Joint single-cell DNA accessibility and protein epitope profiling reveals environmental regulation of epigenomic heterogeneity

Cellular heterogeneity in cancer is complex and difficult to study. Here, the authors introduce Protein-indexed Assay of Transposase Accessible Chromatin (Pi-ATAC), which combines single cell chromatin and proteomic profiling to provide deep insight into the tumor microenvironment, and reveal the ro...

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Autores principales:	Xingqi Chen, Ulrike M. Litzenburger, Yuning Wei, Alicia N. Schep, Edward L. LaGory, Hani Choudhry, Amato J. Giaccia, William J. Greenleaf, Howard Y. Chang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/78bb993491fb4b9faed443f446fb2949
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spelling	oai:doaj.org-article:78bb993491fb4b9faed443f446fb29492021-12-02T15:34:26ZJoint single-cell DNA accessibility and protein epitope profiling reveals environmental regulation of epigenomic heterogeneity10.1038/s41467-018-07115-y2041-1723https://doaj.org/article/78bb993491fb4b9faed443f446fb29492018-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-07115-yhttps://doaj.org/toc/2041-1723Cellular heterogeneity in cancer is complex and difficult to study. Here, the authors introduce Protein-indexed Assay of Transposase Accessible Chromatin (Pi-ATAC), which combines single cell chromatin and proteomic profiling to provide deep insight into the tumor microenvironment, and reveal the role of hypoxia in shaping the regulome of a subset of breast cancer cells in vivo.Xingqi ChenUlrike M. LitzenburgerYuning WeiAlicia N. SchepEdward L. LaGoryHani ChoudhryAmato J. GiacciaWilliam J. GreenleafHoward Y. ChangNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Xingqi Chen Ulrike M. Litzenburger Yuning Wei Alicia N. Schep Edward L. LaGory Hani Choudhry Amato J. Giaccia William J. Greenleaf Howard Y. Chang Joint single-cell DNA accessibility and protein epitope profiling reveals environmental regulation of epigenomic heterogeneity
description	Cellular heterogeneity in cancer is complex and difficult to study. Here, the authors introduce Protein-indexed Assay of Transposase Accessible Chromatin (Pi-ATAC), which combines single cell chromatin and proteomic profiling to provide deep insight into the tumor microenvironment, and reveal the role of hypoxia in shaping the regulome of a subset of breast cancer cells in vivo.
format	article
author	Xingqi Chen Ulrike M. Litzenburger Yuning Wei Alicia N. Schep Edward L. LaGory Hani Choudhry Amato J. Giaccia William J. Greenleaf Howard Y. Chang
author_facet	Xingqi Chen Ulrike M. Litzenburger Yuning Wei Alicia N. Schep Edward L. LaGory Hani Choudhry Amato J. Giaccia William J. Greenleaf Howard Y. Chang
author_sort	Xingqi Chen
title	Joint single-cell DNA accessibility and protein epitope profiling reveals environmental regulation of epigenomic heterogeneity
title_short	Joint single-cell DNA accessibility and protein epitope profiling reveals environmental regulation of epigenomic heterogeneity
title_full	Joint single-cell DNA accessibility and protein epitope profiling reveals environmental regulation of epigenomic heterogeneity
title_fullStr	Joint single-cell DNA accessibility and protein epitope profiling reveals environmental regulation of epigenomic heterogeneity
title_full_unstemmed	Joint single-cell DNA accessibility and protein epitope profiling reveals environmental regulation of epigenomic heterogeneity
title_sort	joint single-cell dna accessibility and protein epitope profiling reveals environmental regulation of epigenomic heterogeneity
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/78bb993491fb4b9faed443f446fb2949
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Joint single-cell DNA accessibility and protein epitope profiling reveals environmental regulation of epigenomic heterogeneity

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