Diagnostic Performance of Ex Vivo Fluorescence Confocal Microscopy in the Assessment of Diagnostic Biopsies of the Prostate

Background: Fluorescence confocal microscopy (FCM) is a novel micro-imaging technique providing optical sections of examined tissue. The method has been well established for the diagnosis of tumors in dermatological specimens. Methods: We compare intraoperative diagnoses of the real-time application...

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Autores principales:	Ulf Titze, Torsten Hansen, Christoph Brochhausen, Barbara Titze, Birte Schulz, Alfons Gunnemann, Bernardo Rocco, Karl-Dietrich Sievert
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	confocal microscopy prostate cancer digital pathology Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/78fcfef9fc2241e686c7f6b9a4cc9baa
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spelling	oai:doaj.org-article:78fcfef9fc2241e686c7f6b9a4cc9baa2021-11-25T17:02:38ZDiagnostic Performance of Ex Vivo Fluorescence Confocal Microscopy in the Assessment of Diagnostic Biopsies of the Prostate10.3390/cancers132256852072-6694https://doaj.org/article/78fcfef9fc2241e686c7f6b9a4cc9baa2021-11-01T00:00:00Zhttps://www.mdpi.com/2072-6694/13/22/5685https://doaj.org/toc/2072-6694Background: Fluorescence confocal microscopy (FCM) is a novel micro-imaging technique providing optical sections of examined tissue. The method has been well established for the diagnosis of tumors in dermatological specimens. Methods: We compare intraoperative diagnoses of the real-time application of FCM in pre-therapeutic prostate biopsies (35 patients, total number of biopsy specimens: n = 438) with the findings of conventional histology. Results: Prostate carcinoma was reliably diagnosed in all patients. Depending on scan quality and experience of the examiner, smaller lesions of well differentiated carcinoma (ISUP1) could not be consistently differentiated from reactive changes. Furthermore, in some cases there was difficulty to distinguish ISUP grade 2 from ISUP grade 1 tumors. ISUP grades 3–5 were reliably detected in FCM. Conclusions: Despite some limitations, FCM seems to be an effective tool for the timely assessment of prostate biopsies enabling reliable diagnosis of prostate cancer in patients requiring therapy.Ulf TitzeTorsten HansenChristoph BrochhausenBarbara TitzeBirte SchulzAlfons GunnemannBernardo RoccoKarl-Dietrich SievertMDPI AGarticleconfocal microscopyprostate cancerdigital pathologyNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENCancers, Vol 13, Iss 5685, p 5685 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	confocal microscopy prostate cancer digital pathology Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	confocal microscopy prostate cancer digital pathology Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Ulf Titze Torsten Hansen Christoph Brochhausen Barbara Titze Birte Schulz Alfons Gunnemann Bernardo Rocco Karl-Dietrich Sievert Diagnostic Performance of Ex Vivo Fluorescence Confocal Microscopy in the Assessment of Diagnostic Biopsies of the Prostate
description	Background: Fluorescence confocal microscopy (FCM) is a novel micro-imaging technique providing optical sections of examined tissue. The method has been well established for the diagnosis of tumors in dermatological specimens. Methods: We compare intraoperative diagnoses of the real-time application of FCM in pre-therapeutic prostate biopsies (35 patients, total number of biopsy specimens: n = 438) with the findings of conventional histology. Results: Prostate carcinoma was reliably diagnosed in all patients. Depending on scan quality and experience of the examiner, smaller lesions of well differentiated carcinoma (ISUP1) could not be consistently differentiated from reactive changes. Furthermore, in some cases there was difficulty to distinguish ISUP grade 2 from ISUP grade 1 tumors. ISUP grades 3–5 were reliably detected in FCM. Conclusions: Despite some limitations, FCM seems to be an effective tool for the timely assessment of prostate biopsies enabling reliable diagnosis of prostate cancer in patients requiring therapy.
format	article
author	Ulf Titze Torsten Hansen Christoph Brochhausen Barbara Titze Birte Schulz Alfons Gunnemann Bernardo Rocco Karl-Dietrich Sievert
author_facet	Ulf Titze Torsten Hansen Christoph Brochhausen Barbara Titze Birte Schulz Alfons Gunnemann Bernardo Rocco Karl-Dietrich Sievert
author_sort	Ulf Titze
title	Diagnostic Performance of Ex Vivo Fluorescence Confocal Microscopy in the Assessment of Diagnostic Biopsies of the Prostate
title_short	Diagnostic Performance of Ex Vivo Fluorescence Confocal Microscopy in the Assessment of Diagnostic Biopsies of the Prostate
title_full	Diagnostic Performance of Ex Vivo Fluorescence Confocal Microscopy in the Assessment of Diagnostic Biopsies of the Prostate
title_fullStr	Diagnostic Performance of Ex Vivo Fluorescence Confocal Microscopy in the Assessment of Diagnostic Biopsies of the Prostate
title_full_unstemmed	Diagnostic Performance of Ex Vivo Fluorescence Confocal Microscopy in the Assessment of Diagnostic Biopsies of the Prostate
title_sort	diagnostic performance of ex vivo fluorescence confocal microscopy in the assessment of diagnostic biopsies of the prostate
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/78fcfef9fc2241e686c7f6b9a4cc9baa
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Diagnostic Performance of Ex Vivo Fluorescence Confocal Microscopy in the Assessment of Diagnostic Biopsies of the Prostate

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