Spy&Go purification of SpyTag-proteins using pseudo-SpyCatcher to access an oligomerization toolbox
Peptide tags are important for protein purification but have minimal benefits afterwards. Here the authors present Spy&Go, which uses rational engineering of the SpyDock protein to capture SpyTag peptides without requiring an irreverisble isopeptide bond.
Guardado en:
Autores principales: | Irsyad N. A. Khairil Anuar, Anusuya Banerjee, Anthony H. Keeble, Alberto Carella, Georgi I. Nikov, Mark Howarth |
---|---|
Formato: | article |
Lenguaje: | EN |
Publicado: |
Nature Portfolio
2019
|
Materias: | |
Acceso en línea: | https://doaj.org/article/7888920400c8448ebf0e86fe447eb9a5 |
Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
Ejemplares similares
-
One-step construction of circularized nanodiscs using SpyCatcher-SpyTag
por: Shanwen Zhang, et al.
Publicado: (2021) -
Retargeting Lentiviruses via SpyCatcher-SpyTag Chemistry for Gene Delivery into Specific Cell Types
por: Nagarjun Kasaraneni, et al.
Publicado: (2017) -
Development of a yeast cell surface display method using the SpyTag/SpyCatcher system
por: Kaho Kajiwara, et al.
Publicado: (2021) -
A perfect spy
por: Le Carre, John
Publicado: (1987) -
A COVID-19 vaccine candidate using SpyCatcher multimerization of the SARS-CoV-2 spike protein receptor-binding domain induces potent neutralising antibody responses
por: Tiong Kit Tan, et al.
Publicado: (2021)