Preparation and Evaluation of Starch Hydrogel/Contact Lens Composites as Epigallocatechin Gallate Delivery Systems for Inhibition of Bacterial Adhesion

Microbial infections caused by wearing contact lenses has become a major health problem, so the design and development of antibacterial contact lenses has attracted widespread attention. To safely and effectively inhibit bacterial adhesion of contact lenses, we have facilely prepared epigallocatechi...

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Autores principales:	Lianghui Zhao, Hongwei Wang, Chengcheng Feng, Fangying Song, Xianli Du
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	starch hydrogel composite contact lens epigallocatechin gallate antibacterial activity Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/78a474ab8cc049b59e511b6c25e32a3b
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spelling	oai:doaj.org-article:78a474ab8cc049b59e511b6c25e32a3b2021-11-16T07:47:00ZPreparation and Evaluation of Starch Hydrogel/Contact Lens Composites as Epigallocatechin Gallate Delivery Systems for Inhibition of Bacterial Adhesion2296-418510.3389/fbioe.2021.759303https://doaj.org/article/78a474ab8cc049b59e511b6c25e32a3b2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fbioe.2021.759303/fullhttps://doaj.org/toc/2296-4185Microbial infections caused by wearing contact lenses has become a major health problem, so the design and development of antibacterial contact lenses has attracted widespread attention. To safely and effectively inhibit bacterial adhesion of contact lenses, we have facilely prepared epigallocatechin gallate (EGCG) loaded starch hydrogel/contact lens composites by in-situ free radical polymerization of the mixture containing 2-hydroxylethyl methacrylate, methacrylic acid and ethylene glycol dimethacrylate. The adequate transmittance of the resulting contact lenses was characterized by ultraviolet-visible spectrophotometry, and their satisfactory stability was examined using differential scanning calorimetry and thermogravimetric analysis. Whereafter, cytotoxicity and degradation experiments were performed to investigate the biocompatibility and degradability of the contact lenses. The results showed the nontoxicity and good degradability of the composites. Besides, the capacity of the contact lenses for in vitro release of EGCG was also evaluated, and the results showed that the EGCG in these contact lenses can be sustainably released for at least 14 days. Further bacterial adhesion assay suggested that the EGCG loaded starch hydrogel/contact lenses could significantly reduce the adhesion of Pseudomonas aeruginosa compared to the control. The EGCG loaded starch hydrogel/contact lens composites provide a potential intervention strategy for preventing ocular microbial infections and inhibiting bacterial keratitis.Lianghui ZhaoLianghui ZhaoLianghui ZhaoHongwei WangHongwei WangChengcheng FengChengcheng FengFangying SongFangying SongXianli DuXianli DuFrontiers Media S.A.articlestarch hydrogelcompositecontact lensepigallocatechin gallateantibacterial activityBiotechnologyTP248.13-248.65ENFrontiers in Bioengineering and Biotechnology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	starch hydrogel composite contact lens epigallocatechin gallate antibacterial activity Biotechnology TP248.13-248.65
spellingShingle	starch hydrogel composite contact lens epigallocatechin gallate antibacterial activity Biotechnology TP248.13-248.65 Lianghui Zhao Lianghui Zhao Lianghui Zhao Hongwei Wang Hongwei Wang Chengcheng Feng Chengcheng Feng Fangying Song Fangying Song Xianli Du Xianli Du Preparation and Evaluation of Starch Hydrogel/Contact Lens Composites as Epigallocatechin Gallate Delivery Systems for Inhibition of Bacterial Adhesion
description	Microbial infections caused by wearing contact lenses has become a major health problem, so the design and development of antibacterial contact lenses has attracted widespread attention. To safely and effectively inhibit bacterial adhesion of contact lenses, we have facilely prepared epigallocatechin gallate (EGCG) loaded starch hydrogel/contact lens composites by in-situ free radical polymerization of the mixture containing 2-hydroxylethyl methacrylate, methacrylic acid and ethylene glycol dimethacrylate. The adequate transmittance of the resulting contact lenses was characterized by ultraviolet-visible spectrophotometry, and their satisfactory stability was examined using differential scanning calorimetry and thermogravimetric analysis. Whereafter, cytotoxicity and degradation experiments were performed to investigate the biocompatibility and degradability of the contact lenses. The results showed the nontoxicity and good degradability of the composites. Besides, the capacity of the contact lenses for in vitro release of EGCG was also evaluated, and the results showed that the EGCG in these contact lenses can be sustainably released for at least 14 days. Further bacterial adhesion assay suggested that the EGCG loaded starch hydrogel/contact lenses could significantly reduce the adhesion of Pseudomonas aeruginosa compared to the control. The EGCG loaded starch hydrogel/contact lens composites provide a potential intervention strategy for preventing ocular microbial infections and inhibiting bacterial keratitis.
format	article
author	Lianghui Zhao Lianghui Zhao Lianghui Zhao Hongwei Wang Hongwei Wang Chengcheng Feng Chengcheng Feng Fangying Song Fangying Song Xianli Du Xianli Du
author_facet	Lianghui Zhao Lianghui Zhao Lianghui Zhao Hongwei Wang Hongwei Wang Chengcheng Feng Chengcheng Feng Fangying Song Fangying Song Xianli Du Xianli Du
author_sort	Lianghui Zhao
title	Preparation and Evaluation of Starch Hydrogel/Contact Lens Composites as Epigallocatechin Gallate Delivery Systems for Inhibition of Bacterial Adhesion
title_short	Preparation and Evaluation of Starch Hydrogel/Contact Lens Composites as Epigallocatechin Gallate Delivery Systems for Inhibition of Bacterial Adhesion
title_full	Preparation and Evaluation of Starch Hydrogel/Contact Lens Composites as Epigallocatechin Gallate Delivery Systems for Inhibition of Bacterial Adhesion
title_fullStr	Preparation and Evaluation of Starch Hydrogel/Contact Lens Composites as Epigallocatechin Gallate Delivery Systems for Inhibition of Bacterial Adhesion
title_full_unstemmed	Preparation and Evaluation of Starch Hydrogel/Contact Lens Composites as Epigallocatechin Gallate Delivery Systems for Inhibition of Bacterial Adhesion
title_sort	preparation and evaluation of starch hydrogel/contact lens composites as epigallocatechin gallate delivery systems for inhibition of bacterial adhesion
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/78a474ab8cc049b59e511b6c25e32a3b
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Preparation and Evaluation of Starch Hydrogel/Contact Lens Composites as Epigallocatechin Gallate Delivery Systems for Inhibition of Bacterial Adhesion

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