Kinetic and structural mechanism for DNA unwinding by a non-hexameric helicase

UvrD is a model helicase from the non-hexameric Superfamily 1. Here, the authors use optical tweezers to measure directly the stepwise translocation of UvrD along a DNA hairpin, and propose a mechanism in which UvrD moves one base pair at a time, but sequesters the nascent single strands, releasing...

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Autores principales:	Sean P. Carney, Wen Ma, Kevin D. Whitley, Haifeng Jia, Timothy M. Lohman, Zaida Luthey-Schulten, Yann R. Chemla
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/787da31e7c364e8b9f5fc0478dc6983b
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spelling	oai:doaj.org-article:787da31e7c364e8b9f5fc0478dc6983b2021-12-05T12:23:01ZKinetic and structural mechanism for DNA unwinding by a non-hexameric helicase10.1038/s41467-021-27304-62041-1723https://doaj.org/article/787da31e7c364e8b9f5fc0478dc6983b2021-12-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-27304-6https://doaj.org/toc/2041-1723UvrD is a model helicase from the non-hexameric Superfamily 1. Here, the authors use optical tweezers to measure directly the stepwise translocation of UvrD along a DNA hairpin, and propose a mechanism in which UvrD moves one base pair at a time, but sequesters the nascent single strands, releasing them after a variable number of ATP hydrolysis cycles.Sean P. CarneyWen MaKevin D. WhitleyHaifeng JiaTimothy M. LohmanZaida Luthey-SchultenYann R. ChemlaNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Sean P. Carney Wen Ma Kevin D. Whitley Haifeng Jia Timothy M. Lohman Zaida Luthey-Schulten Yann R. Chemla Kinetic and structural mechanism for DNA unwinding by a non-hexameric helicase
description	UvrD is a model helicase from the non-hexameric Superfamily 1. Here, the authors use optical tweezers to measure directly the stepwise translocation of UvrD along a DNA hairpin, and propose a mechanism in which UvrD moves one base pair at a time, but sequesters the nascent single strands, releasing them after a variable number of ATP hydrolysis cycles.
format	article
author	Sean P. Carney Wen Ma Kevin D. Whitley Haifeng Jia Timothy M. Lohman Zaida Luthey-Schulten Yann R. Chemla
author_facet	Sean P. Carney Wen Ma Kevin D. Whitley Haifeng Jia Timothy M. Lohman Zaida Luthey-Schulten Yann R. Chemla
author_sort	Sean P. Carney
title	Kinetic and structural mechanism for DNA unwinding by a non-hexameric helicase
title_short	Kinetic and structural mechanism for DNA unwinding by a non-hexameric helicase
title_full	Kinetic and structural mechanism for DNA unwinding by a non-hexameric helicase
title_fullStr	Kinetic and structural mechanism for DNA unwinding by a non-hexameric helicase
title_full_unstemmed	Kinetic and structural mechanism for DNA unwinding by a non-hexameric helicase
title_sort	kinetic and structural mechanism for dna unwinding by a non-hexameric helicase
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/787da31e7c364e8b9f5fc0478dc6983b
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Kinetic and structural mechanism for DNA unwinding by a non-hexameric helicase

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