Revealing region-specific biofilm viscoelastic properties by means of a micro-rheological approach

Analytics: A better look at biofilms Combining two distinct techniques allows analysis of the structure and physical properties of bacterial biofilms in fine detail. Eoin Casey and colleagues, from University College Dublin, Ireland, and the University of Hong Kong, applied a combined non-destructiv...

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Autores principales: Huayu Cao, Olivier Habimana, Ashkan Safari, Rory Heffernan, Yihong Dai, Eoin Casey
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2016
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Acceso en línea:https://doaj.org/article/7888515461c043a280fd9320b8e4854f
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spelling oai:doaj.org-article:7888515461c043a280fd9320b8e4854f2021-12-02T16:09:01ZRevealing region-specific biofilm viscoelastic properties by means of a micro-rheological approach10.1038/s41522-016-0005-y2055-5008https://doaj.org/article/7888515461c043a280fd9320b8e4854f2016-12-01T00:00:00Zhttps://doi.org/10.1038/s41522-016-0005-yhttps://doaj.org/toc/2055-5008Analytics: A better look at biofilms Combining two distinct techniques allows analysis of the structure and physical properties of bacterial biofilms in fine detail. Eoin Casey and colleagues, from University College Dublin, Ireland, and the University of Hong Kong, applied a combined non-destructive procedure to cultured biofilms, for determining biofilm slimy properties and structural features. Tracking the movement of tiny fluorescent beads embedded in the biofilms yielded insights into their localised material properties. The same samples were also analysed using a laser-based imaging technique called confocal microscopy, which added additional structural insight to the biofilm samples. This combination allowed an improved regional understanding of the biofilms’ varying material and mechanical properties, including viscosity and elasticity. In future, the researchers hope to add chemical analysis into the mix, producing a method to search for better ways to treat and eradicate biofilms.Huayu CaoOlivier HabimanaAshkan SafariRory HeffernanYihong DaiEoin CaseyNature PortfolioarticleMicrobial ecologyQR100-130ENnpj Biofilms and Microbiomes, Vol 2, Iss 1, Pp 1-7 (2016)
institution DOAJ
collection DOAJ
language EN
topic Microbial ecology
QR100-130
spellingShingle Microbial ecology
QR100-130
Huayu Cao
Olivier Habimana
Ashkan Safari
Rory Heffernan
Yihong Dai
Eoin Casey
Revealing region-specific biofilm viscoelastic properties by means of a micro-rheological approach
description Analytics: A better look at biofilms Combining two distinct techniques allows analysis of the structure and physical properties of bacterial biofilms in fine detail. Eoin Casey and colleagues, from University College Dublin, Ireland, and the University of Hong Kong, applied a combined non-destructive procedure to cultured biofilms, for determining biofilm slimy properties and structural features. Tracking the movement of tiny fluorescent beads embedded in the biofilms yielded insights into their localised material properties. The same samples were also analysed using a laser-based imaging technique called confocal microscopy, which added additional structural insight to the biofilm samples. This combination allowed an improved regional understanding of the biofilms’ varying material and mechanical properties, including viscosity and elasticity. In future, the researchers hope to add chemical analysis into the mix, producing a method to search for better ways to treat and eradicate biofilms.
format article
author Huayu Cao
Olivier Habimana
Ashkan Safari
Rory Heffernan
Yihong Dai
Eoin Casey
author_facet Huayu Cao
Olivier Habimana
Ashkan Safari
Rory Heffernan
Yihong Dai
Eoin Casey
author_sort Huayu Cao
title Revealing region-specific biofilm viscoelastic properties by means of a micro-rheological approach
title_short Revealing region-specific biofilm viscoelastic properties by means of a micro-rheological approach
title_full Revealing region-specific biofilm viscoelastic properties by means of a micro-rheological approach
title_fullStr Revealing region-specific biofilm viscoelastic properties by means of a micro-rheological approach
title_full_unstemmed Revealing region-specific biofilm viscoelastic properties by means of a micro-rheological approach
title_sort revealing region-specific biofilm viscoelastic properties by means of a micro-rheological approach
publisher Nature Portfolio
publishDate 2016
url https://doaj.org/article/7888515461c043a280fd9320b8e4854f
work_keys_str_mv AT huayucao revealingregionspecificbiofilmviscoelasticpropertiesbymeansofamicrorheologicalapproach
AT olivierhabimana revealingregionspecificbiofilmviscoelasticpropertiesbymeansofamicrorheologicalapproach
AT ashkansafari revealingregionspecificbiofilmviscoelasticpropertiesbymeansofamicrorheologicalapproach
AT roryheffernan revealingregionspecificbiofilmviscoelasticpropertiesbymeansofamicrorheologicalapproach
AT yihongdai revealingregionspecificbiofilmviscoelasticpropertiesbymeansofamicrorheologicalapproach
AT eoincasey revealingregionspecificbiofilmviscoelasticpropertiesbymeansofamicrorheologicalapproach
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