High-throughput 5′ UTR engineering for enhanced protein production in non-viral gene therapies

The engineering of 5′ UTRs that modulate protein expression remains a great challenge. Here we leverage synthetic biology and computational design to develop a high-throughput strategy to design, screen, and optimize 5′ UTRs that enhance protein expression for non-viral gene therapies.

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Autores principales:	Jicong Cao, Eva Maria Novoa, Zhizhuo Zhang, William C. W. Chen, Dianbo Liu, Gigi C. G. Choi, Alan S. L. Wong, Claudia Wehrspaun, Manolis Kellis, Timothy K. Lu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/7874f9ea0bf346329b2ba9f6b304ff50
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spelling	oai:doaj.org-article:7874f9ea0bf346329b2ba9f6b304ff502021-12-02T16:14:58ZHigh-throughput 5′ UTR engineering for enhanced protein production in non-viral gene therapies10.1038/s41467-021-24436-72041-1723https://doaj.org/article/7874f9ea0bf346329b2ba9f6b304ff502021-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-24436-7https://doaj.org/toc/2041-1723The engineering of 5′ UTRs that modulate protein expression remains a great challenge. Here we leverage synthetic biology and computational design to develop a high-throughput strategy to design, screen, and optimize 5′ UTRs that enhance protein expression for non-viral gene therapies.Jicong CaoEva Maria NovoaZhizhuo ZhangWilliam C. W. ChenDianbo LiuGigi C. G. ChoiAlan S. L. WongClaudia WehrspaunManolis KellisTimothy K. LuNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Jicong Cao Eva Maria Novoa Zhizhuo Zhang William C. W. Chen Dianbo Liu Gigi C. G. Choi Alan S. L. Wong Claudia Wehrspaun Manolis Kellis Timothy K. Lu High-throughput 5′ UTR engineering for enhanced protein production in non-viral gene therapies
description	The engineering of 5′ UTRs that modulate protein expression remains a great challenge. Here we leverage synthetic biology and computational design to develop a high-throughput strategy to design, screen, and optimize 5′ UTRs that enhance protein expression for non-viral gene therapies.
format	article
author	Jicong Cao Eva Maria Novoa Zhizhuo Zhang William C. W. Chen Dianbo Liu Gigi C. G. Choi Alan S. L. Wong Claudia Wehrspaun Manolis Kellis Timothy K. Lu
author_facet	Jicong Cao Eva Maria Novoa Zhizhuo Zhang William C. W. Chen Dianbo Liu Gigi C. G. Choi Alan S. L. Wong Claudia Wehrspaun Manolis Kellis Timothy K. Lu
author_sort	Jicong Cao
title	High-throughput 5′ UTR engineering for enhanced protein production in non-viral gene therapies
title_short	High-throughput 5′ UTR engineering for enhanced protein production in non-viral gene therapies
title_full	High-throughput 5′ UTR engineering for enhanced protein production in non-viral gene therapies
title_fullStr	High-throughput 5′ UTR engineering for enhanced protein production in non-viral gene therapies
title_full_unstemmed	High-throughput 5′ UTR engineering for enhanced protein production in non-viral gene therapies
title_sort	high-throughput 5′ utr engineering for enhanced protein production in non-viral gene therapies
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7874f9ea0bf346329b2ba9f6b304ff50
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High-throughput 5′ UTR engineering for enhanced protein production in non-viral gene therapies

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