Tricholoma matsutake-Derived Peptides Ameliorate Inflammation and Mitochondrial Dysfunction in RAW264.7 Macrophages by Modulating the NF-κB/COX-2 Pathway

Tricholoma matsutake is an edible fungus that contains various bioactive substances, some of them with immunostimulatory properties. Presently, there is limited knowledge about the functional components of T. matsutake. Our aim was to evaluate the protective eff...

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Autores principales:	Mengqi Li, Qi Ge, Hanting Du, Songyi Lin
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Tricholoma matsutake</i> RAW264.7 macrophages inflammation NF-κB mitochondrial dysfunction Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/78a8c860a1364876be7b9dba0dac7843
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spelling	oai:doaj.org-article:78a8c860a1364876be7b9dba0dac78432021-11-25T17:34:09Z<i>Tricholoma matsutake</i>-Derived Peptides Ameliorate Inflammation and Mitochondrial Dysfunction in RAW264.7 Macrophages by Modulating the NF-κB/COX-2 Pathway10.3390/foods101126802304-8158https://doaj.org/article/78a8c860a1364876be7b9dba0dac78432021-11-01T00:00:00Zhttps://www.mdpi.com/2304-8158/10/11/2680https://doaj.org/toc/2304-8158<i>Tricholoma matsutake</i> is an edible fungus that contains various bioactive substances, some of them with immunostimulatory properties. Presently, there is limited knowledge about the functional components of <i>T. matsutake</i>. Our aim was to evaluate the protective effects and molecular mechanisms of two <i>T. matsutake</i>-derived peptides, SDLKHFPF and SDIKHFPF, on lipopolysaccharide (LPS)-induced mitochondrial dysfunction and inflammation in RAW264.7 macrophages. <i>Tricholoma matsutake</i> peptides significantly ameliorated the production of inflammatory cytokines and inhibited the expression of COX-2, iNOS, IKKβ, p-IκB-α, and p-NF-κB. Immunofluorescence assays confirmed the inhibitory effect of <i>T. matsutake</i> peptides on NF-κB/p65 nuclear translocation. Furthermore, the treatment with <i>T. matsutake</i> peptides prevented the accumulation of reactive oxygen species, increased the Bcl-2/Bax ratio, reversed the loss of mitochondrial membrane potential, and rescued abnormalities in cellular energy metabolism. These findings indicate that <i>T. matsutake</i> peptides can effectively inhibit the activation of NF-κB/COX-2 and may confer an overall protective effect against LPS-induced cell damage.Mengqi LiQi GeHanting DuSongyi LinMDPI AGarticle<i>Tricholoma matsutake</i>RAW264.7 macrophagesinflammationNF-κBmitochondrial dysfunctionChemical technologyTP1-1185ENFoods, Vol 10, Iss 2680, p 2680 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Tricholoma matsutake</i> RAW264.7 macrophages inflammation NF-κB mitochondrial dysfunction Chemical technology TP1-1185
spellingShingle	<i>Tricholoma matsutake</i> RAW264.7 macrophages inflammation NF-κB mitochondrial dysfunction Chemical technology TP1-1185 Mengqi Li Qi Ge Hanting Du Songyi Lin <i>Tricholoma matsutake</i>-Derived Peptides Ameliorate Inflammation and Mitochondrial Dysfunction in RAW264.7 Macrophages by Modulating the NF-κB/COX-2 Pathway
description	<i>Tricholoma matsutake</i> is an edible fungus that contains various bioactive substances, some of them with immunostimulatory properties. Presently, there is limited knowledge about the functional components of <i>T. matsutake</i>. Our aim was to evaluate the protective effects and molecular mechanisms of two <i>T. matsutake</i>-derived peptides, SDLKHFPF and SDIKHFPF, on lipopolysaccharide (LPS)-induced mitochondrial dysfunction and inflammation in RAW264.7 macrophages. <i>Tricholoma matsutake</i> peptides significantly ameliorated the production of inflammatory cytokines and inhibited the expression of COX-2, iNOS, IKKβ, p-IκB-α, and p-NF-κB. Immunofluorescence assays confirmed the inhibitory effect of <i>T. matsutake</i> peptides on NF-κB/p65 nuclear translocation. Furthermore, the treatment with <i>T. matsutake</i> peptides prevented the accumulation of reactive oxygen species, increased the Bcl-2/Bax ratio, reversed the loss of mitochondrial membrane potential, and rescued abnormalities in cellular energy metabolism. These findings indicate that <i>T. matsutake</i> peptides can effectively inhibit the activation of NF-κB/COX-2 and may confer an overall protective effect against LPS-induced cell damage.
format	article
author	Mengqi Li Qi Ge Hanting Du Songyi Lin
author_facet	Mengqi Li Qi Ge Hanting Du Songyi Lin
author_sort	Mengqi Li
title	<i>Tricholoma matsutake</i>-Derived Peptides Ameliorate Inflammation and Mitochondrial Dysfunction in RAW264.7 Macrophages by Modulating the NF-κB/COX-2 Pathway
title_short	<i>Tricholoma matsutake</i>-Derived Peptides Ameliorate Inflammation and Mitochondrial Dysfunction in RAW264.7 Macrophages by Modulating the NF-κB/COX-2 Pathway
title_full	<i>Tricholoma matsutake</i>-Derived Peptides Ameliorate Inflammation and Mitochondrial Dysfunction in RAW264.7 Macrophages by Modulating the NF-κB/COX-2 Pathway
title_fullStr	<i>Tricholoma matsutake</i>-Derived Peptides Ameliorate Inflammation and Mitochondrial Dysfunction in RAW264.7 Macrophages by Modulating the NF-κB/COX-2 Pathway
title_full_unstemmed	<i>Tricholoma matsutake</i>-Derived Peptides Ameliorate Inflammation and Mitochondrial Dysfunction in RAW264.7 Macrophages by Modulating the NF-κB/COX-2 Pathway
title_sort	<i>tricholoma matsutake</i>-derived peptides ameliorate inflammation and mitochondrial dysfunction in raw264.7 macrophages by modulating the nf-κb/cox-2 pathway
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/78a8c860a1364876be7b9dba0dac7843
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<i>Tricholoma matsutake</i>-Derived Peptides Ameliorate Inflammation and Mitochondrial Dysfunction in RAW264.7 Macrophages by Modulating the NF-κB/COX-2 Pathway

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