In vivo Toxicity Evaluation of Ethanolic Extract From Two Mangrove Plants

Background: Mangrove plants, often possessing a unique collection of metabolites, have extensively been used for the primary healthcare of coastal residents. Objectives: To determine the safety level and enrich the scientific data, the present study aimed to investigate the toxicity of Avicennia of...

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Autores principales:	Rana Biswas, Md. Abdullah Al Munna, Md. Morsaline Billah, Md. Emdadul Islam, Kazi Mohammed Didarul Islam, Afiya Aunjum
Formato:	article
Lenguaje:	EN
Publicado:	Mazandaran University of Medical Sciences 2021
Materias:	swiss mice artemia toxicity lethal dose (ld50) liver Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/78cd359f29c441c8802bfe935dd9bf32
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spelling	oai:doaj.org-article:78cd359f29c441c8802bfe935dd9bf322021-11-07T16:15:19ZIn vivo Toxicity Evaluation of Ethanolic Extract From Two Mangrove Plants2423-4494https://doaj.org/article/78cd359f29c441c8802bfe935dd9bf322021-02-01T00:00:00Zhttps://pbr.mazums.ac.ir/article-1-391-en.htmlhttps://doaj.org/toc/2423-4494Background: Mangrove plants, often possessing a unique collection of metabolites, have extensively been used for the primary healthcare of coastal residents. Objectives: To determine the safety level and enrich the scientific data, the present study aimed to investigate the toxicity of Avicennia officinalis and Excoecaria agallocha. Methods: Ethanolic leaf and stem extracts were evaluated for cytotoxicity by brine shrimp lethality assay. The obtained extracts were administered to Swiss albino in a single dose (200, 400, 800, 1600, & 3200 mg/kg body weight) by oral-gavage for acute toxicity assay. Furthermore, systematic observation was performed by close monitoring for any toxic manifestations and mortality after dosing for the first 4 h, at 24 h and twice daily for 6 days. Evaluating the adverse effects were estimated by comparing the test groups with the controls. After sacrificing all group animals, relative organ weight was measured and histopathological analysis was conducted. Results: Having Lethal Concentration (LC50) of 44.66 µg/mL, E. agallocha leaf was found with the highest toxicity against brine shrimp nauplii. The toxicological study data demonstrated no death and noticeable change in behavioral patterns in the test mice groups, compared with the control group. Moreover, no significant (P>0.05) differences were found in body weight and relative organ weights, compared to the controls. The histological structures of the liver in the treated mice displayed regular tissue configurations similar to the control group. Conclusion: In this study, the mice model exhibited no harmful effects; thus, the reported results indicated that the ethanolic extracts of leaf and stem of these two mangrove plants are safe for therapeutic use. Further long-term toxicological impact of the extracts should be determined for well-founded confirmation.Rana BiswasMd. Abdullah Al MunnaMd. Morsaline BillahMd. Emdadul IslamKazi Mohammed Didarul Islam Afiya AunjumMazandaran University of Medical Sciencesarticleswiss miceartemiatoxicitylethal dose (ld50)liverTherapeutics. PharmacologyRM1-950ENPharmaceutical and Biomedical Research, Vol 7, Iss 3, Pp 209-216 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	swiss mice artemia toxicity lethal dose (ld50) liver Therapeutics. Pharmacology RM1-950
spellingShingle	swiss mice artemia toxicity lethal dose (ld50) liver Therapeutics. Pharmacology RM1-950 Rana Biswas Md. Abdullah Al Munna Md. Morsaline Billah Md. Emdadul Islam Kazi Mohammed Didarul Islam Afiya Aunjum In vivo Toxicity Evaluation of Ethanolic Extract From Two Mangrove Plants
description	Background: Mangrove plants, often possessing a unique collection of metabolites, have extensively been used for the primary healthcare of coastal residents. Objectives: To determine the safety level and enrich the scientific data, the present study aimed to investigate the toxicity of Avicennia officinalis and Excoecaria agallocha. Methods: Ethanolic leaf and stem extracts were evaluated for cytotoxicity by brine shrimp lethality assay. The obtained extracts were administered to Swiss albino in a single dose (200, 400, 800, 1600, & 3200 mg/kg body weight) by oral-gavage for acute toxicity assay. Furthermore, systematic observation was performed by close monitoring for any toxic manifestations and mortality after dosing for the first 4 h, at 24 h and twice daily for 6 days. Evaluating the adverse effects were estimated by comparing the test groups with the controls. After sacrificing all group animals, relative organ weight was measured and histopathological analysis was conducted. Results: Having Lethal Concentration (LC50) of 44.66 µg/mL, E. agallocha leaf was found with the highest toxicity against brine shrimp nauplii. The toxicological study data demonstrated no death and noticeable change in behavioral patterns in the test mice groups, compared with the control group. Moreover, no significant (P>0.05) differences were found in body weight and relative organ weights, compared to the controls. The histological structures of the liver in the treated mice displayed regular tissue configurations similar to the control group. Conclusion: In this study, the mice model exhibited no harmful effects; thus, the reported results indicated that the ethanolic extracts of leaf and stem of these two mangrove plants are safe for therapeutic use. Further long-term toxicological impact of the extracts should be determined for well-founded confirmation.
format	article
author	Rana Biswas Md. Abdullah Al Munna Md. Morsaline Billah Md. Emdadul Islam Kazi Mohammed Didarul Islam Afiya Aunjum
author_facet	Rana Biswas Md. Abdullah Al Munna Md. Morsaline Billah Md. Emdadul Islam Kazi Mohammed Didarul Islam Afiya Aunjum
author_sort	Rana Biswas
title	In vivo Toxicity Evaluation of Ethanolic Extract From Two Mangrove Plants
title_short	In vivo Toxicity Evaluation of Ethanolic Extract From Two Mangrove Plants
title_full	In vivo Toxicity Evaluation of Ethanolic Extract From Two Mangrove Plants
title_fullStr	In vivo Toxicity Evaluation of Ethanolic Extract From Two Mangrove Plants
title_full_unstemmed	In vivo Toxicity Evaluation of Ethanolic Extract From Two Mangrove Plants
title_sort	in vivo toxicity evaluation of ethanolic extract from two mangrove plants
publisher	Mazandaran University of Medical Sciences
publishDate	2021
url	https://doaj.org/article/78cd359f29c441c8802bfe935dd9bf32
work_keys_str_mv	AT ranabiswas invivotoxicityevaluationofethanolicextractfromtwomangroveplants AT mdabdullahalmunna invivotoxicityevaluationofethanolicextractfromtwomangroveplants AT mdmorsalinebillah invivotoxicityevaluationofethanolicextractfromtwomangroveplants AT mdemdadulislam invivotoxicityevaluationofethanolicextractfromtwomangroveplants AT kazimohammeddidarulislam invivotoxicityevaluationofethanolicextractfromtwomangroveplants AT afiyaaunjum invivotoxicityevaluationofethanolicextractfromtwomangroveplants
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In vivo Toxicity Evaluation of Ethanolic Extract From Two Mangrove Plants

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