Antileishmanial activity and cytotoxicity of ZnO-based nano-formulations

Samina Nazir,1 Atiya Rabbani,2 Khalid Mehmood,3 Farhana Maqbool,4 Ghulam Mujtaba Shah,4 Muhammad Fiaz Khan,5 Muhammad Sajid2 1Department of Chemistry, College of Science, King Faisal University, Al-Ahsa, Saudi Arabia; 2Department of Biochemistry, Hazara University, Mansehra, Pakistan; 3Medical Centr...

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Autores principales:	Nazir S, Rabbani A, Mehmood K, Maqbool F, Shah GM, Khan MF, Sajid M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	ZnO nanoparticles Doped-ZnO NP toxicity In-vivo toxicity Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/78b5b6f4623e41dc855e18411f5d7ceb
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spelling	oai:doaj.org-article:78b5b6f4623e41dc855e18411f5d7ceb2021-12-02T11:05:12ZAntileishmanial activity and cytotoxicity of ZnO-based nano-formulations1178-2013https://doaj.org/article/78b5b6f4623e41dc855e18411f5d7ceb2019-09-01T00:00:00Zhttps://www.dovepress.com/antileishmanial-activity-and-cytotoxicity-of-zno-based-nano-formulatio-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Samina Nazir,1 Atiya Rabbani,2 Khalid Mehmood,3 Farhana Maqbool,4 Ghulam Mujtaba Shah,4 Muhammad Fiaz Khan,5 Muhammad Sajid2 1Department of Chemistry, College of Science, King Faisal University, Al-Ahsa, Saudi Arabia; 2Department of Biochemistry, Hazara University, Mansehra, Pakistan; 3Medical Centre, Quaid-e-Azam University, Islamabad, Pakistan; 4Department of Microbiology, Hazara University, Mansehra, Pakistan; 5Department of Zoology, Hazara University, Mansehra, PakistanCorrespondence: Muhammad SajidDepartment of Biochemistry, Hazara University, Mansehra, PakistanTel +92 333 507 1757Fax +92 99 741 4111Email sajid931@hotmail.comIntroduction: Nanoparticles (NPs) can be toxic due to their nano-range sizes. Zinc oxide (ZnO) has good biocompatibility and is commercially used in cosmetics. Moreover, ZnO NPs have potential biomedical uses, but their safety remains unclear.Methods: A range of doped ZnO NPs was evaluated for antileishmanial activity and in vitro toxicity in brine shrimp and human macrophages, and N-doped ZnO NPs were evaluated for in vivo toxicity in male BALB/C mice. N-doped ZnO NPs were administered via two routes: intra-peritoneal injection and topically as a paste. The dosages were 10, 50, and 100 mg/kg/day for 14 days.Results: Topical administration was safe at all dosages, but intra-peritoneal injection displayed toxicity at higher doses, namely, 50 and 100 mg/kg/day. The pathological results for the i.p. dose groups were mild to severe degenerative changes in parenchyma cells, increases in Kupffer cells, disappearance of hepatic plates, increases in cell size, ballooning, cytoplasmic changes, and nuclear pyknosis in the liver. Kidney histology was also altered in the i.p. administration group (dose 100 mg/kg/day), with inflammatory changes in the focal area. We associate pathological abnormalities with the presence of doped ZnO NPs at the diseased site, which was verified by PIXE analysis of the liver and kidney samples of the treated and untreated mice groups.Conclusion: The toxicity of the doped ZnO NPs can serve as an essential determinant for the effects of ZnO NPs on environmental toxicity and can be used for guidelines for safer use of ZnO-based nanomaterials in topical treatment of leishmaniasis and other biomedical applications.Keywords: ZnO, nanoparticles, doped-ZnO, NP toxicity, in vivo toxicityNazir SRabbani AMehmood KMaqbool FShah GMKhan MFSajid MDove Medical PressarticleZnOnanoparticlesDoped-ZnONP toxicityIn-vivo toxicityMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 7809-7822 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	ZnO nanoparticles Doped-ZnO NP toxicity In-vivo toxicity Medicine (General) R5-920
spellingShingle	ZnO nanoparticles Doped-ZnO NP toxicity In-vivo toxicity Medicine (General) R5-920 Nazir S Rabbani A Mehmood K Maqbool F Shah GM Khan MF Sajid M Antileishmanial activity and cytotoxicity of ZnO-based nano-formulations
description	Samina Nazir,1 Atiya Rabbani,2 Khalid Mehmood,3 Farhana Maqbool,4 Ghulam Mujtaba Shah,4 Muhammad Fiaz Khan,5 Muhammad Sajid2 1Department of Chemistry, College of Science, King Faisal University, Al-Ahsa, Saudi Arabia; 2Department of Biochemistry, Hazara University, Mansehra, Pakistan; 3Medical Centre, Quaid-e-Azam University, Islamabad, Pakistan; 4Department of Microbiology, Hazara University, Mansehra, Pakistan; 5Department of Zoology, Hazara University, Mansehra, PakistanCorrespondence: Muhammad SajidDepartment of Biochemistry, Hazara University, Mansehra, PakistanTel +92 333 507 1757Fax +92 99 741 4111Email sajid931@hotmail.comIntroduction: Nanoparticles (NPs) can be toxic due to their nano-range sizes. Zinc oxide (ZnO) has good biocompatibility and is commercially used in cosmetics. Moreover, ZnO NPs have potential biomedical uses, but their safety remains unclear.Methods: A range of doped ZnO NPs was evaluated for antileishmanial activity and in vitro toxicity in brine shrimp and human macrophages, and N-doped ZnO NPs were evaluated for in vivo toxicity in male BALB/C mice. N-doped ZnO NPs were administered via two routes: intra-peritoneal injection and topically as a paste. The dosages were 10, 50, and 100 mg/kg/day for 14 days.Results: Topical administration was safe at all dosages, but intra-peritoneal injection displayed toxicity at higher doses, namely, 50 and 100 mg/kg/day. The pathological results for the i.p. dose groups were mild to severe degenerative changes in parenchyma cells, increases in Kupffer cells, disappearance of hepatic plates, increases in cell size, ballooning, cytoplasmic changes, and nuclear pyknosis in the liver. Kidney histology was also altered in the i.p. administration group (dose 100 mg/kg/day), with inflammatory changes in the focal area. We associate pathological abnormalities with the presence of doped ZnO NPs at the diseased site, which was verified by PIXE analysis of the liver and kidney samples of the treated and untreated mice groups.Conclusion: The toxicity of the doped ZnO NPs can serve as an essential determinant for the effects of ZnO NPs on environmental toxicity and can be used for guidelines for safer use of ZnO-based nanomaterials in topical treatment of leishmaniasis and other biomedical applications.Keywords: ZnO, nanoparticles, doped-ZnO, NP toxicity, in vivo toxicity
format	article
author	Nazir S Rabbani A Mehmood K Maqbool F Shah GM Khan MF Sajid M
author_facet	Nazir S Rabbani A Mehmood K Maqbool F Shah GM Khan MF Sajid M
author_sort	Nazir S
title	Antileishmanial activity and cytotoxicity of ZnO-based nano-formulations
title_short	Antileishmanial activity and cytotoxicity of ZnO-based nano-formulations
title_full	Antileishmanial activity and cytotoxicity of ZnO-based nano-formulations
title_fullStr	Antileishmanial activity and cytotoxicity of ZnO-based nano-formulations
title_full_unstemmed	Antileishmanial activity and cytotoxicity of ZnO-based nano-formulations
title_sort	antileishmanial activity and cytotoxicity of zno-based nano-formulations
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/78b5b6f4623e41dc855e18411f5d7ceb
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Antileishmanial activity and cytotoxicity of ZnO-based nano-formulations

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