Ultrarapid Measurement of Diagnostic Antibodies by Magnetic Capture of Immune Complexes

Abstract Rapid point-of-care, antibody-based testing is not currently available for the diagnosis of most autoimmune and infectious diseases. Here we report a simple, robust and ultrafast fluid-phase immunocapture method for clinical measurements of antibody levels. This method employs neodymium mag...

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Autores principales:	Peter D. Burbelo, Sreenivasulu Gunti, Jason M. Keller, Caryn G. Morse, Steven G. Deeks, Michail S. Lionakis, Amit Kapoor, Qingxue Li, Jeffrey I. Cohen, Abner L. Notkins, Ilias Alevizos
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/78ffd472316d43b7ba1ba63549685fb8
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spelling	oai:doaj.org-article:78ffd472316d43b7ba1ba63549685fb82021-12-02T15:06:18ZUltrarapid Measurement of Diagnostic Antibodies by Magnetic Capture of Immune Complexes10.1038/s41598-017-03786-72045-2322https://doaj.org/article/78ffd472316d43b7ba1ba63549685fb82017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03786-7https://doaj.org/toc/2045-2322Abstract Rapid point-of-care, antibody-based testing is not currently available for the diagnosis of most autoimmune and infectious diseases. Here we report a simple, robust and ultrafast fluid-phase immunocapture method for clinical measurements of antibody levels. This method employs neodymium magnetic sticks that capture protein A/G-coated paramagnetic beads bound to antibody-luciferase-labeled antigen complexes. We demonstrate the ability to effectively measure specific antibody levels in serum samples from patients with varied infectious or autoimmune disorders, and in the case of Sjögren’s syndrome directly in saliva, requiring about a minute per assay. We also show the feasibility of coupling this method with a hand-held luminometer for portable testing. Our method offers the potential to quickly diagnose a multitude of autoimmune and infectious diseases in point-of-care settings.Peter D. BurbeloSreenivasulu GuntiJason M. KellerCaryn G. MorseSteven G. DeeksMichail S. LionakisAmit KapoorQingxue LiJeffrey I. CohenAbner L. NotkinsIlias AlevizosNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Peter D. Burbelo Sreenivasulu Gunti Jason M. Keller Caryn G. Morse Steven G. Deeks Michail S. Lionakis Amit Kapoor Qingxue Li Jeffrey I. Cohen Abner L. Notkins Ilias Alevizos Ultrarapid Measurement of Diagnostic Antibodies by Magnetic Capture of Immune Complexes
description	Abstract Rapid point-of-care, antibody-based testing is not currently available for the diagnosis of most autoimmune and infectious diseases. Here we report a simple, robust and ultrafast fluid-phase immunocapture method for clinical measurements of antibody levels. This method employs neodymium magnetic sticks that capture protein A/G-coated paramagnetic beads bound to antibody-luciferase-labeled antigen complexes. We demonstrate the ability to effectively measure specific antibody levels in serum samples from patients with varied infectious or autoimmune disorders, and in the case of Sjögren’s syndrome directly in saliva, requiring about a minute per assay. We also show the feasibility of coupling this method with a hand-held luminometer for portable testing. Our method offers the potential to quickly diagnose a multitude of autoimmune and infectious diseases in point-of-care settings.
format	article
author	Peter D. Burbelo Sreenivasulu Gunti Jason M. Keller Caryn G. Morse Steven G. Deeks Michail S. Lionakis Amit Kapoor Qingxue Li Jeffrey I. Cohen Abner L. Notkins Ilias Alevizos
author_facet	Peter D. Burbelo Sreenivasulu Gunti Jason M. Keller Caryn G. Morse Steven G. Deeks Michail S. Lionakis Amit Kapoor Qingxue Li Jeffrey I. Cohen Abner L. Notkins Ilias Alevizos
author_sort	Peter D. Burbelo
title	Ultrarapid Measurement of Diagnostic Antibodies by Magnetic Capture of Immune Complexes
title_short	Ultrarapid Measurement of Diagnostic Antibodies by Magnetic Capture of Immune Complexes
title_full	Ultrarapid Measurement of Diagnostic Antibodies by Magnetic Capture of Immune Complexes
title_fullStr	Ultrarapid Measurement of Diagnostic Antibodies by Magnetic Capture of Immune Complexes
title_full_unstemmed	Ultrarapid Measurement of Diagnostic Antibodies by Magnetic Capture of Immune Complexes
title_sort	ultrarapid measurement of diagnostic antibodies by magnetic capture of immune complexes
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/78ffd472316d43b7ba1ba63549685fb8
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Ultrarapid Measurement of Diagnostic Antibodies by Magnetic Capture of Immune Complexes

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