Within-wing isotopic (δ2H, δ13C, δ15N) variation of monarch butterflies: implications for studies of migratory origins and diet

Increasingly, stable isotope measurements are being used to assign individuals to broad geographic origins based on established relationships between animal tissues and tissue-specific isoscapes. In particular, the eastern North American population of the monarch butterfly (Danaus plexippus) has bee...

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Autores principales:	Hobson Keith A., Plint Tessa, Serrano Eligio García, Alvarez Xiomara Mora, Ramirez Isabel, Longstaffe Fred J.
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2017
Materias:	carbon-13 deuterium isoscapes migration monarch butterfly nitrogen-15 stable isotopes Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/78f1434becb547a99b6265bc2636e6dd
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spelling	oai:doaj.org-article:78f1434becb547a99b6265bc2636e6dd2021-12-02T19:11:09ZWithin-wing isotopic (δ2H, δ13C, δ15N) variation of monarch butterflies: implications for studies of migratory origins and diet2084-883810.1515/ami-2017-0002https://doaj.org/article/78f1434becb547a99b6265bc2636e6dd2017-02-01T00:00:00Zhttps://doi.org/10.1515/ami-2017-0002https://doaj.org/toc/2084-8838Increasingly, stable isotope measurements are being used to assign individuals to broad geographic origins based on established relationships between animal tissues and tissue-specific isoscapes. In particular, the eastern North American population of the monarch butterfly (Danaus plexippus) has been the subject of several studies using established δ2H and δ13C wingtissue isoscapes to infer natal origins of migrating and overwintering individuals. However, there has been no study investigating potential variance that can derive from subsampling different regions of the wings, especially those regions differing in pigmentation (orange versus black). Within-wing isotopic (δ2H, δ13C, δ15N) variance of 40 monarch butterflies collected from natural overwinter mortality on Mexican roost sites were split evenly into two groups: unwashed samples and those washed in a 2:1 chloroform:methanol solvent. Isotopic variance in δ2H and δ13C was related to pigment (within-wing range 5‰ and 0.5‰, respectively), but not region of subsampling. This variance was reduced 3 to 4 fold through solvent washing that removed pigmented surface scales and any adhered oils. Wing δ15N was similarly influenced by pigment (range 0.3‰), but this effect was not reduced through washing. We recommend future isotopic studies of monarchs and other butterflies for migration research to use the same region for subsampling consistently and to wash samples with solvent to reduce isotopic variance related to uncontrolled variance in discrimination (δ2H, δ13C, δ15N) and/or adsorbed water vapor (δ2H). These data also need to be included in description of methods.Hobson Keith A.Plint TessaSerrano Eligio GarcíaAlvarez Xiomara MoraRamirez IsabelLongstaffe Fred J.De Gruyterarticlecarbon-13deuteriumisoscapesmigrationmonarch butterflynitrogen-15stable isotopesBiology (General)QH301-705.5ENAnimal Migration, Vol 4, Iss 1, Pp 8-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	carbon-13 deuterium isoscapes migration monarch butterfly nitrogen-15 stable isotopes Biology (General) QH301-705.5
spellingShingle	carbon-13 deuterium isoscapes migration monarch butterfly nitrogen-15 stable isotopes Biology (General) QH301-705.5 Hobson Keith A. Plint Tessa Serrano Eligio García Alvarez Xiomara Mora Ramirez Isabel Longstaffe Fred J. Within-wing isotopic (δ2H, δ13C, δ15N) variation of monarch butterflies: implications for studies of migratory origins and diet
description	Increasingly, stable isotope measurements are being used to assign individuals to broad geographic origins based on established relationships between animal tissues and tissue-specific isoscapes. In particular, the eastern North American population of the monarch butterfly (Danaus plexippus) has been the subject of several studies using established δ2H and δ13C wingtissue isoscapes to infer natal origins of migrating and overwintering individuals. However, there has been no study investigating potential variance that can derive from subsampling different regions of the wings, especially those regions differing in pigmentation (orange versus black). Within-wing isotopic (δ2H, δ13C, δ15N) variance of 40 monarch butterflies collected from natural overwinter mortality on Mexican roost sites were split evenly into two groups: unwashed samples and those washed in a 2:1 chloroform:methanol solvent. Isotopic variance in δ2H and δ13C was related to pigment (within-wing range 5‰ and 0.5‰, respectively), but not region of subsampling. This variance was reduced 3 to 4 fold through solvent washing that removed pigmented surface scales and any adhered oils. Wing δ15N was similarly influenced by pigment (range 0.3‰), but this effect was not reduced through washing. We recommend future isotopic studies of monarchs and other butterflies for migration research to use the same region for subsampling consistently and to wash samples with solvent to reduce isotopic variance related to uncontrolled variance in discrimination (δ2H, δ13C, δ15N) and/or adsorbed water vapor (δ2H). These data also need to be included in description of methods.
format	article
author	Hobson Keith A. Plint Tessa Serrano Eligio García Alvarez Xiomara Mora Ramirez Isabel Longstaffe Fred J.
author_facet	Hobson Keith A. Plint Tessa Serrano Eligio García Alvarez Xiomara Mora Ramirez Isabel Longstaffe Fred J.
author_sort	Hobson Keith A.
title	Within-wing isotopic (δ2H, δ13C, δ15N) variation of monarch butterflies: implications for studies of migratory origins and diet
title_short	Within-wing isotopic (δ2H, δ13C, δ15N) variation of monarch butterflies: implications for studies of migratory origins and diet
title_full	Within-wing isotopic (δ2H, δ13C, δ15N) variation of monarch butterflies: implications for studies of migratory origins and diet
title_fullStr	Within-wing isotopic (δ2H, δ13C, δ15N) variation of monarch butterflies: implications for studies of migratory origins and diet
title_full_unstemmed	Within-wing isotopic (δ2H, δ13C, δ15N) variation of monarch butterflies: implications for studies of migratory origins and diet
title_sort	within-wing isotopic (δ2h, δ13c, δ15n) variation of monarch butterflies: implications for studies of migratory origins and diet
publisher	De Gruyter
publishDate	2017
url	https://doaj.org/article/78f1434becb547a99b6265bc2636e6dd
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Within-wing isotopic (δ2H, δ13C, δ15N) variation of monarch butterflies: implications for studies of migratory origins and diet

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