Strain maps characterize the symmetry of convergence and extension patterns during zebrafish gastrulation

Abstract During gastrulation of the zebrafish embryo, the cap of blastoderm cells organizes into the axial body plan of the embryo with left–right symmetry and head–tail, dorsal–ventral polarities. Our labs have been interested in the mechanics of early development and have investigated whether thes...

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Autores principales:	Dipanjan Bhattacharya, Jun Zhong, Sahar Tavakoli, Alexandre Kabla, Paul Matsudaira
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/78d28d1de5274be8a614a4b4705eb4b1
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spelling	oai:doaj.org-article:78d28d1de5274be8a614a4b4705eb4b12021-12-02T17:17:39ZStrain maps characterize the symmetry of convergence and extension patterns during zebrafish gastrulation10.1038/s41598-021-98233-z2045-2322https://doaj.org/article/78d28d1de5274be8a614a4b4705eb4b12021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98233-zhttps://doaj.org/toc/2045-2322Abstract During gastrulation of the zebrafish embryo, the cap of blastoderm cells organizes into the axial body plan of the embryo with left–right symmetry and head–tail, dorsal–ventral polarities. Our labs have been interested in the mechanics of early development and have investigated whether these large-scale cell movements can be described as tissue-level mechanical strain by a tectonics-based approach. The first step is to image the positions of all nuclei from mid-epiboly to early segmentation by digital sheet light microscopy, organize the surface of the embryo into multi-cell spherical domains, construct velocity fields from the movements of these domains and extract strain rate maps from the change in density of the domains. During gastrulation, tensile/expansive and compressive strains in the axial and equatorial directions are detected as anterior and posterior expansion along the anterior–posterior axis and medial–lateral compression across the dorsal–ventral axis and corresponds to the well characterized morphological movements of convergence and extension. Following gastrulation strain is represented by localized medial expansion at the onset of segmentation and anterior expansion at the onset of neurulation. In addition to linear strain, symmetric patterns of rotation/curl are first detected in the animal hemispheres at mid-epiboly and then the vegetal hemispheres by the end of gastrulation. In embryos treated with C59, a Wnt inhibitor that inhibits head and tail extension, the axial extension and vegetal curl are absent. By analysing the temporal sequence of large-scale movements, deformations across the embryo can be attributed to a combination of epiboly and dorsal convergence-extension.Dipanjan BhattacharyaJun ZhongSahar TavakoliAlexandre KablaPaul MatsudairaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dipanjan Bhattacharya Jun Zhong Sahar Tavakoli Alexandre Kabla Paul Matsudaira Strain maps characterize the symmetry of convergence and extension patterns during zebrafish gastrulation
description	Abstract During gastrulation of the zebrafish embryo, the cap of blastoderm cells organizes into the axial body plan of the embryo with left–right symmetry and head–tail, dorsal–ventral polarities. Our labs have been interested in the mechanics of early development and have investigated whether these large-scale cell movements can be described as tissue-level mechanical strain by a tectonics-based approach. The first step is to image the positions of all nuclei from mid-epiboly to early segmentation by digital sheet light microscopy, organize the surface of the embryo into multi-cell spherical domains, construct velocity fields from the movements of these domains and extract strain rate maps from the change in density of the domains. During gastrulation, tensile/expansive and compressive strains in the axial and equatorial directions are detected as anterior and posterior expansion along the anterior–posterior axis and medial–lateral compression across the dorsal–ventral axis and corresponds to the well characterized morphological movements of convergence and extension. Following gastrulation strain is represented by localized medial expansion at the onset of segmentation and anterior expansion at the onset of neurulation. In addition to linear strain, symmetric patterns of rotation/curl are first detected in the animal hemispheres at mid-epiboly and then the vegetal hemispheres by the end of gastrulation. In embryos treated with C59, a Wnt inhibitor that inhibits head and tail extension, the axial extension and vegetal curl are absent. By analysing the temporal sequence of large-scale movements, deformations across the embryo can be attributed to a combination of epiboly and dorsal convergence-extension.
format	article
author	Dipanjan Bhattacharya Jun Zhong Sahar Tavakoli Alexandre Kabla Paul Matsudaira
author_facet	Dipanjan Bhattacharya Jun Zhong Sahar Tavakoli Alexandre Kabla Paul Matsudaira
author_sort	Dipanjan Bhattacharya
title	Strain maps characterize the symmetry of convergence and extension patterns during zebrafish gastrulation
title_short	Strain maps characterize the symmetry of convergence and extension patterns during zebrafish gastrulation
title_full	Strain maps characterize the symmetry of convergence and extension patterns during zebrafish gastrulation
title_fullStr	Strain maps characterize the symmetry of convergence and extension patterns during zebrafish gastrulation
title_full_unstemmed	Strain maps characterize the symmetry of convergence and extension patterns during zebrafish gastrulation
title_sort	strain maps characterize the symmetry of convergence and extension patterns during zebrafish gastrulation
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/78d28d1de5274be8a614a4b4705eb4b1
work_keys_str_mv	AT dipanjanbhattacharya strainmapscharacterizethesymmetryofconvergenceandextensionpatternsduringzebrafishgastrulation AT junzhong strainmapscharacterizethesymmetryofconvergenceandextensionpatternsduringzebrafishgastrulation AT sahartavakoli strainmapscharacterizethesymmetryofconvergenceandextensionpatternsduringzebrafishgastrulation AT alexandrekabla strainmapscharacterizethesymmetryofconvergenceandextensionpatternsduringzebrafishgastrulation AT paulmatsudaira strainmapscharacterizethesymmetryofconvergenceandextensionpatternsduringzebrafishgastrulation
_version_	1718381152605044736

Strain maps characterize the symmetry of convergence and extension patterns during zebrafish gastrulation

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