Transcriptomic profile of tobacco in response to Phytophthora nicotianae infection

Abstract Black shank, caused by Phytophthora nicotianae (P. nicotianae), is a serious disease of cultivated tobacco (Nicotiana tabacum) worldwide. The interactions between tobacco and P. nicotianae are complex and the outcomes of the interactions depend on the tobacco genotype, P. nicotianae strain,...

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Autores principales:	Jian-Kang Yang, Zhi-Jun Tong, Dun-Huang Fang, Xue-Jun Chen, Ke-Qin Zhang, Bing-Guang Xiao
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/790fb22dd6634df6a478014b249ee10b
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spelling	oai:doaj.org-article:790fb22dd6634df6a478014b249ee10b2021-12-02T12:32:07ZTranscriptomic profile of tobacco in response to Phytophthora nicotianae infection10.1038/s41598-017-00481-52045-2322https://doaj.org/article/790fb22dd6634df6a478014b249ee10b2017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00481-5https://doaj.org/toc/2045-2322Abstract Black shank, caused by Phytophthora nicotianae (P. nicotianae), is a serious disease of cultivated tobacco (Nicotiana tabacum) worldwide. The interactions between tobacco and P. nicotianae are complex and the outcomes of the interactions depend on the tobacco genotype, P. nicotianae strain, and environmental conditions. In this study, we used RNA-sequencing (RNA-Seq) to investigate and compare transcriptional changes in the stems of tobacco upon inoculation with P. nicotianae strain race 0. We used two tobacco varieties: RBST (named from resistance to black shank and tobacco mosaic virus), which was resistant to the P. nicotianae strain race 0, and Honghuadajinyuan (HD), which was susceptible to P. nicotianae race 0. Samples were collected 12 and 72-hour post inoculation (hpi). Analysis of differentially expressed genes (DEGs) and significantly enriched GO terms indicated that several basic defense mechanisms were suppressed in both varieties, which included response to wounding (GO: 0009611), and defense response to fungus (GO: 0050832). We also found some genes that may especially be related to mechanisms of resistance in RBST, such as the one encoding a chitinase. These results will provide a valuable resource for understanding the interactions between P. nicotianae and tobacco plants.Jian-Kang YangZhi-Jun TongDun-Huang FangXue-Jun ChenKe-Qin ZhangBing-Guang XiaoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-7 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jian-Kang Yang Zhi-Jun Tong Dun-Huang Fang Xue-Jun Chen Ke-Qin Zhang Bing-Guang Xiao Transcriptomic profile of tobacco in response to Phytophthora nicotianae infection
description	Abstract Black shank, caused by Phytophthora nicotianae (P. nicotianae), is a serious disease of cultivated tobacco (Nicotiana tabacum) worldwide. The interactions between tobacco and P. nicotianae are complex and the outcomes of the interactions depend on the tobacco genotype, P. nicotianae strain, and environmental conditions. In this study, we used RNA-sequencing (RNA-Seq) to investigate and compare transcriptional changes in the stems of tobacco upon inoculation with P. nicotianae strain race 0. We used two tobacco varieties: RBST (named from resistance to black shank and tobacco mosaic virus), which was resistant to the P. nicotianae strain race 0, and Honghuadajinyuan (HD), which was susceptible to P. nicotianae race 0. Samples were collected 12 and 72-hour post inoculation (hpi). Analysis of differentially expressed genes (DEGs) and significantly enriched GO terms indicated that several basic defense mechanisms were suppressed in both varieties, which included response to wounding (GO: 0009611), and defense response to fungus (GO: 0050832). We also found some genes that may especially be related to mechanisms of resistance in RBST, such as the one encoding a chitinase. These results will provide a valuable resource for understanding the interactions between P. nicotianae and tobacco plants.
format	article
author	Jian-Kang Yang Zhi-Jun Tong Dun-Huang Fang Xue-Jun Chen Ke-Qin Zhang Bing-Guang Xiao
author_facet	Jian-Kang Yang Zhi-Jun Tong Dun-Huang Fang Xue-Jun Chen Ke-Qin Zhang Bing-Guang Xiao
author_sort	Jian-Kang Yang
title	Transcriptomic profile of tobacco in response to Phytophthora nicotianae infection
title_short	Transcriptomic profile of tobacco in response to Phytophthora nicotianae infection
title_full	Transcriptomic profile of tobacco in response to Phytophthora nicotianae infection
title_fullStr	Transcriptomic profile of tobacco in response to Phytophthora nicotianae infection
title_full_unstemmed	Transcriptomic profile of tobacco in response to Phytophthora nicotianae infection
title_sort	transcriptomic profile of tobacco in response to phytophthora nicotianae infection
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/790fb22dd6634df6a478014b249ee10b
work_keys_str_mv	AT jiankangyang transcriptomicprofileoftobaccoinresponsetophytophthoranicotianaeinfection AT zhijuntong transcriptomicprofileoftobaccoinresponsetophytophthoranicotianaeinfection AT dunhuangfang transcriptomicprofileoftobaccoinresponsetophytophthoranicotianaeinfection AT xuejunchen transcriptomicprofileoftobaccoinresponsetophytophthoranicotianaeinfection AT keqinzhang transcriptomicprofileoftobaccoinresponsetophytophthoranicotianaeinfection AT bingguangxiao transcriptomicprofileoftobaccoinresponsetophytophthoranicotianaeinfection
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Transcriptomic profile of tobacco in response to Phytophthora nicotianae infection

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