Effects of Combined Nitrogen Deficient and Mixotrophic (+Glucose) Culture on the Lipid Accumulation of <i>Parachlorella Kessleri</i> TY

The combustion explosion of ordinary diesel is a global environmental problem. Green microalgae, which do not cause eutrophication, are a raw material that can be used to clean biodiesel. To increase lipid productivity, this study used a nitrogen deficient & mixotrophic (+Glucose) culture of lip...

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Autores principales:	Yifan Gao, Li Ji, Jia Feng, Junping Lv, Shulian Xie
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Parachlorella kessleri</i> TY nitrogen deficient & mixotrophic (+Glucose) culture lipid Hydraulic engineering TC1-978 Water supply for domestic and industrial purposes TD201-500
Acceso en línea:	https://doaj.org/article/7892aa4d383144628c666102d5d9a2dd
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spelling	oai:doaj.org-article:7892aa4d383144628c666102d5d9a2dd2021-11-11T19:56:18ZEffects of Combined Nitrogen Deficient and Mixotrophic (+Glucose) Culture on the Lipid Accumulation of <i>Parachlorella Kessleri</i> TY10.3390/w132130662073-4441https://doaj.org/article/7892aa4d383144628c666102d5d9a2dd2021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4441/13/21/3066https://doaj.org/toc/2073-4441The combustion explosion of ordinary diesel is a global environmental problem. Green microalgae, which do not cause eutrophication, are a raw material that can be used to clean biodiesel. To increase lipid productivity, this study used a nitrogen deficient & mixotrophic (+Glucose) culture of lipid-producing microalgae <i>Parachlorella kessleri</i> TY from Shanxi Province, China. To examine the growth of <i>P. kessleri</i> TY, we measured dry weight, chlorophyll content, and chlorophyll fluorescence intensity under different culture conditions, in addition to the contents of neutral lipids, total lipids, and fatty acids, to examine its lipid accumulation ability. Cells were cultured in autotrophic, nitrogen deficient, mixotrophic (+Glucose), and nitrogen deficient & mixotrophic (+Glucose) conditions for 7 days. We found the growth of <i>P. kessleri</i> TY under nitrogen deficient & mixotrophic conditions was higher than that under the autotrophic and nitrogen deficient conditions, but lower than that under the mixotrophic (+Glucose) conditions. However, its lipid accumulation ability was significantly higher than that of control cultures. In conclusion, <i>P. kessleri</i> TY cultured under nitrogen-deficient and mixotrophic (+Glucose) conditions has significant lipid production capacity. Our results provide a theoretical basis for the use of microalgae as a raw material in the production of biodiesel, and promote the application of <i>P. kessleri</i> TY in large-scale production.Yifan GaoLi JiJia FengJunping LvShulian XieMDPI AGarticle<i>Parachlorella kessleri</i> TYnitrogen deficient & mixotrophic (+Glucose) culturelipidHydraulic engineeringTC1-978Water supply for domestic and industrial purposesTD201-500ENWater, Vol 13, Iss 3066, p 3066 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Parachlorella kessleri</i> TY nitrogen deficient & mixotrophic (+Glucose) culture lipid Hydraulic engineering TC1-978 Water supply for domestic and industrial purposes TD201-500
spellingShingle	<i>Parachlorella kessleri</i> TY nitrogen deficient & mixotrophic (+Glucose) culture lipid Hydraulic engineering TC1-978 Water supply for domestic and industrial purposes TD201-500 Yifan Gao Li Ji Jia Feng Junping Lv Shulian Xie Effects of Combined Nitrogen Deficient and Mixotrophic (+Glucose) Culture on the Lipid Accumulation of <i>Parachlorella Kessleri</i> TY
description	The combustion explosion of ordinary diesel is a global environmental problem. Green microalgae, which do not cause eutrophication, are a raw material that can be used to clean biodiesel. To increase lipid productivity, this study used a nitrogen deficient & mixotrophic (+Glucose) culture of lipid-producing microalgae <i>Parachlorella kessleri</i> TY from Shanxi Province, China. To examine the growth of <i>P. kessleri</i> TY, we measured dry weight, chlorophyll content, and chlorophyll fluorescence intensity under different culture conditions, in addition to the contents of neutral lipids, total lipids, and fatty acids, to examine its lipid accumulation ability. Cells were cultured in autotrophic, nitrogen deficient, mixotrophic (+Glucose), and nitrogen deficient & mixotrophic (+Glucose) conditions for 7 days. We found the growth of <i>P. kessleri</i> TY under nitrogen deficient & mixotrophic conditions was higher than that under the autotrophic and nitrogen deficient conditions, but lower than that under the mixotrophic (+Glucose) conditions. However, its lipid accumulation ability was significantly higher than that of control cultures. In conclusion, <i>P. kessleri</i> TY cultured under nitrogen-deficient and mixotrophic (+Glucose) conditions has significant lipid production capacity. Our results provide a theoretical basis for the use of microalgae as a raw material in the production of biodiesel, and promote the application of <i>P. kessleri</i> TY in large-scale production.
format	article
author	Yifan Gao Li Ji Jia Feng Junping Lv Shulian Xie
author_facet	Yifan Gao Li Ji Jia Feng Junping Lv Shulian Xie
author_sort	Yifan Gao
title	Effects of Combined Nitrogen Deficient and Mixotrophic (+Glucose) Culture on the Lipid Accumulation of <i>Parachlorella Kessleri</i> TY
title_short	Effects of Combined Nitrogen Deficient and Mixotrophic (+Glucose) Culture on the Lipid Accumulation of <i>Parachlorella Kessleri</i> TY
title_full	Effects of Combined Nitrogen Deficient and Mixotrophic (+Glucose) Culture on the Lipid Accumulation of <i>Parachlorella Kessleri</i> TY
title_fullStr	Effects of Combined Nitrogen Deficient and Mixotrophic (+Glucose) Culture on the Lipid Accumulation of <i>Parachlorella Kessleri</i> TY
title_full_unstemmed	Effects of Combined Nitrogen Deficient and Mixotrophic (+Glucose) Culture on the Lipid Accumulation of <i>Parachlorella Kessleri</i> TY
title_sort	effects of combined nitrogen deficient and mixotrophic (+glucose) culture on the lipid accumulation of <i>parachlorella kessleri</i> ty
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/7892aa4d383144628c666102d5d9a2dd
work_keys_str_mv	AT yifangao effectsofcombinednitrogendeficientandmixotrophicglucosecultureonthelipidaccumulationofiparachlorellakessleriity AT liji effectsofcombinednitrogendeficientandmixotrophicglucosecultureonthelipidaccumulationofiparachlorellakessleriity AT jiafeng effectsofcombinednitrogendeficientandmixotrophicglucosecultureonthelipidaccumulationofiparachlorellakessleriity AT junpinglv effectsofcombinednitrogendeficientandmixotrophicglucosecultureonthelipidaccumulationofiparachlorellakessleriity AT shulianxie effectsofcombinednitrogendeficientandmixotrophicglucosecultureonthelipidaccumulationofiparachlorellakessleriity
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Effects of Combined Nitrogen Deficient and Mixotrophic (+Glucose) Culture on the Lipid Accumulation of <i>Parachlorella Kessleri</i> TY

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