Evaluation of the antibacterial activity of tilmicosin-SLN against Streptococcus agalactiae: in vitro and in vivo studies

Luyan Zhu,1 Xiaoxia Cao,1 Qinxin Xu,1 Jing Su,1 Xihe Li,2,3 Wenzhong Zhou1 1Department of Preventive Veterinary Medicine, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, People’s Republic of China; 2Research Center for Animal Genetic Resources of Mongolian...

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Autores principales:	Zhu L, Cao X, Xu Q, Su J, Li X, Zhou W
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	tilmicosin solid lipid nanoparticles Streptococcus agalactiae Mastitis Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/78d22701188c4f7aa540fc7916b91e45
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spelling	oai:doaj.org-article:78d22701188c4f7aa540fc7916b91e452021-12-02T00:40:30ZEvaluation of the antibacterial activity of tilmicosin-SLN against Streptococcus agalactiae: in vitro and in vivo studies1178-2013https://doaj.org/article/78d22701188c4f7aa540fc7916b91e452018-08-01T00:00:00Zhttps://www.dovepress.com/evaluation-of-the-antibacterial-activity-of-tilmicosin-sln-against-str-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Luyan Zhu,1 Xiaoxia Cao,1 Qinxin Xu,1 Jing Su,1 Xihe Li,2,3 Wenzhong Zhou1 1Department of Preventive Veterinary Medicine, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, People’s Republic of China; 2Research Center for Animal Genetic Resources of Mongolian Plateau School of Life Sciences, Inner Mongolia University, Hohhot, 010021, People’s Republic of China; 3Inner Mongolia Saikexing Institute of Breeding and Reproductive Biotechnology in Domestic Animals, Saikexing Breeding and Reproductive Biotechnology Co. Ltd., Hohhot, 011517, People’s Republic of China Background: Streptococcus and Staphylococcus are the major contagious organisms causing dairy cow mastitis. Our previous studies have demonstrated that solid lipid nanoparticles (SLNs) can effectively enhance the antimicrobial activity of tilmicosin against Staphylococcus. This study aimed to evaluate the antibacterial efficacy of tilmicosin-loaded SLN (Til-SLN) against Streptococcus agalactiae. Methods: Til-SLN was prepared using a hot homogenization and ultrasonication method as described previously. Til-SLN was labeled with rhodamine B for nanoparticle tracking. In vitro antibacterial experiments were carried out by broth dilution technique. Pharmacokinetics of the drug and distribution of the nanoparticles in mammary gland were studied after subcutaneous injection in Kunming mice. The therapeutic study was conducted in a mouse mastitis model infected with S. agalactiae. Results: The results showed that the diameter, polydispersity index, zeta potential, encapsulation efficiency, and loading capacity of the nanoparticles were not significantly affected by fluorescence labeling. Til-SLN showed a sustained and enhanced antibacterial activity in vitro. Til-SLN maintained a sustained drug concentration above 17 µg/g for at least 6 days in the mammary gland, as compared with only 3 days for the same amount of tilmicosin phosphate solution. The mean residence time and elimination half-life (T1/2) of Til-SLN were much longer than those of tilmicosin phosphate solution. Most of the nanoparticles remained at the injection site and a few were transferred to the mammary glands, indicating that the drug was slowly released at the injection site and then distributed to the mammary glands. SLN significantly enhanced the therapeutic efficacy of tilmicosin as determined by lower colony forming unit counts. Conclusion: These results demonstrate that SLN could effectively enhance the antibacterial activity of tilmicosin against Streptococcus. Keywords: tilmicosin, solid lipid nanoparticles, Streptococcus agalactiae, mastitisZhu LCao XXu QSu JLi XZhou WDove Medical Pressarticletilmicosinsolid lipid nanoparticlesStreptococcus agalactiaeMastitisMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 4747-4755 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	tilmicosin solid lipid nanoparticles Streptococcus agalactiae Mastitis Medicine (General) R5-920
spellingShingle	tilmicosin solid lipid nanoparticles Streptococcus agalactiae Mastitis Medicine (General) R5-920 Zhu L Cao X Xu Q Su J Li X Zhou W Evaluation of the antibacterial activity of tilmicosin-SLN against Streptococcus agalactiae: in vitro and in vivo studies
description	Luyan Zhu,1 Xiaoxia Cao,1 Qinxin Xu,1 Jing Su,1 Xihe Li,2,3 Wenzhong Zhou1 1Department of Preventive Veterinary Medicine, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, People’s Republic of China; 2Research Center for Animal Genetic Resources of Mongolian Plateau School of Life Sciences, Inner Mongolia University, Hohhot, 010021, People’s Republic of China; 3Inner Mongolia Saikexing Institute of Breeding and Reproductive Biotechnology in Domestic Animals, Saikexing Breeding and Reproductive Biotechnology Co. Ltd., Hohhot, 011517, People’s Republic of China Background: Streptococcus and Staphylococcus are the major contagious organisms causing dairy cow mastitis. Our previous studies have demonstrated that solid lipid nanoparticles (SLNs) can effectively enhance the antimicrobial activity of tilmicosin against Staphylococcus. This study aimed to evaluate the antibacterial efficacy of tilmicosin-loaded SLN (Til-SLN) against Streptococcus agalactiae. Methods: Til-SLN was prepared using a hot homogenization and ultrasonication method as described previously. Til-SLN was labeled with rhodamine B for nanoparticle tracking. In vitro antibacterial experiments were carried out by broth dilution technique. Pharmacokinetics of the drug and distribution of the nanoparticles in mammary gland were studied after subcutaneous injection in Kunming mice. The therapeutic study was conducted in a mouse mastitis model infected with S. agalactiae. Results: The results showed that the diameter, polydispersity index, zeta potential, encapsulation efficiency, and loading capacity of the nanoparticles were not significantly affected by fluorescence labeling. Til-SLN showed a sustained and enhanced antibacterial activity in vitro. Til-SLN maintained a sustained drug concentration above 17 µg/g for at least 6 days in the mammary gland, as compared with only 3 days for the same amount of tilmicosin phosphate solution. The mean residence time and elimination half-life (T1/2) of Til-SLN were much longer than those of tilmicosin phosphate solution. Most of the nanoparticles remained at the injection site and a few were transferred to the mammary glands, indicating that the drug was slowly released at the injection site and then distributed to the mammary glands. SLN significantly enhanced the therapeutic efficacy of tilmicosin as determined by lower colony forming unit counts. Conclusion: These results demonstrate that SLN could effectively enhance the antibacterial activity of tilmicosin against Streptococcus. Keywords: tilmicosin, solid lipid nanoparticles, Streptococcus agalactiae, mastitis
format	article
author	Zhu L Cao X Xu Q Su J Li X Zhou W
author_facet	Zhu L Cao X Xu Q Su J Li X Zhou W
author_sort	Zhu L
title	Evaluation of the antibacterial activity of tilmicosin-SLN against Streptococcus agalactiae: in vitro and in vivo studies
title_short	Evaluation of the antibacterial activity of tilmicosin-SLN against Streptococcus agalactiae: in vitro and in vivo studies
title_full	Evaluation of the antibacterial activity of tilmicosin-SLN against Streptococcus agalactiae: in vitro and in vivo studies
title_fullStr	Evaluation of the antibacterial activity of tilmicosin-SLN against Streptococcus agalactiae: in vitro and in vivo studies
title_full_unstemmed	Evaluation of the antibacterial activity of tilmicosin-SLN against Streptococcus agalactiae: in vitro and in vivo studies
title_sort	evaluation of the antibacterial activity of tilmicosin-sln against streptococcus agalactiae: in vitro and in vivo studies
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/78d22701188c4f7aa540fc7916b91e45
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Evaluation of the antibacterial activity of tilmicosin-SLN against Streptococcus agalactiae: in vitro and in vivo studies

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