High-Resolution Morphological Approach to Analyse Elastic Laminae Injuries of the Ascending Aorta in a Murine Model of Marfan Syndrome

Abstract In Marfan syndrome, the tunica media is disrupted, which leads to the formation of ascending aortic aneurysms. Marfan aortic samples are histologically characterized by the fragmentation of elastic laminae. However, conventional histological techniques using transverse sections provide limi...

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Autores principales:	Júlia López-Guimet, Jordi Andilla, Pablo Loza-Alvarez, Gustavo Egea
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7910d0dc307d4d15880b3866975d6f2e
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spelling	oai:doaj.org-article:7910d0dc307d4d15880b3866975d6f2e2021-12-02T11:53:10ZHigh-Resolution Morphological Approach to Analyse Elastic Laminae Injuries of the Ascending Aorta in a Murine Model of Marfan Syndrome10.1038/s41598-017-01620-82045-2322https://doaj.org/article/7910d0dc307d4d15880b3866975d6f2e2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01620-8https://doaj.org/toc/2045-2322Abstract In Marfan syndrome, the tunica media is disrupted, which leads to the formation of ascending aortic aneurysms. Marfan aortic samples are histologically characterized by the fragmentation of elastic laminae. However, conventional histological techniques using transverse sections provide limited information about the precise location, progression and 3D extension of the microstructural changes that occur in each lamina. We implemented a method using multiphoton excitation fluorescence microscopy and computational image processing, which provides high-resolution en-face images of segmented individual laminae from unstained whole aortic samples. We showed that internal elastic laminae and successive 2nd laminae are injured to a different extent in murine Marfan aortae; in particular, the density and size of fenestrae changed. Moreover, microstructural injuries were concentrated in the aortic proximal and convex anatomical regions. Other parameters such as the waviness and thickness of each lamina remained unaltered. In conclusion, the method reported here is a useful, unique tool for en-face laminae microstructure assessment that can obtain quantitative three-dimensional information about vascular tissue. The application of this method to murine Marfan aortae clearly shows that the microstructural damage in elastic laminae is not equal throughout the thickness of the tunica media and in the different anatomical regions of the ascending aorta.Júlia López-GuimetJordi AndillaPablo Loza-AlvarezGustavo EgeaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Júlia López-Guimet Jordi Andilla Pablo Loza-Alvarez Gustavo Egea High-Resolution Morphological Approach to Analyse Elastic Laminae Injuries of the Ascending Aorta in a Murine Model of Marfan Syndrome
description	Abstract In Marfan syndrome, the tunica media is disrupted, which leads to the formation of ascending aortic aneurysms. Marfan aortic samples are histologically characterized by the fragmentation of elastic laminae. However, conventional histological techniques using transverse sections provide limited information about the precise location, progression and 3D extension of the microstructural changes that occur in each lamina. We implemented a method using multiphoton excitation fluorescence microscopy and computational image processing, which provides high-resolution en-face images of segmented individual laminae from unstained whole aortic samples. We showed that internal elastic laminae and successive 2nd laminae are injured to a different extent in murine Marfan aortae; in particular, the density and size of fenestrae changed. Moreover, microstructural injuries were concentrated in the aortic proximal and convex anatomical regions. Other parameters such as the waviness and thickness of each lamina remained unaltered. In conclusion, the method reported here is a useful, unique tool for en-face laminae microstructure assessment that can obtain quantitative three-dimensional information about vascular tissue. The application of this method to murine Marfan aortae clearly shows that the microstructural damage in elastic laminae is not equal throughout the thickness of the tunica media and in the different anatomical regions of the ascending aorta.
format	article
author	Júlia López-Guimet Jordi Andilla Pablo Loza-Alvarez Gustavo Egea
author_facet	Júlia López-Guimet Jordi Andilla Pablo Loza-Alvarez Gustavo Egea
author_sort	Júlia López-Guimet
title	High-Resolution Morphological Approach to Analyse Elastic Laminae Injuries of the Ascending Aorta in a Murine Model of Marfan Syndrome
title_short	High-Resolution Morphological Approach to Analyse Elastic Laminae Injuries of the Ascending Aorta in a Murine Model of Marfan Syndrome
title_full	High-Resolution Morphological Approach to Analyse Elastic Laminae Injuries of the Ascending Aorta in a Murine Model of Marfan Syndrome
title_fullStr	High-Resolution Morphological Approach to Analyse Elastic Laminae Injuries of the Ascending Aorta in a Murine Model of Marfan Syndrome
title_full_unstemmed	High-Resolution Morphological Approach to Analyse Elastic Laminae Injuries of the Ascending Aorta in a Murine Model of Marfan Syndrome
title_sort	high-resolution morphological approach to analyse elastic laminae injuries of the ascending aorta in a murine model of marfan syndrome
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/7910d0dc307d4d15880b3866975d6f2e
work_keys_str_mv	AT julialopezguimet highresolutionmorphologicalapproachtoanalyseelasticlaminaeinjuriesoftheascendingaortainamurinemodelofmarfansyndrome AT jordiandilla highresolutionmorphologicalapproachtoanalyseelasticlaminaeinjuriesoftheascendingaortainamurinemodelofmarfansyndrome AT pablolozaalvarez highresolutionmorphologicalapproachtoanalyseelasticlaminaeinjuriesoftheascendingaortainamurinemodelofmarfansyndrome AT gustavoegea highresolutionmorphologicalapproachtoanalyseelasticlaminaeinjuriesoftheascendingaortainamurinemodelofmarfansyndrome
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High-Resolution Morphological Approach to Analyse Elastic Laminae Injuries of the Ascending Aorta in a Murine Model of Marfan Syndrome

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