Structure-based dynamic analysis of the glycine cleavage system suggests key residues for control of a key reaction step

Han Zhang et al. report molecular dynamics simulations and mutational analysis of a key process of the E. coli glycine cleavage system, an important enzyme complex in C1 metabolism. They identify a key amino acid residue controlling the release of the swinging aminomethyl lipoate arm and increase th...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Han Zhang, Yuchen Li, Jinglei Nie, Jie Ren, An-Ping Zeng
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/78e895b2a82e49aea72b3a9bd99928e3
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:78e895b2a82e49aea72b3a9bd99928e3
record_format	dspace
spelling	oai:doaj.org-article:78e895b2a82e49aea72b3a9bd99928e32021-12-02T11:43:43ZStructure-based dynamic analysis of the glycine cleavage system suggests key residues for control of a key reaction step10.1038/s42003-020-01401-62399-3642https://doaj.org/article/78e895b2a82e49aea72b3a9bd99928e32020-12-01T00:00:00Zhttps://doi.org/10.1038/s42003-020-01401-6https://doaj.org/toc/2399-3642Han Zhang et al. report molecular dynamics simulations and mutational analysis of a key process of the E. coli glycine cleavage system, an important enzyme complex in C1 metabolism. They identify a key amino acid residue controlling the release of the swinging aminomethyl lipoate arm and increase the overall reaction rate of glycine cleavage by more than twice, providing a strategy for manipulating this reaction system for use in synthetic biology.Han ZhangYuchen LiJinglei NieJie RenAn-Ping ZengNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 3, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Han Zhang Yuchen Li Jinglei Nie Jie Ren An-Ping Zeng Structure-based dynamic analysis of the glycine cleavage system suggests key residues for control of a key reaction step
description	Han Zhang et al. report molecular dynamics simulations and mutational analysis of a key process of the E. coli glycine cleavage system, an important enzyme complex in C1 metabolism. They identify a key amino acid residue controlling the release of the swinging aminomethyl lipoate arm and increase the overall reaction rate of glycine cleavage by more than twice, providing a strategy for manipulating this reaction system for use in synthetic biology.
format	article
author	Han Zhang Yuchen Li Jinglei Nie Jie Ren An-Ping Zeng
author_facet	Han Zhang Yuchen Li Jinglei Nie Jie Ren An-Ping Zeng
author_sort	Han Zhang
title	Structure-based dynamic analysis of the glycine cleavage system suggests key residues for control of a key reaction step
title_short	Structure-based dynamic analysis of the glycine cleavage system suggests key residues for control of a key reaction step
title_full	Structure-based dynamic analysis of the glycine cleavage system suggests key residues for control of a key reaction step
title_fullStr	Structure-based dynamic analysis of the glycine cleavage system suggests key residues for control of a key reaction step
title_full_unstemmed	Structure-based dynamic analysis of the glycine cleavage system suggests key residues for control of a key reaction step
title_sort	structure-based dynamic analysis of the glycine cleavage system suggests key residues for control of a key reaction step
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/78e895b2a82e49aea72b3a9bd99928e3
work_keys_str_mv	AT hanzhang structurebaseddynamicanalysisoftheglycinecleavagesystemsuggestskeyresiduesforcontrolofakeyreactionstep AT yuchenli structurebaseddynamicanalysisoftheglycinecleavagesystemsuggestskeyresiduesforcontrolofakeyreactionstep AT jingleinie structurebaseddynamicanalysisoftheglycinecleavagesystemsuggestskeyresiduesforcontrolofakeyreactionstep AT jieren structurebaseddynamicanalysisoftheglycinecleavagesystemsuggestskeyresiduesforcontrolofakeyreactionstep AT anpingzeng structurebaseddynamicanalysisoftheglycinecleavagesystemsuggestskeyresiduesforcontrolofakeyreactionstep
_version_	1718395351231102976

Structure-based dynamic analysis of the glycine cleavage system suggests key residues for control of a key reaction step

Ejemplares similares