Pancreatic human islets and insulin-producing cells derived from embryonic stem cells are rapidly identified by a newly developed Dithizone
Abstract We developed an optimized Dipheylthiocarbazone or Dithizone (DTZ) with improved physical and chemical properties to characterize human islets and insulin-producing cells differentiated from embryonic stem cells. Application of the newly formulated iDTZ (i stands for islet) over a range of t...
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Nature Portfolio
2019
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oai:doaj.org-article:78b909345c634e4cbdad967487951c3c2021-12-02T15:10:03ZPancreatic human islets and insulin-producing cells derived from embryonic stem cells are rapidly identified by a newly developed Dithizone10.1038/s41598-019-45678-y2045-2322https://doaj.org/article/78b909345c634e4cbdad967487951c3c2019-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-45678-yhttps://doaj.org/toc/2045-2322Abstract We developed an optimized Dipheylthiocarbazone or Dithizone (DTZ) with improved physical and chemical properties to characterize human islets and insulin-producing cells differentiated from embryonic stem cells. Application of the newly formulated iDTZ (i stands for islet) over a range of temperatures, time intervals and cell and tissue types found it to be robust for identifying these cells. Through high transition zinc binding, the iDTZ compound concentrated in insulin-producing cells and proved effective at delineating zinc levels in vitro.Bashar KhiatahMeirigeng QiYoujun WuKuan-Tsen ChenRachel PerezLuis ValienteKeiko OmoriJeffrey S. IsenbergFouad KandeelJiing-Kuan YeeIsmail H. Al-AbdullahNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-6 (2019) |
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DOAJ |
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DOAJ |
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Medicine R Science Q |
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Medicine R Science Q Bashar Khiatah Meirigeng Qi Youjun Wu Kuan-Tsen Chen Rachel Perez Luis Valiente Keiko Omori Jeffrey S. Isenberg Fouad Kandeel Jiing-Kuan Yee Ismail H. Al-Abdullah Pancreatic human islets and insulin-producing cells derived from embryonic stem cells are rapidly identified by a newly developed Dithizone |
| description |
Abstract We developed an optimized Dipheylthiocarbazone or Dithizone (DTZ) with improved physical and chemical properties to characterize human islets and insulin-producing cells differentiated from embryonic stem cells. Application of the newly formulated iDTZ (i stands for islet) over a range of temperatures, time intervals and cell and tissue types found it to be robust for identifying these cells. Through high transition zinc binding, the iDTZ compound concentrated in insulin-producing cells and proved effective at delineating zinc levels in vitro. |
| format |
article |
| author |
Bashar Khiatah Meirigeng Qi Youjun Wu Kuan-Tsen Chen Rachel Perez Luis Valiente Keiko Omori Jeffrey S. Isenberg Fouad Kandeel Jiing-Kuan Yee Ismail H. Al-Abdullah |
| author_facet |
Bashar Khiatah Meirigeng Qi Youjun Wu Kuan-Tsen Chen Rachel Perez Luis Valiente Keiko Omori Jeffrey S. Isenberg Fouad Kandeel Jiing-Kuan Yee Ismail H. Al-Abdullah |
| author_sort |
Bashar Khiatah |
| title |
Pancreatic human islets and insulin-producing cells derived from embryonic stem cells are rapidly identified by a newly developed Dithizone |
| title_short |
Pancreatic human islets and insulin-producing cells derived from embryonic stem cells are rapidly identified by a newly developed Dithizone |
| title_full |
Pancreatic human islets and insulin-producing cells derived from embryonic stem cells are rapidly identified by a newly developed Dithizone |
| title_fullStr |
Pancreatic human islets and insulin-producing cells derived from embryonic stem cells are rapidly identified by a newly developed Dithizone |
| title_full_unstemmed |
Pancreatic human islets and insulin-producing cells derived from embryonic stem cells are rapidly identified by a newly developed Dithizone |
| title_sort |
pancreatic human islets and insulin-producing cells derived from embryonic stem cells are rapidly identified by a newly developed dithizone |
| publisher |
Nature Portfolio |
| publishDate |
2019 |
| url |
https://doaj.org/article/78b909345c634e4cbdad967487951c3c |
| work_keys_str_mv |
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