Impaired cell surface expression of HLA-B antigens on mesenchymal stem cells and muscle cell progenitors.

HLA class-I expression is weak in embryonic stem cells but increases rapidly during lineage progression. It is unknown whether all three classical HLA class-I antigens follow the same developmental program. In the present study, we investigated allele-specific expression of HLA-A, -B, and -C at the...

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Autores principales:	Adiba Isa, Jan O Nehlin, Hardee J Sabir, Tom E Andersen, Michael Gaster, Moustapha Kassem, Torben Barington
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/78cf091d47624af88eee8f8156bd0a49
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spelling	oai:doaj.org-article:78cf091d47624af88eee8f8156bd0a492021-12-02T20:21:16ZImpaired cell surface expression of HLA-B antigens on mesenchymal stem cells and muscle cell progenitors.1932-620310.1371/journal.pone.0010900https://doaj.org/article/78cf091d47624af88eee8f8156bd0a492010-05-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20531935/?tool=EBIhttps://doaj.org/toc/1932-6203HLA class-I expression is weak in embryonic stem cells but increases rapidly during lineage progression. It is unknown whether all three classical HLA class-I antigens follow the same developmental program. In the present study, we investigated allele-specific expression of HLA-A, -B, and -C at the mRNA and protein levels on human mesenchymal stem cells from bone marrow and adipose tissue as well as striated muscle satellite cells and lymphocytes. Using multicolour flow cytometry, we found high cell surface expression of HLA-A on all stem cells and PBMC examined. Surprisingly, HLA-B was either undetectable or very weakly expressed on all stem cells protecting them from complement-dependent cytotoxicity (CDC) using relevant human anti-B and anti-Cw sera. IFNgamma stimulation for 48-72 h was required to induce full HLA-B protein expression. Quantitative real-time RT-PCR showed that IFNgamma induced a 9-42 fold increase of all six HLA-A,-B,-C gene transcripts. Interestingly, prior to stimulation, gene transcripts for all but two alleles were present in similar amounts suggesting that post-transcriptional mechanisms regulate the constitutive expression of HLA-A,-B, and -C. Locus-restricted expression of HLA-A, -B and -C challenges our current understanding of the function of these molecules as regulators of CD8(+) T-cell and NK-cell function and should lead to further inquiries into their expression on other cell types.Adiba IsaJan O NehlinHardee J SabirTom E AndersenMichael GasterMoustapha KassemTorben BaringtonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 5, p e10900 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Adiba Isa Jan O Nehlin Hardee J Sabir Tom E Andersen Michael Gaster Moustapha Kassem Torben Barington Impaired cell surface expression of HLA-B antigens on mesenchymal stem cells and muscle cell progenitors.
description	HLA class-I expression is weak in embryonic stem cells but increases rapidly during lineage progression. It is unknown whether all three classical HLA class-I antigens follow the same developmental program. In the present study, we investigated allele-specific expression of HLA-A, -B, and -C at the mRNA and protein levels on human mesenchymal stem cells from bone marrow and adipose tissue as well as striated muscle satellite cells and lymphocytes. Using multicolour flow cytometry, we found high cell surface expression of HLA-A on all stem cells and PBMC examined. Surprisingly, HLA-B was either undetectable or very weakly expressed on all stem cells protecting them from complement-dependent cytotoxicity (CDC) using relevant human anti-B and anti-Cw sera. IFNgamma stimulation for 48-72 h was required to induce full HLA-B protein expression. Quantitative real-time RT-PCR showed that IFNgamma induced a 9-42 fold increase of all six HLA-A,-B,-C gene transcripts. Interestingly, prior to stimulation, gene transcripts for all but two alleles were present in similar amounts suggesting that post-transcriptional mechanisms regulate the constitutive expression of HLA-A,-B, and -C. Locus-restricted expression of HLA-A, -B and -C challenges our current understanding of the function of these molecules as regulators of CD8(+) T-cell and NK-cell function and should lead to further inquiries into their expression on other cell types.
format	article
author	Adiba Isa Jan O Nehlin Hardee J Sabir Tom E Andersen Michael Gaster Moustapha Kassem Torben Barington
author_facet	Adiba Isa Jan O Nehlin Hardee J Sabir Tom E Andersen Michael Gaster Moustapha Kassem Torben Barington
author_sort	Adiba Isa
title	Impaired cell surface expression of HLA-B antigens on mesenchymal stem cells and muscle cell progenitors.
title_short	Impaired cell surface expression of HLA-B antigens on mesenchymal stem cells and muscle cell progenitors.
title_full	Impaired cell surface expression of HLA-B antigens on mesenchymal stem cells and muscle cell progenitors.
title_fullStr	Impaired cell surface expression of HLA-B antigens on mesenchymal stem cells and muscle cell progenitors.
title_full_unstemmed	Impaired cell surface expression of HLA-B antigens on mesenchymal stem cells and muscle cell progenitors.
title_sort	impaired cell surface expression of hla-b antigens on mesenchymal stem cells and muscle cell progenitors.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/78cf091d47624af88eee8f8156bd0a49
work_keys_str_mv	AT adibaisa impairedcellsurfaceexpressionofhlabantigensonmesenchymalstemcellsandmusclecellprogenitors AT janonehlin impairedcellsurfaceexpressionofhlabantigensonmesenchymalstemcellsandmusclecellprogenitors AT hardeejsabir impairedcellsurfaceexpressionofhlabantigensonmesenchymalstemcellsandmusclecellprogenitors AT tomeandersen impairedcellsurfaceexpressionofhlabantigensonmesenchymalstemcellsandmusclecellprogenitors AT michaelgaster impairedcellsurfaceexpressionofhlabantigensonmesenchymalstemcellsandmusclecellprogenitors AT moustaphakassem impairedcellsurfaceexpressionofhlabantigensonmesenchymalstemcellsandmusclecellprogenitors AT torbenbarington impairedcellsurfaceexpressionofhlabantigensonmesenchymalstemcellsandmusclecellprogenitors
_version_	1718374115137552384

Impaired cell surface expression of HLA-B antigens on mesenchymal stem cells and muscle cell progenitors.

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