Spy&Go purification of SpyTag-proteins using pseudo-SpyCatcher to access an oligomerization toolbox

Peptide tags are important for protein purification but have minimal benefits afterwards. Here the authors present Spy&Go, which uses rational engineering of the SpyDock protein to capture SpyTag peptides without requiring an irreverisble isopeptide bond.

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Bibliographic Details
Main Authors:	Irsyad N. A. Khairil Anuar, Anusuya Banerjee, Anthony H. Keeble, Alberto Carella, Georgi I. Nikov, Mark Howarth
Format:	article
Language:	EN
Published:	Nature Portfolio 2019
Subjects:	Science Q
Online Access:	https://doaj.org/article/7888920400c8448ebf0e86fe447eb9a5
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id	oai:doaj.org-article:7888920400c8448ebf0e86fe447eb9a5
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spelling	oai:doaj.org-article:7888920400c8448ebf0e86fe447eb9a52021-12-02T14:40:17ZSpy&Go purification of SpyTag-proteins using pseudo-SpyCatcher to access an oligomerization toolbox10.1038/s41467-019-09678-w2041-1723https://doaj.org/article/7888920400c8448ebf0e86fe447eb9a52019-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-09678-whttps://doaj.org/toc/2041-1723Peptide tags are important for protein purification but have minimal benefits afterwards. Here the authors present Spy&Go, which uses rational engineering of the SpyDock protein to capture SpyTag peptides without requiring an irreverisble isopeptide bond.Irsyad N. A. Khairil AnuarAnusuya BanerjeeAnthony H. KeebleAlberto CarellaGeorgi I. NikovMark HowarthNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-13 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Irsyad N. A. Khairil Anuar Anusuya Banerjee Anthony H. Keeble Alberto Carella Georgi I. Nikov Mark Howarth Spy&Go purification of SpyTag-proteins using pseudo-SpyCatcher to access an oligomerization toolbox
description	Peptide tags are important for protein purification but have minimal benefits afterwards. Here the authors present Spy&Go, which uses rational engineering of the SpyDock protein to capture SpyTag peptides without requiring an irreverisble isopeptide bond.
format	article
author	Irsyad N. A. Khairil Anuar Anusuya Banerjee Anthony H. Keeble Alberto Carella Georgi I. Nikov Mark Howarth
author_facet	Irsyad N. A. Khairil Anuar Anusuya Banerjee Anthony H. Keeble Alberto Carella Georgi I. Nikov Mark Howarth
author_sort	Irsyad N. A. Khairil Anuar
title	Spy&Go purification of SpyTag-proteins using pseudo-SpyCatcher to access an oligomerization toolbox
title_short	Spy&Go purification of SpyTag-proteins using pseudo-SpyCatcher to access an oligomerization toolbox
title_full	Spy&Go purification of SpyTag-proteins using pseudo-SpyCatcher to access an oligomerization toolbox
title_fullStr	Spy&Go purification of SpyTag-proteins using pseudo-SpyCatcher to access an oligomerization toolbox
title_full_unstemmed	Spy&Go purification of SpyTag-proteins using pseudo-SpyCatcher to access an oligomerization toolbox
title_sort	spy&go purification of spytag-proteins using pseudo-spycatcher to access an oligomerization toolbox
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/7888920400c8448ebf0e86fe447eb9a5
work_keys_str_mv	AT irsyadnakhairilanuar spygopurificationofspytagproteinsusingpseudospycatchertoaccessanoligomerizationtoolbox AT anusuyabanerjee spygopurificationofspytagproteinsusingpseudospycatchertoaccessanoligomerizationtoolbox AT anthonyhkeeble spygopurificationofspytagproteinsusingpseudospycatchertoaccessanoligomerizationtoolbox AT albertocarella spygopurificationofspytagproteinsusingpseudospycatchertoaccessanoligomerizationtoolbox AT georgiinikov spygopurificationofspytagproteinsusingpseudospycatchertoaccessanoligomerizationtoolbox AT markhowarth spygopurificationofspytagproteinsusingpseudospycatchertoaccessanoligomerizationtoolbox
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Spy&Go purification of SpyTag-proteins using pseudo-SpyCatcher to access an oligomerization toolbox

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