β2-adrenergic receptor regulates ER-mitochondria contacts

Abstract Interactions between the endoplasmic reticulum (ER) and mitochondria (Mito) are crucial for many cellular functions, and their interaction levels change dynamically depending on the cellular environment. Little is known about how the interactions between these organelles are regulated withi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Youngshin Lim, Il-Taeg Cho, Helmut G. Rennke, Ginam Cho
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/78fa7932c9b64ab09ce7916196309add
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:78fa7932c9b64ab09ce7916196309add
record_format	dspace
spelling	oai:doaj.org-article:78fa7932c9b64ab09ce7916196309add2021-11-08T10:46:17Zβ2-adrenergic receptor regulates ER-mitochondria contacts10.1038/s41598-021-00801-w2045-2322https://doaj.org/article/78fa7932c9b64ab09ce7916196309add2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-00801-whttps://doaj.org/toc/2045-2322Abstract Interactions between the endoplasmic reticulum (ER) and mitochondria (Mito) are crucial for many cellular functions, and their interaction levels change dynamically depending on the cellular environment. Little is known about how the interactions between these organelles are regulated within the cell. Here we screened a compound library to identify chemical modulators for ER-Mito contacts in HEK293T cells. Multiple agonists of G-protein coupled receptors (GPCRs), beta-adrenergic receptors (β-ARs) in particular, scored in this screen. Analyses in multiple orthogonal assays validated that β2-AR activation promotes physical and functional interactions between the two organelles. Furthermore, we have elucidated potential downstream effectors mediating β2-AR-induced ER-Mito contacts. Together our study identifies β2-AR signaling as an important regulatory pathway for ER-Mito coupling and highlights the role of these contacts in responding to physiological demands or stresses.Youngshin LimIl-Taeg ChoHelmut G. RennkeGinam ChoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Youngshin Lim Il-Taeg Cho Helmut G. Rennke Ginam Cho β2-adrenergic receptor regulates ER-mitochondria contacts
description	Abstract Interactions between the endoplasmic reticulum (ER) and mitochondria (Mito) are crucial for many cellular functions, and their interaction levels change dynamically depending on the cellular environment. Little is known about how the interactions between these organelles are regulated within the cell. Here we screened a compound library to identify chemical modulators for ER-Mito contacts in HEK293T cells. Multiple agonists of G-protein coupled receptors (GPCRs), beta-adrenergic receptors (β-ARs) in particular, scored in this screen. Analyses in multiple orthogonal assays validated that β2-AR activation promotes physical and functional interactions between the two organelles. Furthermore, we have elucidated potential downstream effectors mediating β2-AR-induced ER-Mito contacts. Together our study identifies β2-AR signaling as an important regulatory pathway for ER-Mito coupling and highlights the role of these contacts in responding to physiological demands or stresses.
format	article
author	Youngshin Lim Il-Taeg Cho Helmut G. Rennke Ginam Cho
author_facet	Youngshin Lim Il-Taeg Cho Helmut G. Rennke Ginam Cho
author_sort	Youngshin Lim
title	β2-adrenergic receptor regulates ER-mitochondria contacts
title_short	β2-adrenergic receptor regulates ER-mitochondria contacts
title_full	β2-adrenergic receptor regulates ER-mitochondria contacts
title_fullStr	β2-adrenergic receptor regulates ER-mitochondria contacts
title_full_unstemmed	β2-adrenergic receptor regulates ER-mitochondria contacts
title_sort	β2-adrenergic receptor regulates er-mitochondria contacts
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/78fa7932c9b64ab09ce7916196309add
work_keys_str_mv	AT youngshinlim b2adrenergicreceptorregulatesermitochondriacontacts AT iltaegcho b2adrenergicreceptorregulatesermitochondriacontacts AT helmutgrennke b2adrenergicreceptorregulatesermitochondriacontacts AT ginamcho b2adrenergicreceptorregulatesermitochondriacontacts
_version_	1718442630045499392

β2-adrenergic receptor regulates ER-mitochondria contacts

Ejemplares similares