SELECTION OF PHYLOGENETICALLY CLOSELY-RELATED YERSINIA PESTIS STRAINS DIFFERING IN THEIR VIRULENCE FOR GUINEA PIGS

Genomic, transcriptome or (and) proteomic comparison of closely related virulent and avirulent microbial strains underlies the search for new pathogenicity factors, potential molecular targets for etiotropic therapy, vaccine prevention and immunotherapy of infectious diseases. This investigation was...

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Autores principales:	N. V. Anisimov, T. I. Kombarova, M. E. Platonov, S. A. Ivanov, M. A. Sukhova, S. V. Dentovskaya, A. P. Anisimov
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2016
Materias:	yersinia pestis vole strains selective virulence guinea pigs animalization testicular passage Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/789512d0cc344f5f8e8e363f65ec454a
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spelling	oai:doaj.org-article:789512d0cc344f5f8e8e363f65ec454a2021-11-22T07:09:47ZSELECTION OF PHYLOGENETICALLY CLOSELY-RELATED YERSINIA PESTIS STRAINS DIFFERING IN THEIR VIRULENCE FOR GUINEA PIGS2220-76192313-739810.15789/2220-7619-2015-4-373-376https://doaj.org/article/789512d0cc344f5f8e8e363f65ec454a2016-02-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/354https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398Genomic, transcriptome or (and) proteomic comparison of closely related virulent and avirulent microbial strains underlies the search for new pathogenicity factors, potential molecular targets for etiotropic therapy, vaccine prevention and immunotherapy of infectious diseases. This investigation was aimed in testing the ability of method of testicular animalization to select phylogenetically close pairs of Y. pestis strains, which dramatically differ in their pathogenicity for guinea pigs, from the populations of as a rule subcutaneously avirulent for guinea pigs “vole” strains of the plague pathogen. Animalization of Y. pestis cultures were performed on guinea pig males by fourfold testicular passage with reducing infective dose. There was no correlation between the ability to cause generalized infectious process (death) after testicular and subcutaneous infection of guinea pigs, but testicular passages made it possible to enrich bacterial culture with a portion of microbes displaying high virulence after subcutaneous infection of this animal species. The methodical approach under study can be successfully applied for selection of pairs of phylogenetically closely related bacterial strains, dramatically differing in their degrees of selective virulence.N. V. AnisimovT. I. KombarovaM. E. PlatonovS. A. IvanovM. A. SukhovaS. V. DentovskayaA. P. AnisimovSankt-Peterburg : NIIÈM imeni Pasteraarticleyersinia pestisvole strainsselective virulenceguinea pigsanimalizationtesticular passageInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 5, Iss 4, Pp 373-376 (2016)
institution	DOAJ
collection	DOAJ
language	RU
topic	yersinia pestis vole strains selective virulence guinea pigs animalization testicular passage Infectious and parasitic diseases RC109-216
spellingShingle	yersinia pestis vole strains selective virulence guinea pigs animalization testicular passage Infectious and parasitic diseases RC109-216 N. V. Anisimov T. I. Kombarova M. E. Platonov S. A. Ivanov M. A. Sukhova S. V. Dentovskaya A. P. Anisimov SELECTION OF PHYLOGENETICALLY CLOSELY-RELATED YERSINIA PESTIS STRAINS DIFFERING IN THEIR VIRULENCE FOR GUINEA PIGS
description	Genomic, transcriptome or (and) proteomic comparison of closely related virulent and avirulent microbial strains underlies the search for new pathogenicity factors, potential molecular targets for etiotropic therapy, vaccine prevention and immunotherapy of infectious diseases. This investigation was aimed in testing the ability of method of testicular animalization to select phylogenetically close pairs of Y. pestis strains, which dramatically differ in their pathogenicity for guinea pigs, from the populations of as a rule subcutaneously avirulent for guinea pigs “vole” strains of the plague pathogen. Animalization of Y. pestis cultures were performed on guinea pig males by fourfold testicular passage with reducing infective dose. There was no correlation between the ability to cause generalized infectious process (death) after testicular and subcutaneous infection of guinea pigs, but testicular passages made it possible to enrich bacterial culture with a portion of microbes displaying high virulence after subcutaneous infection of this animal species. The methodical approach under study can be successfully applied for selection of pairs of phylogenetically closely related bacterial strains, dramatically differing in their degrees of selective virulence.
format	article
author	N. V. Anisimov T. I. Kombarova M. E. Platonov S. A. Ivanov M. A. Sukhova S. V. Dentovskaya A. P. Anisimov
author_facet	N. V. Anisimov T. I. Kombarova M. E. Platonov S. A. Ivanov M. A. Sukhova S. V. Dentovskaya A. P. Anisimov
author_sort	N. V. Anisimov
title	SELECTION OF PHYLOGENETICALLY CLOSELY-RELATED YERSINIA PESTIS STRAINS DIFFERING IN THEIR VIRULENCE FOR GUINEA PIGS
title_short	SELECTION OF PHYLOGENETICALLY CLOSELY-RELATED YERSINIA PESTIS STRAINS DIFFERING IN THEIR VIRULENCE FOR GUINEA PIGS
title_full	SELECTION OF PHYLOGENETICALLY CLOSELY-RELATED YERSINIA PESTIS STRAINS DIFFERING IN THEIR VIRULENCE FOR GUINEA PIGS
title_fullStr	SELECTION OF PHYLOGENETICALLY CLOSELY-RELATED YERSINIA PESTIS STRAINS DIFFERING IN THEIR VIRULENCE FOR GUINEA PIGS
title_full_unstemmed	SELECTION OF PHYLOGENETICALLY CLOSELY-RELATED YERSINIA PESTIS STRAINS DIFFERING IN THEIR VIRULENCE FOR GUINEA PIGS
title_sort	selection of phylogenetically closely-related yersinia pestis strains differing in their virulence for guinea pigs
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2016
url	https://doaj.org/article/789512d0cc344f5f8e8e363f65ec454a
work_keys_str_mv	AT nvanisimov selectionofphylogeneticallycloselyrelatedyersiniapestisstrainsdifferingintheirvirulenceforguineapigs AT tikombarova selectionofphylogeneticallycloselyrelatedyersiniapestisstrainsdifferingintheirvirulenceforguineapigs AT meplatonov selectionofphylogeneticallycloselyrelatedyersiniapestisstrainsdifferingintheirvirulenceforguineapigs AT saivanov selectionofphylogeneticallycloselyrelatedyersiniapestisstrainsdifferingintheirvirulenceforguineapigs AT masukhova selectionofphylogeneticallycloselyrelatedyersiniapestisstrainsdifferingintheirvirulenceforguineapigs AT svdentovskaya selectionofphylogeneticallycloselyrelatedyersiniapestisstrainsdifferingintheirvirulenceforguineapigs AT apanisimov selectionofphylogeneticallycloselyrelatedyersiniapestisstrainsdifferingintheirvirulenceforguineapigs
_version_	1718418049666646016

SELECTION OF PHYLOGENETICALLY CLOSELY-RELATED YERSINIA PESTIS STRAINS DIFFERING IN THEIR VIRULENCE FOR GUINEA PIGS

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