The characteristics of proteome and metabolome associated with contrasting sperm motility in goat seminal plasma

Abstract Sperm motility is an index tightly associated with male fertility. A close relationship between seminal plasma and sperm motility has been confirmed. This study was to assess the protein and metabolite profiles of seminal plasma obtained from adult goats with high or low sperm motility usin...

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Autores principales:	Baoyu Jia, Jiachong Liang, Chunrong Lv, Sameeullah Memon, Yi Fang, Guoquan Wu, Guobo Quan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/78d367290a884897967543c33a8cda44
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spelling	oai:doaj.org-article:78d367290a884897967543c33a8cda442021-12-02T16:06:44ZThe characteristics of proteome and metabolome associated with contrasting sperm motility in goat seminal plasma10.1038/s41598-021-95138-92045-2322https://doaj.org/article/78d367290a884897967543c33a8cda442021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95138-9https://doaj.org/toc/2045-2322Abstract Sperm motility is an index tightly associated with male fertility. A close relationship between seminal plasma and sperm motility has been confirmed. This study was to assess the protein and metabolite profiles of seminal plasma obtained from adult goats with high or low sperm motility using the proteomic and metabolomic strategies. In total, 2098 proteins were found. 449 differentially abundant proteins (DAPs) were identified, and 175 DAPs were enriched in the high motility group. The obtained DAPs primarily exist in cytoplasma and extra-cellular portion. The Gene Ontology enrichment analysis demonstrated the main functional roles of these DAPs in regulating biological process, metabolic process of organic substances, cellular-metabolic process, primary-metabolic process, metabolic process of nitrogen compounds, etc. Additionally, the Kyoto-Encyclopedia of Genes and Genomes (KEGG) analysis revealed that these DAPs were primarily involved in phosphatidylinositol signaling system, salivary secretion, proteasome, apoptosis, mitophagy-animal, etc. Aided by the parallel reaction monitoring technology, the abundance changing pattern of 19 selected DAPs was consistent with that of the corresponding proteins obtained by TMT. A total of 4603 metabolites were identified in seminal plasma. 1857 differential metabolites were found between the high motility group and the low motility group, and 999 metabolites were up-regulated in the high motility group. The KEGG analysis demonstrated the primary involvement of the differential metabolites in metabolic and synthetic activities. In conclusion, we first established the proteome and metabolome databank of goat seminal plasma, detecting some proteins and metabolites which may affect sperm motility. This study will be valuable for understanding mechanisms leading to poor sperm motility.Baoyu JiaJiachong LiangChunrong LvSameeullah MemonYi FangGuoquan WuGuobo QuanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Baoyu Jia Jiachong Liang Chunrong Lv Sameeullah Memon Yi Fang Guoquan Wu Guobo Quan The characteristics of proteome and metabolome associated with contrasting sperm motility in goat seminal plasma
description	Abstract Sperm motility is an index tightly associated with male fertility. A close relationship between seminal plasma and sperm motility has been confirmed. This study was to assess the protein and metabolite profiles of seminal plasma obtained from adult goats with high or low sperm motility using the proteomic and metabolomic strategies. In total, 2098 proteins were found. 449 differentially abundant proteins (DAPs) were identified, and 175 DAPs were enriched in the high motility group. The obtained DAPs primarily exist in cytoplasma and extra-cellular portion. The Gene Ontology enrichment analysis demonstrated the main functional roles of these DAPs in regulating biological process, metabolic process of organic substances, cellular-metabolic process, primary-metabolic process, metabolic process of nitrogen compounds, etc. Additionally, the Kyoto-Encyclopedia of Genes and Genomes (KEGG) analysis revealed that these DAPs were primarily involved in phosphatidylinositol signaling system, salivary secretion, proteasome, apoptosis, mitophagy-animal, etc. Aided by the parallel reaction monitoring technology, the abundance changing pattern of 19 selected DAPs was consistent with that of the corresponding proteins obtained by TMT. A total of 4603 metabolites were identified in seminal plasma. 1857 differential metabolites were found between the high motility group and the low motility group, and 999 metabolites were up-regulated in the high motility group. The KEGG analysis demonstrated the primary involvement of the differential metabolites in metabolic and synthetic activities. In conclusion, we first established the proteome and metabolome databank of goat seminal plasma, detecting some proteins and metabolites which may affect sperm motility. This study will be valuable for understanding mechanisms leading to poor sperm motility.
format	article
author	Baoyu Jia Jiachong Liang Chunrong Lv Sameeullah Memon Yi Fang Guoquan Wu Guobo Quan
author_facet	Baoyu Jia Jiachong Liang Chunrong Lv Sameeullah Memon Yi Fang Guoquan Wu Guobo Quan
author_sort	Baoyu Jia
title	The characteristics of proteome and metabolome associated with contrasting sperm motility in goat seminal plasma
title_short	The characteristics of proteome and metabolome associated with contrasting sperm motility in goat seminal plasma
title_full	The characteristics of proteome and metabolome associated with contrasting sperm motility in goat seminal plasma
title_fullStr	The characteristics of proteome and metabolome associated with contrasting sperm motility in goat seminal plasma
title_full_unstemmed	The characteristics of proteome and metabolome associated with contrasting sperm motility in goat seminal plasma
title_sort	characteristics of proteome and metabolome associated with contrasting sperm motility in goat seminal plasma
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/78d367290a884897967543c33a8cda44
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The characteristics of proteome and metabolome associated with contrasting sperm motility in goat seminal plasma

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