Scopolamine promotes neuroinflammation and delirium-like neuropsychiatric disorder in mice

Abstract Postoperative delirium is a common neuropsychiatric syndrome resulting a high postsurgical mortality rate and decline in postdischarge function. Extensive research has been performed on both human and animal delirium-like models due to their clinical significance, focusing on systematic inf...

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Autores principales:	So Yeong Cheon, Bon-Nyeo Koo, So Yeon Kim, Eun Hee Kam, Junhyun Nam, Eun Jung Kim
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/78f9591f14b84a849888a20d6636cc54
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spelling	oai:doaj.org-article:78f9591f14b84a849888a20d6636cc542021-12-02T18:03:26ZScopolamine promotes neuroinflammation and delirium-like neuropsychiatric disorder in mice10.1038/s41598-021-87790-y2045-2322https://doaj.org/article/78f9591f14b84a849888a20d6636cc542021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87790-yhttps://doaj.org/toc/2045-2322Abstract Postoperative delirium is a common neuropsychiatric syndrome resulting a high postsurgical mortality rate and decline in postdischarge function. Extensive research has been performed on both human and animal delirium-like models due to their clinical significance, focusing on systematic inflammation and consequent neuroinflammation playing a key role in the pathogenesis of postoperative cognitive dysfunctions. Since animal models are widely utilized for pathophysiological study of neuropsychiatric disorders, this study aimed at examining the validity of the scopolamine-induced delirium-like mice model with respect to the neuroinflammatory hypothesis of delirium. Male C57BL/6 mice were treated with intraperitoneal scopolamine (2 mg/kg). Neurobehavioral tests were performed to evaluate the changes in cognitive functions, including learning and memory, and the level of anxiety after surgery or scopolamine treatment. The levels of pro-inflammatory cytokines (IL-1β, IL-18, and TNF-α) and inflammasome components (NLRP3, ASC, and caspase-1) in different brain regions were measured. Gene expression profiles were also examined using whole-genome RNA sequencing analyses to compare gene expression patterns of different mice models. Scopolamine treatment showed significant increase in the level of anxiety and impairments in memory and cognitive function associated with increased level of pro-inflammatory cytokines and NLRP3 inflammasome components. Genetic analysis confirmed the different expression patterns of genes involved in immune response and inflammation and those related with the development of the nervous system in both surgery and scopolamine-induced mice models. The scopolamine-induced delirium-like mice model successfully showed that analogous neuropsychiatric changes coincides with the neuroinflammatory hypothesis for pathogenesis of delirium.So Yeong CheonBon-Nyeo KooSo Yeon KimEun Hee KamJunhyun NamEun Jung KimNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q So Yeong Cheon Bon-Nyeo Koo So Yeon Kim Eun Hee Kam Junhyun Nam Eun Jung Kim Scopolamine promotes neuroinflammation and delirium-like neuropsychiatric disorder in mice
description	Abstract Postoperative delirium is a common neuropsychiatric syndrome resulting a high postsurgical mortality rate and decline in postdischarge function. Extensive research has been performed on both human and animal delirium-like models due to their clinical significance, focusing on systematic inflammation and consequent neuroinflammation playing a key role in the pathogenesis of postoperative cognitive dysfunctions. Since animal models are widely utilized for pathophysiological study of neuropsychiatric disorders, this study aimed at examining the validity of the scopolamine-induced delirium-like mice model with respect to the neuroinflammatory hypothesis of delirium. Male C57BL/6 mice were treated with intraperitoneal scopolamine (2 mg/kg). Neurobehavioral tests were performed to evaluate the changes in cognitive functions, including learning and memory, and the level of anxiety after surgery or scopolamine treatment. The levels of pro-inflammatory cytokines (IL-1β, IL-18, and TNF-α) and inflammasome components (NLRP3, ASC, and caspase-1) in different brain regions were measured. Gene expression profiles were also examined using whole-genome RNA sequencing analyses to compare gene expression patterns of different mice models. Scopolamine treatment showed significant increase in the level of anxiety and impairments in memory and cognitive function associated with increased level of pro-inflammatory cytokines and NLRP3 inflammasome components. Genetic analysis confirmed the different expression patterns of genes involved in immune response and inflammation and those related with the development of the nervous system in both surgery and scopolamine-induced mice models. The scopolamine-induced delirium-like mice model successfully showed that analogous neuropsychiatric changes coincides with the neuroinflammatory hypothesis for pathogenesis of delirium.
format	article
author	So Yeong Cheon Bon-Nyeo Koo So Yeon Kim Eun Hee Kam Junhyun Nam Eun Jung Kim
author_facet	So Yeong Cheon Bon-Nyeo Koo So Yeon Kim Eun Hee Kam Junhyun Nam Eun Jung Kim
author_sort	So Yeong Cheon
title	Scopolamine promotes neuroinflammation and delirium-like neuropsychiatric disorder in mice
title_short	Scopolamine promotes neuroinflammation and delirium-like neuropsychiatric disorder in mice
title_full	Scopolamine promotes neuroinflammation and delirium-like neuropsychiatric disorder in mice
title_fullStr	Scopolamine promotes neuroinflammation and delirium-like neuropsychiatric disorder in mice
title_full_unstemmed	Scopolamine promotes neuroinflammation and delirium-like neuropsychiatric disorder in mice
title_sort	scopolamine promotes neuroinflammation and delirium-like neuropsychiatric disorder in mice
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/78f9591f14b84a849888a20d6636cc54
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Scopolamine promotes neuroinflammation and delirium-like neuropsychiatric disorder in mice

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