Revealing region-specific biofilm viscoelastic properties by means of a micro-rheological approach
Analytics: A better look at biofilms Combining two distinct techniques allows analysis of the structure and physical properties of bacterial biofilms in fine detail. Eoin Casey and colleagues, from University College Dublin, Ireland, and the University of Hong Kong, applied a combined non-destructiv...
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Nature Portfolio
2016
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oai:doaj.org-article:7888515461c043a280fd9320b8e4854f2021-12-02T16:09:01ZRevealing region-specific biofilm viscoelastic properties by means of a micro-rheological approach10.1038/s41522-016-0005-y2055-5008https://doaj.org/article/7888515461c043a280fd9320b8e4854f2016-12-01T00:00:00Zhttps://doi.org/10.1038/s41522-016-0005-yhttps://doaj.org/toc/2055-5008Analytics: A better look at biofilms Combining two distinct techniques allows analysis of the structure and physical properties of bacterial biofilms in fine detail. Eoin Casey and colleagues, from University College Dublin, Ireland, and the University of Hong Kong, applied a combined non-destructive procedure to cultured biofilms, for determining biofilm slimy properties and structural features. Tracking the movement of tiny fluorescent beads embedded in the biofilms yielded insights into their localised material properties. The same samples were also analysed using a laser-based imaging technique called confocal microscopy, which added additional structural insight to the biofilm samples. This combination allowed an improved regional understanding of the biofilms’ varying material and mechanical properties, including viscosity and elasticity. In future, the researchers hope to add chemical analysis into the mix, producing a method to search for better ways to treat and eradicate biofilms.Huayu CaoOlivier HabimanaAshkan SafariRory HeffernanYihong DaiEoin CaseyNature PortfolioarticleMicrobial ecologyQR100-130ENnpj Biofilms and Microbiomes, Vol 2, Iss 1, Pp 1-7 (2016) |
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Microbial ecology QR100-130 |
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Microbial ecology QR100-130 Huayu Cao Olivier Habimana Ashkan Safari Rory Heffernan Yihong Dai Eoin Casey Revealing region-specific biofilm viscoelastic properties by means of a micro-rheological approach |
description |
Analytics: A better look at biofilms Combining two distinct techniques allows analysis of the structure and physical properties of bacterial biofilms in fine detail. Eoin Casey and colleagues, from University College Dublin, Ireland, and the University of Hong Kong, applied a combined non-destructive procedure to cultured biofilms, for determining biofilm slimy properties and structural features. Tracking the movement of tiny fluorescent beads embedded in the biofilms yielded insights into their localised material properties. The same samples were also analysed using a laser-based imaging technique called confocal microscopy, which added additional structural insight to the biofilm samples. This combination allowed an improved regional understanding of the biofilms’ varying material and mechanical properties, including viscosity and elasticity. In future, the researchers hope to add chemical analysis into the mix, producing a method to search for better ways to treat and eradicate biofilms. |
format |
article |
author |
Huayu Cao Olivier Habimana Ashkan Safari Rory Heffernan Yihong Dai Eoin Casey |
author_facet |
Huayu Cao Olivier Habimana Ashkan Safari Rory Heffernan Yihong Dai Eoin Casey |
author_sort |
Huayu Cao |
title |
Revealing region-specific biofilm viscoelastic properties by means of a micro-rheological approach |
title_short |
Revealing region-specific biofilm viscoelastic properties by means of a micro-rheological approach |
title_full |
Revealing region-specific biofilm viscoelastic properties by means of a micro-rheological approach |
title_fullStr |
Revealing region-specific biofilm viscoelastic properties by means of a micro-rheological approach |
title_full_unstemmed |
Revealing region-specific biofilm viscoelastic properties by means of a micro-rheological approach |
title_sort |
revealing region-specific biofilm viscoelastic properties by means of a micro-rheological approach |
publisher |
Nature Portfolio |
publishDate |
2016 |
url |
https://doaj.org/article/7888515461c043a280fd9320b8e4854f |
work_keys_str_mv |
AT huayucao revealingregionspecificbiofilmviscoelasticpropertiesbymeansofamicrorheologicalapproach AT olivierhabimana revealingregionspecificbiofilmviscoelasticpropertiesbymeansofamicrorheologicalapproach AT ashkansafari revealingregionspecificbiofilmviscoelasticpropertiesbymeansofamicrorheologicalapproach AT roryheffernan revealingregionspecificbiofilmviscoelasticpropertiesbymeansofamicrorheologicalapproach AT yihongdai revealingregionspecificbiofilmviscoelasticpropertiesbymeansofamicrorheologicalapproach AT eoincasey revealingregionspecificbiofilmviscoelasticpropertiesbymeansofamicrorheologicalapproach |
_version_ |
1718384477535731712 |