PpMYB36 Encodes a MYB-Type Transcription Factor That Is Involved in Russet Skin Coloration in Pear (Pyrus pyrifolia)
Fruit color is one of the most important external qualities of pear (Pyrus pyrifolia) fruits. However, the mechanisms that control russet skin coloration in pear have not been well characterized. Here, we explored the molecular mechanisms that determine the russet skin trait in pear using the F1 pop...
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Frontiers Media S.A.
2021
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oai:doaj.org-article:788ee6d4143d4d13ac0c1d36efd827aa2021-11-08T08:07:45ZPpMYB36 Encodes a MYB-Type Transcription Factor That Is Involved in Russet Skin Coloration in Pear (Pyrus pyrifolia)1664-462X10.3389/fpls.2021.776816https://doaj.org/article/788ee6d4143d4d13ac0c1d36efd827aa2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.776816/fullhttps://doaj.org/toc/1664-462XFruit color is one of the most important external qualities of pear (Pyrus pyrifolia) fruits. However, the mechanisms that control russet skin coloration in pear have not been well characterized. Here, we explored the molecular mechanisms that determine the russet skin trait in pear using the F1 population derived from a cross between russet skin (‘Niitaka’) and non-russet skin (‘Dangshansu’) cultivars. Pigment measurements indicated that the lignin content in the skin of the russet pear fruits was greater than that in the non-russet pear skin. Genetic analysis revealed that the phenotype of the russet skin pear is associated with an allele of the PpRus gene. Using bulked segregant analysis combined with the genome sequencing (BSA-seq), we identified two simple sequence repeat (SSR) marker loci linked with the russet-colored skin trait in pear. Linkage analysis showed that the PpRus locus maps to the scaffold NW_008988489.1: 53297-211921 on chromosome 8 in the pear genome. In the mapped region, the expression level of LOC103929640 was significantly increased in the russet skin pear and showed a correlation with the increase of lignin content during the ripening period. Genotyping results demonstrated that LOC103929640 encoding the transcription factor MYB36 is the causal gene for the russet skin trait in pear. Particularly, a W-box insertion at the PpMYB36 promoter of russet skin pears is essential for PpMYB36-mediated regulation of lignin accumulation and russet coloration in pear. Overall, these results show that PpMYB36 is involved in the regulation of russet skin trait in pear.Changqing MaChangqing MaXu WangXu WangMengyuan YuMengyuan YuXiaodong ZhengXiaodong ZhengZhijuan SunXiaoli LiuXiaoli LiuYike TianYike TianCaihong WangCaihong WangFrontiers Media S.A.articlePyrus pyrifoliarusset skinlignin biosynthesisSSR markersPpMYB36Plant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021) |
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| collection |
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| language |
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| topic |
Pyrus pyrifolia russet skin lignin biosynthesis SSR markers PpMYB36 Plant culture SB1-1110 |
| spellingShingle |
Pyrus pyrifolia russet skin lignin biosynthesis SSR markers PpMYB36 Plant culture SB1-1110 Changqing Ma Changqing Ma Xu Wang Xu Wang Mengyuan Yu Mengyuan Yu Xiaodong Zheng Xiaodong Zheng Zhijuan Sun Xiaoli Liu Xiaoli Liu Yike Tian Yike Tian Caihong Wang Caihong Wang PpMYB36 Encodes a MYB-Type Transcription Factor That Is Involved in Russet Skin Coloration in Pear (Pyrus pyrifolia) |
| description |
Fruit color is one of the most important external qualities of pear (Pyrus pyrifolia) fruits. However, the mechanisms that control russet skin coloration in pear have not been well characterized. Here, we explored the molecular mechanisms that determine the russet skin trait in pear using the F1 population derived from a cross between russet skin (‘Niitaka’) and non-russet skin (‘Dangshansu’) cultivars. Pigment measurements indicated that the lignin content in the skin of the russet pear fruits was greater than that in the non-russet pear skin. Genetic analysis revealed that the phenotype of the russet skin pear is associated with an allele of the PpRus gene. Using bulked segregant analysis combined with the genome sequencing (BSA-seq), we identified two simple sequence repeat (SSR) marker loci linked with the russet-colored skin trait in pear. Linkage analysis showed that the PpRus locus maps to the scaffold NW_008988489.1: 53297-211921 on chromosome 8 in the pear genome. In the mapped region, the expression level of LOC103929640 was significantly increased in the russet skin pear and showed a correlation with the increase of lignin content during the ripening period. Genotyping results demonstrated that LOC103929640 encoding the transcription factor MYB36 is the causal gene for the russet skin trait in pear. Particularly, a W-box insertion at the PpMYB36 promoter of russet skin pears is essential for PpMYB36-mediated regulation of lignin accumulation and russet coloration in pear. Overall, these results show that PpMYB36 is involved in the regulation of russet skin trait in pear. |
| format |
article |
| author |
Changqing Ma Changqing Ma Xu Wang Xu Wang Mengyuan Yu Mengyuan Yu Xiaodong Zheng Xiaodong Zheng Zhijuan Sun Xiaoli Liu Xiaoli Liu Yike Tian Yike Tian Caihong Wang Caihong Wang |
| author_facet |
Changqing Ma Changqing Ma Xu Wang Xu Wang Mengyuan Yu Mengyuan Yu Xiaodong Zheng Xiaodong Zheng Zhijuan Sun Xiaoli Liu Xiaoli Liu Yike Tian Yike Tian Caihong Wang Caihong Wang |
| author_sort |
Changqing Ma |
| title |
PpMYB36 Encodes a MYB-Type Transcription Factor That Is Involved in Russet Skin Coloration in Pear (Pyrus pyrifolia) |
| title_short |
PpMYB36 Encodes a MYB-Type Transcription Factor That Is Involved in Russet Skin Coloration in Pear (Pyrus pyrifolia) |
| title_full |
PpMYB36 Encodes a MYB-Type Transcription Factor That Is Involved in Russet Skin Coloration in Pear (Pyrus pyrifolia) |
| title_fullStr |
PpMYB36 Encodes a MYB-Type Transcription Factor That Is Involved in Russet Skin Coloration in Pear (Pyrus pyrifolia) |
| title_full_unstemmed |
PpMYB36 Encodes a MYB-Type Transcription Factor That Is Involved in Russet Skin Coloration in Pear (Pyrus pyrifolia) |
| title_sort |
ppmyb36 encodes a myb-type transcription factor that is involved in russet skin coloration in pear (pyrus pyrifolia) |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/788ee6d4143d4d13ac0c1d36efd827aa |
| work_keys_str_mv |
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