundamental process in all living cells and is essential to remove both damaged proteins and intact proteins that are no longer needed by the cell. We are interested in creating synthetic genetic circuits that function in a cell-free expression system. This will req...

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Autores principales:	Xinying Shi, Ti Wu, Christian M. Cole, Neal K. Devaraj, Simpson Joseph
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7864bd636f484af58fd311bfaf3f0ba4
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spelling	oai:doaj.org-article:7864bd636f484af58fd311bfaf3f0ba42021-12-02T12:32:10ZOptimization of ClpXP activity and protein synthesis in an E. coli extract-based cell-free expression system10.1038/s41598-018-21739-62045-2322https://doaj.org/article/7864bd636f484af58fd311bfaf3f0ba42018-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-21739-6https://doaj.org/toc/2045-2322Abstract Protein degradation is a fundamental process in all living cells and is essential to remove both damaged proteins and intact proteins that are no longer needed by the cell. We are interested in creating synthetic genetic circuits that function in a cell-free expression system. This will require not only an efficient protein expression platform but also a robust protein degradation system in cell extract. Therefore, we purified and tested the activity of E. coli ClpXP protease in cell-free transcription-translation (TX-TL) systems that used E. coli S30 cell extract. Surprisingly, our studies showed that purified ClpXP added to the TX-TL system has very low proteolytic activity. The low activity of ClpXP was correlated with the rapid consumption of adenosine triphosphate (ATP) in cell extract. We improved the activity of ClpXP in cell extract by adding exogenous ATP and an energy regeneration system. We then established conditions for both protein synthesis, and protein degradation by ClpXP to occur simultaneously in the TX-TL systems. The optimized conditions for ClpXP activity will be useful for creating tunable synthetic genetic circuits and in vitro synthetic biology.Xinying ShiTi WuChristian M. ColeNeal K. DevarajSimpson JosephNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xinying Shi Ti Wu Christian M. Cole Neal K. Devaraj Simpson Joseph Optimization of ClpXP activity and protein synthesis in an E. coli extract-based cell-free expression system
description	Abstract Protein degradation is a fundamental process in all living cells and is essential to remove both damaged proteins and intact proteins that are no longer needed by the cell. We are interested in creating synthetic genetic circuits that function in a cell-free expression system. This will require not only an efficient protein expression platform but also a robust protein degradation system in cell extract. Therefore, we purified and tested the activity of E. coli ClpXP protease in cell-free transcription-translation (TX-TL) systems that used E. coli S30 cell extract. Surprisingly, our studies showed that purified ClpXP added to the TX-TL system has very low proteolytic activity. The low activity of ClpXP was correlated with the rapid consumption of adenosine triphosphate (ATP) in cell extract. We improved the activity of ClpXP in cell extract by adding exogenous ATP and an energy regeneration system. We then established conditions for both protein synthesis, and protein degradation by ClpXP to occur simultaneously in the TX-TL systems. The optimized conditions for ClpXP activity will be useful for creating tunable synthetic genetic circuits and in vitro synthetic biology.
format	article
author	Xinying Shi Ti Wu Christian M. Cole Neal K. Devaraj Simpson Joseph
author_facet	Xinying Shi Ti Wu Christian M. Cole Neal K. Devaraj Simpson Joseph
author_sort	Xinying Shi
title	Optimization of ClpXP activity and protein synthesis in an E. coli extract-based cell-free expression system
title_short	Optimization of ClpXP activity and protein synthesis in an E. coli extract-based cell-free expression system
title_full	Optimization of ClpXP activity and protein synthesis in an E. coli extract-based cell-free expression system
title_fullStr	Optimization of ClpXP activity and protein synthesis in an E. coli extract-based cell-free expression system
title_full_unstemmed	Optimization of ClpXP activity and protein synthesis in an E. coli extract-based cell-free expression system
title_sort	optimization of clpxp activity and protein synthesis in an e. coli extract-based cell-free expression system
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/7864bd636f484af58fd311bfaf3f0ba4
work_keys_str_mv	AT xinyingshi optimizationofclpxpactivityandproteinsynthesisinanecoliextractbasedcellfreeexpressionsystem AT tiwu optimizationofclpxpactivityandproteinsynthesisinanecoliextractbasedcellfreeexpressionsystem AT christianmcole optimizationofclpxpactivityandproteinsynthesisinanecoliextractbasedcellfreeexpressionsystem AT nealkdevaraj optimizationofclpxpactivityandproteinsynthesisinanecoliextractbasedcellfreeexpressionsystem AT simpsonjoseph optimizationofclpxpactivityandproteinsynthesisinanecoliextractbasedcellfreeexpressionsystem
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Optimization of ClpXP activity and protein synthesis in an E. coli extract-based cell-free expression system

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