<i>Tricholoma matsutake</i>-Derived Peptides Ameliorate Inflammation and Mitochondrial Dysfunction in RAW264.7 Macrophages by Modulating the NF-κB/COX-2 Pathway
<i>Tricholoma matsutake</i> is an edible fungus that contains various bioactive substances, some of them with immunostimulatory properties. Presently, there is limited knowledge about the functional components of <i>T. matsutake</i>. Our aim was to evaluate the protective eff...
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| Main Authors: | , , , |
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| Format: | article |
| Language: | EN |
| Published: |
MDPI AG
2021
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| Subjects: | |
| Online Access: | https://doaj.org/article/78a8c860a1364876be7b9dba0dac7843 |
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| Summary: | <i>Tricholoma matsutake</i> is an edible fungus that contains various bioactive substances, some of them with immunostimulatory properties. Presently, there is limited knowledge about the functional components of <i>T. matsutake</i>. Our aim was to evaluate the protective effects and molecular mechanisms of two <i>T. matsutake</i>-derived peptides, SDLKHFPF and SDIKHFPF, on lipopolysaccharide (LPS)-induced mitochondrial dysfunction and inflammation in RAW264.7 macrophages. <i>Tricholoma matsutake</i> peptides significantly ameliorated the production of inflammatory cytokines and inhibited the expression of COX-2, iNOS, IKKβ, p-IκB-α, and p-NF-κB. Immunofluorescence assays confirmed the inhibitory effect of <i>T. matsutake</i> peptides on NF-κB/p65 nuclear translocation. Furthermore, the treatment with <i>T. matsutake</i> peptides prevented the accumulation of reactive oxygen species, increased the Bcl-2/Bax ratio, reversed the loss of mitochondrial membrane potential, and rescued abnormalities in cellular energy metabolism. These findings indicate that <i>T. matsutake</i> peptides can effectively inhibit the activation of NF-κB/COX-2 and may confer an overall protective effect against LPS-induced cell damage. |
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