Single Nucleotide Polymorphism of Insulin Receptor Gene rs2059806 in Polycystic Ovary Syndrome- A Case-control Study
Introduction: One of the most common endocrinopathies faced in clinical practice is Polycystic Ovary Syndrome (PCOS). Furthermore, it is one of the major causative factors of anovulatory infertility affecting a large number of female population worldwide. They are also at a greater risk for deve...
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| Autores principales: | , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
JCDR Research and Publications Private Limited
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/790aa4f84ad843bd8771ebf7458fb747 |
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| Sumario: | Introduction: One of the most common endocrinopathies faced in
clinical practice is Polycystic Ovary Syndrome (PCOS). Furthermore,
it is one of the major causative factors of anovulatory infertility
affecting a large number of female population worldwide. They are
also at a greater risk for developing impaired glucose tolerance,
type 2 diabetes mellitus and cardiovascular disease. Decreased
sensitivity to insulin is a common feature observed in women with
this syndrome.
Aim: To assess whether polymorphism of insulin receptor gene
has a significant role to develop the PCOS.
Materials and Methods: This was a case-control, observational,
hospital-based study, conducted at Department of Biochemistry,
Calcutta National Medical College, Kolkata from January 2018 to
September 2018. Total 123 patients with PCOS cases and 111
normo-ovulatory normal control female subjects were selected.
Blood samples were collected for estimation of serum testosterone
and Deoxyribonucleic Acid (DNA) extraction. Polymerase Chain
Reaction (PCR) followed by Restriction Fragment Length
Polymorphism (RFLP) pattern of PCR fragments of DNA samples
were determined. The study analysed and compared the genotyping
and allele frequencies of rs2059806 polymorphism in control and
case group. For comparison, chi-square test was performed
(Odds ratio, p<0.05).
Results: Amongst the 123 PCOS cases and 111 normal female
subjects of the study, mean age of PCOS patients were 22.59±4.7
years and that of control subjects was 21.9±5.1 years. The
present study observed an increase in mutant G allele in PCOS
subjects (Odds ratio-2.18, p=0.0035).
Conclusion: The polymorphism of insulin receptor gene rs2059806
may have a probable role in the development of PCOS. |
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