THE EFFECT OF GLUT4 EXPRESSION IN HIPPOCAMPAL NEURONS TO SPATIAL MEMORY OF DIABETES-INDUCED RATTUS NOVERGICUS
Background: Identification of appropriate biomarker involved in the pathophysiology of diabetes-associated cognitive decline in animal model is necessary. Glucose transporter 4 (GLUT4) is the component of insulin-dependent neuronal signaling pathway needed for maintaining cognitive function. It is h...
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| Autores principales: | , , , , , , , , |
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| Formato: | article |
| Lenguaje: | EN ID |
| Publicado: |
University of Brawijaya
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/788ee86f26bb46cdbe4500eb56cac762 |
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| Sumario: | Background: Identification of appropriate biomarker involved in the pathophysiology of diabetes-associated cognitive decline in animal model is necessary. Glucose transporter 4 (GLUT4) is the component of insulin-dependent neuronal signaling pathway needed for maintaining cognitive function. It is highly expressed in hippocampal neurons.
Objective: To investigate the effect of GLUT4 expression in hippocampal neurons on the spatial memory of diabetes-induced Rattus novergicus.
Methods: This experimental study used 24 male Rattus novergicus divided into control groups terminated on day-0 (C0), 14 (C1) and 28 (C2) and diabetic groups terminated on day-0 (D0), 14 (D1) and 28 (D2). Diabetes was induced using streptozotocin injections intraperitoneally. Spatial memory (travel time) of rats was assessed on day-14 and 28. Brain tissue sampling was performed for assessment of GLUT4 expression using immunohistochemical methods. Significant differences in means of GLUT4 expression and travel time between groups on day-0, 14 and 28 as well as correlation between means of GLUT4 expression and travel time in D1 and D2 were analyzed statistically.
Results: The mean of GLUT4 expression in diabetic group was significantly higher compared to control on day-14, but not on day-0 and 28. The immunohistochemical examination confirmed this results. There were no significant differences in mean of travel time between control and diabetic groups on day-14 and 28. There were no significant correlation between means of GLUT4 expression and travel time in diabetic groups on day-14 and 28.
Conclusion: The GLUT4 expression of hippocampal neurons did not significantly affect spatial memory of diabetes-induced Rattus novergicus. |
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