Single Nucleotide Polymorphism of Insulin Receptor Gene rs2059806 in Polycystic Ovary Syndrome- A Case-control Study

Introduction: One of the most common endocrinopathies faced in clinical practice is Polycystic Ovary Syndrome (PCOS). Furthermore, it is one of the major causative factors of anovulatory infertility affecting a large number of female population worldwide. They are also at a greater risk for deve...

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Autores principales:	Satwika Sinha, Kaushik Kar, Partha Pratim Chowdhury, Anindya Dasgupta
Formato:	article
Lenguaje:	EN
Publicado:	JCDR Research and Publications Private Limited 2021
Materias:	endocrinopathies genetic factors polymerase chain reaction restriction fragment length polymorphism testosterone Medicine R
Acceso en línea:	https://doaj.org/article/790aa4f84ad843bd8771ebf7458fb747
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spelling	oai:doaj.org-article:790aa4f84ad843bd8771ebf7458fb7472021-11-10T05:05:57ZSingle Nucleotide Polymorphism of Insulin Receptor Gene rs2059806 in Polycystic Ovary Syndrome- A Case-control Study10.7860/JCDR/2021/49653.153952249-782X0973-709Xhttps://doaj.org/article/790aa4f84ad843bd8771ebf7458fb7472021-09-01T00:00:00Zhttps://www.jcdr.net/articles/PDF/15395/49653_CE[Ra1]_F(SHU)_PF1(AKA_SHU)_PFA(AKA_KM)_PN(KM).pdfhttps://doaj.org/toc/2249-782Xhttps://doaj.org/toc/0973-709XIntroduction: One of the most common endocrinopathies faced in clinical practice is Polycystic Ovary Syndrome (PCOS). Furthermore, it is one of the major causative factors of anovulatory infertility affecting a large number of female population worldwide. They are also at a greater risk for developing impaired glucose tolerance, type 2 diabetes mellitus and cardiovascular disease. Decreased sensitivity to insulin is a common feature observed in women with this syndrome. Aim: To assess whether polymorphism of insulin receptor gene has a significant role to develop the PCOS. Materials and Methods: This was a case-control, observational, hospital-based study, conducted at Department of Biochemistry, Calcutta National Medical College, Kolkata from January 2018 to September 2018. Total 123 patients with PCOS cases and 111 normo-ovulatory normal control female subjects were selected. Blood samples were collected for estimation of serum testosterone and Deoxyribonucleic Acid (DNA) extraction. Polymerase Chain Reaction (PCR) followed by Restriction Fragment Length Polymorphism (RFLP) pattern of PCR fragments of DNA samples were determined. The study analysed and compared the genotyping and allele frequencies of rs2059806 polymorphism in control and case group. For comparison, chi-square test was performed (Odds ratio, p<0.05). Results: Amongst the 123 PCOS cases and 111 normal female subjects of the study, mean age of PCOS patients were 22.59±4.7 years and that of control subjects was 21.9±5.1 years. The present study observed an increase in mutant G allele in PCOS subjects (Odds ratio-2.18, p=0.0035). Conclusion: The polymorphism of insulin receptor gene rs2059806 may have a probable role in the development of PCOS.Satwika SinhaKaushik Kar Partha Pratim ChowdhuryAnindya DasguptaJCDR Research and Publications Private Limitedarticleendocrinopathiesgenetic factorspolymerase chain reactionrestriction fragment length polymorphismtestosteroneMedicineRENJournal of Clinical and Diagnostic Research, Vol 15, Iss 9, Pp GC07-GC10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	endocrinopathies genetic factors polymerase chain reaction restriction fragment length polymorphism testosterone Medicine R
spellingShingle	endocrinopathies genetic factors polymerase chain reaction restriction fragment length polymorphism testosterone Medicine R Satwika Sinha Kaushik Kar Partha Pratim Chowdhury Anindya Dasgupta Single Nucleotide Polymorphism of Insulin Receptor Gene rs2059806 in Polycystic Ovary Syndrome- A Case-control Study
description	Introduction: One of the most common endocrinopathies faced in clinical practice is Polycystic Ovary Syndrome (PCOS). Furthermore, it is one of the major causative factors of anovulatory infertility affecting a large number of female population worldwide. They are also at a greater risk for developing impaired glucose tolerance, type 2 diabetes mellitus and cardiovascular disease. Decreased sensitivity to insulin is a common feature observed in women with this syndrome. Aim: To assess whether polymorphism of insulin receptor gene has a significant role to develop the PCOS. Materials and Methods: This was a case-control, observational, hospital-based study, conducted at Department of Biochemistry, Calcutta National Medical College, Kolkata from January 2018 to September 2018. Total 123 patients with PCOS cases and 111 normo-ovulatory normal control female subjects were selected. Blood samples were collected for estimation of serum testosterone and Deoxyribonucleic Acid (DNA) extraction. Polymerase Chain Reaction (PCR) followed by Restriction Fragment Length Polymorphism (RFLP) pattern of PCR fragments of DNA samples were determined. The study analysed and compared the genotyping and allele frequencies of rs2059806 polymorphism in control and case group. For comparison, chi-square test was performed (Odds ratio, p<0.05). Results: Amongst the 123 PCOS cases and 111 normal female subjects of the study, mean age of PCOS patients were 22.59±4.7 years and that of control subjects was 21.9±5.1 years. The present study observed an increase in mutant G allele in PCOS subjects (Odds ratio-2.18, p=0.0035). Conclusion: The polymorphism of insulin receptor gene rs2059806 may have a probable role in the development of PCOS.
format	article
author	Satwika Sinha Kaushik Kar Partha Pratim Chowdhury Anindya Dasgupta
author_facet	Satwika Sinha Kaushik Kar Partha Pratim Chowdhury Anindya Dasgupta
author_sort	Satwika Sinha
title	Single Nucleotide Polymorphism of Insulin Receptor Gene rs2059806 in Polycystic Ovary Syndrome- A Case-control Study
title_short	Single Nucleotide Polymorphism of Insulin Receptor Gene rs2059806 in Polycystic Ovary Syndrome- A Case-control Study
title_full	Single Nucleotide Polymorphism of Insulin Receptor Gene rs2059806 in Polycystic Ovary Syndrome- A Case-control Study
title_fullStr	Single Nucleotide Polymorphism of Insulin Receptor Gene rs2059806 in Polycystic Ovary Syndrome- A Case-control Study
title_full_unstemmed	Single Nucleotide Polymorphism of Insulin Receptor Gene rs2059806 in Polycystic Ovary Syndrome- A Case-control Study
title_sort	single nucleotide polymorphism of insulin receptor gene rs2059806 in polycystic ovary syndrome- a case-control study
publisher	JCDR Research and Publications Private Limited
publishDate	2021
url	https://doaj.org/article/790aa4f84ad843bd8771ebf7458fb747
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Single Nucleotide Polymorphism of Insulin Receptor Gene rs2059806 in Polycystic Ovary Syndrome- A Case-control Study

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