Genomic evidence of environmental and resident Salmonella Senftenberg and Montevideo contamination in the pistachio supply-chain.

Pistachios have been implicated in two salmonellosis outbreaks and multiple recalls in the U.S. This study performed an in-depth retrospective data analysis of Salmonella associated with pistachios as well as a storage study to evaluate the survivability of Salmonella on inoculated inshell pistachio...

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Autores principales:	Julie Haendiges, Gordon R Davidson, James B Pettengill, Elizabeth Reed, Padmini Ramachandran, Tyann Blessington, Jesse D Miller, Nathan Anderson, Sam Myoda, Eric W Brown, Jie Zheng, Rohan Tikekar, Maria Hoffmann
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/78b2300d3d7642c9aed29ace54ff973c
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spelling	oai:doaj.org-article:78b2300d3d7642c9aed29ace54ff973c2021-12-02T20:04:20ZGenomic evidence of environmental and resident Salmonella Senftenberg and Montevideo contamination in the pistachio supply-chain.1932-620310.1371/journal.pone.0259471https://doaj.org/article/78b2300d3d7642c9aed29ace54ff973c2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0259471https://doaj.org/toc/1932-6203Pistachios have been implicated in two salmonellosis outbreaks and multiple recalls in the U.S. This study performed an in-depth retrospective data analysis of Salmonella associated with pistachios as well as a storage study to evaluate the survivability of Salmonella on inoculated inshell pistachios to further understand the genetics and microbiological dynamics of this commodity-pathogen pair. The retrospective data analysis on isolates associated with pistachios was performed utilizing short-read and long-read sequencing technologies. The sequence data were analyzed using two methods: the FDA's Center for Food Safety and Applied Nutrition Single Nucleotide Polymorphism (SNP) analysis and Whole Genome Multilocus Sequence Typing (wgMLST). The year-long storage study evaluated the survival of five strains of Salmonella on pistachios stored at 25 °C at 35% and 54% relative humidity (RH). Our results demonstrate: i) evidence of persistent Salmonella Senftenberg and Salmonella Montevideo strains in pistachio environments, some of which may be due to clonal resident strains and some of which may be due to preharvest contamination; ii) presence of the Copper Homeostasis and Silver Resistance Island (CHASRI) in Salmonella Senftenberg and Montevideo strains in the pistachio supply chain; and iii) the use of metagenomic analysis is a novel tool for determining the composition of serovar survival in a cocktail inoculated storage study.Julie HaendigesGordon R DavidsonJames B PettengillElizabeth ReedPadmini RamachandranTyann BlessingtonJesse D MillerNathan AndersonSam MyodaEric W BrownJie ZhengRohan TikekarMaria HoffmannPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11, p e0259471 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Julie Haendiges Gordon R Davidson James B Pettengill Elizabeth Reed Padmini Ramachandran Tyann Blessington Jesse D Miller Nathan Anderson Sam Myoda Eric W Brown Jie Zheng Rohan Tikekar Maria Hoffmann Genomic evidence of environmental and resident Salmonella Senftenberg and Montevideo contamination in the pistachio supply-chain.
description	Pistachios have been implicated in two salmonellosis outbreaks and multiple recalls in the U.S. This study performed an in-depth retrospective data analysis of Salmonella associated with pistachios as well as a storage study to evaluate the survivability of Salmonella on inoculated inshell pistachios to further understand the genetics and microbiological dynamics of this commodity-pathogen pair. The retrospective data analysis on isolates associated with pistachios was performed utilizing short-read and long-read sequencing technologies. The sequence data were analyzed using two methods: the FDA's Center for Food Safety and Applied Nutrition Single Nucleotide Polymorphism (SNP) analysis and Whole Genome Multilocus Sequence Typing (wgMLST). The year-long storage study evaluated the survival of five strains of Salmonella on pistachios stored at 25 °C at 35% and 54% relative humidity (RH). Our results demonstrate: i) evidence of persistent Salmonella Senftenberg and Salmonella Montevideo strains in pistachio environments, some of which may be due to clonal resident strains and some of which may be due to preharvest contamination; ii) presence of the Copper Homeostasis and Silver Resistance Island (CHASRI) in Salmonella Senftenberg and Montevideo strains in the pistachio supply chain; and iii) the use of metagenomic analysis is a novel tool for determining the composition of serovar survival in a cocktail inoculated storage study.
format	article
author	Julie Haendiges Gordon R Davidson James B Pettengill Elizabeth Reed Padmini Ramachandran Tyann Blessington Jesse D Miller Nathan Anderson Sam Myoda Eric W Brown Jie Zheng Rohan Tikekar Maria Hoffmann
author_facet	Julie Haendiges Gordon R Davidson James B Pettengill Elizabeth Reed Padmini Ramachandran Tyann Blessington Jesse D Miller Nathan Anderson Sam Myoda Eric W Brown Jie Zheng Rohan Tikekar Maria Hoffmann
author_sort	Julie Haendiges
title	Genomic evidence of environmental and resident Salmonella Senftenberg and Montevideo contamination in the pistachio supply-chain.
title_short	Genomic evidence of environmental and resident Salmonella Senftenberg and Montevideo contamination in the pistachio supply-chain.
title_full	Genomic evidence of environmental and resident Salmonella Senftenberg and Montevideo contamination in the pistachio supply-chain.
title_fullStr	Genomic evidence of environmental and resident Salmonella Senftenberg and Montevideo contamination in the pistachio supply-chain.
title_full_unstemmed	Genomic evidence of environmental and resident Salmonella Senftenberg and Montevideo contamination in the pistachio supply-chain.
title_sort	genomic evidence of environmental and resident salmonella senftenberg and montevideo contamination in the pistachio supply-chain.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/78b2300d3d7642c9aed29ace54ff973c
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Genomic evidence of environmental and resident Salmonella Senftenberg and Montevideo contamination in the pistachio supply-chain.

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