Analysis of Bimatoprost-Induced changes on Rabbits eyelash Follicle: Clinical and Electron microscopic study

Khulood Muhammad Sayed,1 Engy Mohammed Mostafa,1 Amr Mounir,1 Nesreen G Abd Elhaliem,2 Alahmady Hamad Alsmman1 1The Department of Ophthalmology, Sohag Faculty of Medicine, Sohag University, Sohag, Egypt; 2The Department of Histology, Sohag Faculty of Medicine, Sohag University, Sohag, EgyptCorrespon...

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Autores principales:	Sayed KM, Mostafa EM, Mounir A, Abd Elhaliem NG, Alsmman AH
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	bimatoprost rabbit eyelash hair follicle Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/7888718823384b959f9955cbd76ddb34
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spelling	oai:doaj.org-article:7888718823384b959f9955cbd76ddb342021-12-02T02:39:12ZAnalysis of Bimatoprost-Induced changes on Rabbits eyelash Follicle: Clinical and Electron microscopic study1177-5483https://doaj.org/article/7888718823384b959f9955cbd76ddb342019-12-01T00:00:00Zhttps://www.dovepress.com/analysis-of-bimatoprost-induced-changes-on-rabbits-eyelash-follicle-cl-peer-reviewed-article-OPTHhttps://doaj.org/toc/1177-5483Khulood Muhammad Sayed,1 Engy Mohammed Mostafa,1 Amr Mounir,1 Nesreen G Abd Elhaliem,2 Alahmady Hamad Alsmman1 1The Department of Ophthalmology, Sohag Faculty of Medicine, Sohag University, Sohag, Egypt; 2The Department of Histology, Sohag Faculty of Medicine, Sohag University, Sohag, EgyptCorrespondence: Alahmady Hamad AlsmmanThe Department of Ophthalmology, Sohag Faculty of Medicine, Sohag University, Sohag 82524, EgyptTel +201111102698Email alahmady20@yahoo.comObjective: To analyze ultrastructural changes of rabbits’ eyelash follicles treated with bimatoprost eye drops to increase our knowledge of how this drug works.Methods: The study included 15 clinically healthy male rabbits. All rabbits were treated with bimatoprost 0.03% daily for 4 weeks with one drop of the topical eye drops applied to the conjunctival fornix of the right eyes; left eyes were used as controls. Eyelash lengths were measured before and after treatment. The eyelid of each animal was dissected for light and electron microscopic analysis.Results: Both control and treated rabbit eyes matched regarding eyelash length before treatment (9.80±0.388mm vs 9.88±0.24mm) (P=0.108). There was a significant increase in eyelash length between control (9.75±0.33 mm) and treated rabbit eyes (11.60±0.46 mm) (P=0.369). Light and electron microscopy revealed, bimatoprost treated eyes had thick epidermis. The dermis contained two hairs growing out of the same hair follicle. Heavily keratinized Henle’s layer, the cortical cells (Cx) have prominent nucleolus and cytoplasm is studded with melanin granules.Conclusion: Bimatoprost-induced eyelash changes were not restricted to increased eyelash length, thickness, and pigmentation but also showed increased number of eyelashes within the same hair follicle which were stronger and could resist pulling from the skin without any evidence of inflammatory cells within the specimens. These changes occurred as early as 1 month of treatment, giving rise to thoughts about the possibility of using bimatoprost eye drops as a prophylaxis against madarosis associated with chemotherapy if it is started 1 month before chemotherapy and continued afterwards, making eyelashes stronger and resistant to falling out.Keywords: bimatoprost, rabbit, eyelash, hair follicleSayed KMMostafa EMMounir AAbd Elhaliem NGAlsmman AHDove Medical Pressarticlebimatoprostrabbiteyelashhair follicleOphthalmologyRE1-994ENClinical Ophthalmology, Vol Volume 13, Pp 2421-2426 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	bimatoprost rabbit eyelash hair follicle Ophthalmology RE1-994
spellingShingle	bimatoprost rabbit eyelash hair follicle Ophthalmology RE1-994 Sayed KM Mostafa EM Mounir A Abd Elhaliem NG Alsmman AH Analysis of Bimatoprost-Induced changes on Rabbits eyelash Follicle: Clinical and Electron microscopic study
description	Khulood Muhammad Sayed,1 Engy Mohammed Mostafa,1 Amr Mounir,1 Nesreen G Abd Elhaliem,2 Alahmady Hamad Alsmman1 1The Department of Ophthalmology, Sohag Faculty of Medicine, Sohag University, Sohag, Egypt; 2The Department of Histology, Sohag Faculty of Medicine, Sohag University, Sohag, EgyptCorrespondence: Alahmady Hamad AlsmmanThe Department of Ophthalmology, Sohag Faculty of Medicine, Sohag University, Sohag 82524, EgyptTel +201111102698Email alahmady20@yahoo.comObjective: To analyze ultrastructural changes of rabbits’ eyelash follicles treated with bimatoprost eye drops to increase our knowledge of how this drug works.Methods: The study included 15 clinically healthy male rabbits. All rabbits were treated with bimatoprost 0.03% daily for 4 weeks with one drop of the topical eye drops applied to the conjunctival fornix of the right eyes; left eyes were used as controls. Eyelash lengths were measured before and after treatment. The eyelid of each animal was dissected for light and electron microscopic analysis.Results: Both control and treated rabbit eyes matched regarding eyelash length before treatment (9.80±0.388mm vs 9.88±0.24mm) (P=0.108). There was a significant increase in eyelash length between control (9.75±0.33 mm) and treated rabbit eyes (11.60±0.46 mm) (P=0.369). Light and electron microscopy revealed, bimatoprost treated eyes had thick epidermis. The dermis contained two hairs growing out of the same hair follicle. Heavily keratinized Henle’s layer, the cortical cells (Cx) have prominent nucleolus and cytoplasm is studded with melanin granules.Conclusion: Bimatoprost-induced eyelash changes were not restricted to increased eyelash length, thickness, and pigmentation but also showed increased number of eyelashes within the same hair follicle which were stronger and could resist pulling from the skin without any evidence of inflammatory cells within the specimens. These changes occurred as early as 1 month of treatment, giving rise to thoughts about the possibility of using bimatoprost eye drops as a prophylaxis against madarosis associated with chemotherapy if it is started 1 month before chemotherapy and continued afterwards, making eyelashes stronger and resistant to falling out.Keywords: bimatoprost, rabbit, eyelash, hair follicle
format	article
author	Sayed KM Mostafa EM Mounir A Abd Elhaliem NG Alsmman AH
author_facet	Sayed KM Mostafa EM Mounir A Abd Elhaliem NG Alsmman AH
author_sort	Sayed KM
title	Analysis of Bimatoprost-Induced changes on Rabbits eyelash Follicle: Clinical and Electron microscopic study
title_short	Analysis of Bimatoprost-Induced changes on Rabbits eyelash Follicle: Clinical and Electron microscopic study
title_full	Analysis of Bimatoprost-Induced changes on Rabbits eyelash Follicle: Clinical and Electron microscopic study
title_fullStr	Analysis of Bimatoprost-Induced changes on Rabbits eyelash Follicle: Clinical and Electron microscopic study
title_full_unstemmed	Analysis of Bimatoprost-Induced changes on Rabbits eyelash Follicle: Clinical and Electron microscopic study
title_sort	analysis of bimatoprost-induced changes on rabbits eyelash follicle: clinical and electron microscopic study
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/7888718823384b959f9955cbd76ddb34
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Analysis of Bimatoprost-Induced changes on Rabbits eyelash Follicle: Clinical and Electron microscopic study

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