Serum microRNA-1 and microRNA-133a levels reflect myocardial steatosis in uncomplicated type 2 diabetes

Abstract Using in vitro, in vivo and patient-based approaches, we investigated the potential of circulating microRNAs (miRNAs) as surrogate biomarkers of myocardial steatosis, a hallmark of diabetic cardiomyopathy. We analysed the cardiomyocyte-enriched miRNA signature in serum from patients with we...

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Autores principales:	D. de Gonzalo-Calvo, R. W. van der Meer, L. J. Rijzewijk, J. W. A. Smit, E. Revuelta-Lopez, L. Nasarre, J. C. Escola-Gil, H. J. Lamb, V. Llorente-Cortes
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/78f141abab4d47499cb088d493932491
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spelling	oai:doaj.org-article:78f141abab4d47499cb088d4939324912021-12-02T15:05:18ZSerum microRNA-1 and microRNA-133a levels reflect myocardial steatosis in uncomplicated type 2 diabetes10.1038/s41598-017-00070-62045-2322https://doaj.org/article/78f141abab4d47499cb088d4939324912017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00070-6https://doaj.org/toc/2045-2322Abstract Using in vitro, in vivo and patient-based approaches, we investigated the potential of circulating microRNAs (miRNAs) as surrogate biomarkers of myocardial steatosis, a hallmark of diabetic cardiomyopathy. We analysed the cardiomyocyte-enriched miRNA signature in serum from patients with well-controlled type 2 diabetes and with verified absence of structural heart disease or inducible ischemia, and control volunteers of the same age range and BMI (N = 86), in serum from a high-fat diet-fed murine model, and in exosomes from lipid-loaded HL-1 cardiomyocytes. Circulating miR-1 and miR-133a levels were robustly associated with myocardial steatosis in type 2 diabetes patients, independently of confounding factors in both linear and logistic regression analyses (P < 0.050 for all models). Similar to myocardial steatosis, miR-133a levels were increased in type 2 diabetes patients as compared with healthy subjects (P < 0.050). Circulating miR-1 and miR-133a levels were significantly elevated in high-fat diet-fed mice (P < 0.050), which showed higher myocardial steatosis, as compared with control animals. miR-1 and miR-133a levels were higher in exosomes released from lipid-loaded HL-1 cardiomyocytes (P < 0.050). Circulating miR-1 and miR-133a are independent predictors of myocardial steatosis. Our results highlight the value of circulating miRNAs as diagnostic tools for subclinical diabetic cardiomyopathy.D. de Gonzalo-CalvoR. W. van der MeerL. J. RijzewijkJ. W. A. SmitE. Revuelta-LopezL. NasarreJ. C. Escola-GilH. J. LambV. Llorente-CortesNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q D. de Gonzalo-Calvo R. W. van der Meer L. J. Rijzewijk J. W. A. Smit E. Revuelta-Lopez L. Nasarre J. C. Escola-Gil H. J. Lamb V. Llorente-Cortes Serum microRNA-1 and microRNA-133a levels reflect myocardial steatosis in uncomplicated type 2 diabetes
description	Abstract Using in vitro, in vivo and patient-based approaches, we investigated the potential of circulating microRNAs (miRNAs) as surrogate biomarkers of myocardial steatosis, a hallmark of diabetic cardiomyopathy. We analysed the cardiomyocyte-enriched miRNA signature in serum from patients with well-controlled type 2 diabetes and with verified absence of structural heart disease or inducible ischemia, and control volunteers of the same age range and BMI (N = 86), in serum from a high-fat diet-fed murine model, and in exosomes from lipid-loaded HL-1 cardiomyocytes. Circulating miR-1 and miR-133a levels were robustly associated with myocardial steatosis in type 2 diabetes patients, independently of confounding factors in both linear and logistic regression analyses (P < 0.050 for all models). Similar to myocardial steatosis, miR-133a levels were increased in type 2 diabetes patients as compared with healthy subjects (P < 0.050). Circulating miR-1 and miR-133a levels were significantly elevated in high-fat diet-fed mice (P < 0.050), which showed higher myocardial steatosis, as compared with control animals. miR-1 and miR-133a levels were higher in exosomes released from lipid-loaded HL-1 cardiomyocytes (P < 0.050). Circulating miR-1 and miR-133a are independent predictors of myocardial steatosis. Our results highlight the value of circulating miRNAs as diagnostic tools for subclinical diabetic cardiomyopathy.
format	article
author	D. de Gonzalo-Calvo R. W. van der Meer L. J. Rijzewijk J. W. A. Smit E. Revuelta-Lopez L. Nasarre J. C. Escola-Gil H. J. Lamb V. Llorente-Cortes
author_facet	D. de Gonzalo-Calvo R. W. van der Meer L. J. Rijzewijk J. W. A. Smit E. Revuelta-Lopez L. Nasarre J. C. Escola-Gil H. J. Lamb V. Llorente-Cortes
author_sort	D. de Gonzalo-Calvo
title	Serum microRNA-1 and microRNA-133a levels reflect myocardial steatosis in uncomplicated type 2 diabetes
title_short	Serum microRNA-1 and microRNA-133a levels reflect myocardial steatosis in uncomplicated type 2 diabetes
title_full	Serum microRNA-1 and microRNA-133a levels reflect myocardial steatosis in uncomplicated type 2 diabetes
title_fullStr	Serum microRNA-1 and microRNA-133a levels reflect myocardial steatosis in uncomplicated type 2 diabetes
title_full_unstemmed	Serum microRNA-1 and microRNA-133a levels reflect myocardial steatosis in uncomplicated type 2 diabetes
title_sort	serum microrna-1 and microrna-133a levels reflect myocardial steatosis in uncomplicated type 2 diabetes
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/78f141abab4d47499cb088d493932491
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Serum microRNA-1 and microRNA-133a levels reflect myocardial steatosis in uncomplicated type 2 diabetes

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