Development of a robust protocol for the characterization of the pulmonary microbiota

Nathan Dumont-Leblond et al. present a protocol for lung microbiota analysis, including all steps from patient recruitment to bioinformatics. The data show how methodological variation, such as use of different DNA extraction kits, can impact the results and represent an important step toward method...

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Detalles Bibliográficos
Autores principales:	Nathan Dumont-Leblond, Marc Veillette, Christine Racine, Philippe Joubert, Caroline Duchaine
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/78707ce9180c4e8fb05eee1c91060e35
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spelling	oai:doaj.org-article:78707ce9180c4e8fb05eee1c91060e352021-12-02T14:06:19ZDevelopment of a robust protocol for the characterization of the pulmonary microbiota10.1038/s42003-021-01690-52399-3642https://doaj.org/article/78707ce9180c4e8fb05eee1c91060e352021-02-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-01690-5https://doaj.org/toc/2399-3642Nathan Dumont-Leblond et al. present a protocol for lung microbiota analysis, including all steps from patient recruitment to bioinformatics. The data show how methodological variation, such as use of different DNA extraction kits, can impact the results and represent an important step toward methods standardization in the pulmonary microbiome field.Nathan Dumont-LeblondMarc VeilletteChristine RacinePhilippe JoubertCaroline DuchaineNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Nathan Dumont-Leblond Marc Veillette Christine Racine Philippe Joubert Caroline Duchaine Development of a robust protocol for the characterization of the pulmonary microbiota
description	Nathan Dumont-Leblond et al. present a protocol for lung microbiota analysis, including all steps from patient recruitment to bioinformatics. The data show how methodological variation, such as use of different DNA extraction kits, can impact the results and represent an important step toward methods standardization in the pulmonary microbiome field.
format	article
author	Nathan Dumont-Leblond Marc Veillette Christine Racine Philippe Joubert Caroline Duchaine
author_facet	Nathan Dumont-Leblond Marc Veillette Christine Racine Philippe Joubert Caroline Duchaine
author_sort	Nathan Dumont-Leblond
title	Development of a robust protocol for the characterization of the pulmonary microbiota
title_short	Development of a robust protocol for the characterization of the pulmonary microbiota
title_full	Development of a robust protocol for the characterization of the pulmonary microbiota
title_fullStr	Development of a robust protocol for the characterization of the pulmonary microbiota
title_full_unstemmed	Development of a robust protocol for the characterization of the pulmonary microbiota
title_sort	development of a robust protocol for the characterization of the pulmonary microbiota
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/78707ce9180c4e8fb05eee1c91060e35
work_keys_str_mv	AT nathandumontleblond developmentofarobustprotocolforthecharacterizationofthepulmonarymicrobiota AT marcveillette developmentofarobustprotocolforthecharacterizationofthepulmonarymicrobiota AT christineracine developmentofarobustprotocolforthecharacterizationofthepulmonarymicrobiota AT philippejoubert developmentofarobustprotocolforthecharacterizationofthepulmonarymicrobiota AT carolineduchaine developmentofarobustprotocolforthecharacterizationofthepulmonarymicrobiota
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Development of a robust protocol for the characterization of the pulmonary microbiota

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