Development of an antibody fragment that stabilizes GPCR/G-protein complexes

The determination of high resolution structures of G protein coupled receptors (GPCRs) in complex with heterotrimeric G proteins is challenging. Here authors develop an antibody fragment, mAB16, which stabilizes GPCR/G-protein complexes and facilitates the application of high resolution cryo-EM.

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Autores principales:	Shoji Maeda, Antoine Koehl, Hugues Matile, Hongli Hu, Daniel Hilger, Gebhard F. X. Schertler, Aashish Manglik, Georgios Skiniotis, Roger J. P. Dawson, Brian K. Kobilka
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/78767849a46d4536a969df36dd817784
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spelling	oai:doaj.org-article:78767849a46d4536a969df36dd8177842021-12-02T17:31:45ZDevelopment of an antibody fragment that stabilizes GPCR/G-protein complexes10.1038/s41467-018-06002-w2041-1723https://doaj.org/article/78767849a46d4536a969df36dd8177842018-09-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-06002-whttps://doaj.org/toc/2041-1723The determination of high resolution structures of G protein coupled receptors (GPCRs) in complex with heterotrimeric G proteins is challenging. Here authors develop an antibody fragment, mAB16, which stabilizes GPCR/G-protein complexes and facilitates the application of high resolution cryo-EM.Shoji MaedaAntoine KoehlHugues MatileHongli HuDaniel HilgerGebhard F. X. SchertlerAashish ManglikGeorgios SkiniotisRoger J. P. DawsonBrian K. KobilkaNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-9 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Shoji Maeda Antoine Koehl Hugues Matile Hongli Hu Daniel Hilger Gebhard F. X. Schertler Aashish Manglik Georgios Skiniotis Roger J. P. Dawson Brian K. Kobilka Development of an antibody fragment that stabilizes GPCR/G-protein complexes
description	The determination of high resolution structures of G protein coupled receptors (GPCRs) in complex with heterotrimeric G proteins is challenging. Here authors develop an antibody fragment, mAB16, which stabilizes GPCR/G-protein complexes and facilitates the application of high resolution cryo-EM.
format	article
author	Shoji Maeda Antoine Koehl Hugues Matile Hongli Hu Daniel Hilger Gebhard F. X. Schertler Aashish Manglik Georgios Skiniotis Roger J. P. Dawson Brian K. Kobilka
author_facet	Shoji Maeda Antoine Koehl Hugues Matile Hongli Hu Daniel Hilger Gebhard F. X. Schertler Aashish Manglik Georgios Skiniotis Roger J. P. Dawson Brian K. Kobilka
author_sort	Shoji Maeda
title	Development of an antibody fragment that stabilizes GPCR/G-protein complexes
title_short	Development of an antibody fragment that stabilizes GPCR/G-protein complexes
title_full	Development of an antibody fragment that stabilizes GPCR/G-protein complexes
title_fullStr	Development of an antibody fragment that stabilizes GPCR/G-protein complexes
title_full_unstemmed	Development of an antibody fragment that stabilizes GPCR/G-protein complexes
title_sort	development of an antibody fragment that stabilizes gpcr/g-protein complexes
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/78767849a46d4536a969df36dd817784
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Development of an antibody fragment that stabilizes GPCR/G-protein complexes

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