Development of genic SSR marker resources from RNA-seq data in Camellia japonica and their application in the genus Camellia

Abstract Camellia is a genus of flowering plants in the family Theaceae, and several species in this genus have economic importance. Although a great deal of molecular makers has been developed for molecular assisted breeding in genus Camellia in the past decade, the number of simple sequence repeat...

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Autores principales:	Qingyuan Li, Xiaojun Su, Huanhuan Ma, Kebing Du, Min Yang, Baolin Chen, Shao Fu, Tianjing Fu, Conglin Xiang, Qing Zhao, Lin Xu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/78cb7195840048efad0f7fb6ad5ab3b0
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spelling	oai:doaj.org-article:78cb7195840048efad0f7fb6ad5ab3b02021-12-02T16:57:57ZDevelopment of genic SSR marker resources from RNA-seq data in Camellia japonica and their application in the genus Camellia10.1038/s41598-021-89350-w2045-2322https://doaj.org/article/78cb7195840048efad0f7fb6ad5ab3b02021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89350-whttps://doaj.org/toc/2045-2322Abstract Camellia is a genus of flowering plants in the family Theaceae, and several species in this genus have economic importance. Although a great deal of molecular makers has been developed for molecular assisted breeding in genus Camellia in the past decade, the number of simple sequence repeats (SSRs) publicly available for plants in this genus is insufficient. In this study, a total of 28,854 potential SSRs were identified with a frequency of 4.63 kb. A total of 172 primer pairs were synthesized and preliminarily screened in 10 C. japonica accessions, and of these primer pairs, 111 were found to be polymorphic. Fifty-one polymorphic SSR markers were randomly selected to perform further analysis of the genetic relationships of 89 accessions across the genus Camellia. Cluster analysis revealed major clusters corresponding to those based on taxonomic classification and geographic origin. Furthermore, all the genotypes of C. japonica separated and consistently grouped well in the genetic structure analysis. The results of the present study provide high-quality SSR resources for molecular genetic breeding studies in camellia plants.Qingyuan LiXiaojun SuHuanhuan MaKebing DuMin YangBaolin ChenShao FuTianjing FuConglin XiangQing ZhaoLin XuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Qingyuan Li Xiaojun Su Huanhuan Ma Kebing Du Min Yang Baolin Chen Shao Fu Tianjing Fu Conglin Xiang Qing Zhao Lin Xu Development of genic SSR marker resources from RNA-seq data in Camellia japonica and their application in the genus Camellia
description	Abstract Camellia is a genus of flowering plants in the family Theaceae, and several species in this genus have economic importance. Although a great deal of molecular makers has been developed for molecular assisted breeding in genus Camellia in the past decade, the number of simple sequence repeats (SSRs) publicly available for plants in this genus is insufficient. In this study, a total of 28,854 potential SSRs were identified with a frequency of 4.63 kb. A total of 172 primer pairs were synthesized and preliminarily screened in 10 C. japonica accessions, and of these primer pairs, 111 were found to be polymorphic. Fifty-one polymorphic SSR markers were randomly selected to perform further analysis of the genetic relationships of 89 accessions across the genus Camellia. Cluster analysis revealed major clusters corresponding to those based on taxonomic classification and geographic origin. Furthermore, all the genotypes of C. japonica separated and consistently grouped well in the genetic structure analysis. The results of the present study provide high-quality SSR resources for molecular genetic breeding studies in camellia plants.
format	article
author	Qingyuan Li Xiaojun Su Huanhuan Ma Kebing Du Min Yang Baolin Chen Shao Fu Tianjing Fu Conglin Xiang Qing Zhao Lin Xu
author_facet	Qingyuan Li Xiaojun Su Huanhuan Ma Kebing Du Min Yang Baolin Chen Shao Fu Tianjing Fu Conglin Xiang Qing Zhao Lin Xu
author_sort	Qingyuan Li
title	Development of genic SSR marker resources from RNA-seq data in Camellia japonica and their application in the genus Camellia
title_short	Development of genic SSR marker resources from RNA-seq data in Camellia japonica and their application in the genus Camellia
title_full	Development of genic SSR marker resources from RNA-seq data in Camellia japonica and their application in the genus Camellia
title_fullStr	Development of genic SSR marker resources from RNA-seq data in Camellia japonica and their application in the genus Camellia
title_full_unstemmed	Development of genic SSR marker resources from RNA-seq data in Camellia japonica and their application in the genus Camellia
title_sort	development of genic ssr marker resources from rna-seq data in camellia japonica and their application in the genus camellia
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/78cb7195840048efad0f7fb6ad5ab3b0
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Development of genic SSR marker resources from RNA-seq data in Camellia japonica and their application in the genus Camellia

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