Extracellular vesicles from parasitic helminths contain specific excretory/secretory proteins and are internalized in intestinal host cells.

The study of host-parasite interactions has increased considerably in the last decades, with many studies focusing on the identification of parasite molecules (i.e. surface or excretory/secretory proteins (ESP)) as potential targets for new specific treatments and/or diagnostic tools. In parallel, i...

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Autores principales:	Antonio Marcilla, María Trelis, Alba Cortés, Javier Sotillo, Fernando Cantalapiedra, María Teresa Minguez, María Luz Valero, Manuel Mateo Sánchez del Pino, Carla Muñoz-Antoli, Rafael Toledo, Dolores Bernal
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7914f5d5a83f412c9f83ff5c79604471
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spelling	oai:doaj.org-article:7914f5d5a83f412c9f83ff5c796044712021-11-18T07:04:17ZExtracellular vesicles from parasitic helminths contain specific excretory/secretory proteins and are internalized in intestinal host cells.1932-620310.1371/journal.pone.0045974https://doaj.org/article/7914f5d5a83f412c9f83ff5c796044712012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23029346/?tool=EBIhttps://doaj.org/toc/1932-6203The study of host-parasite interactions has increased considerably in the last decades, with many studies focusing on the identification of parasite molecules (i.e. surface or excretory/secretory proteins (ESP)) as potential targets for new specific treatments and/or diagnostic tools. In parallel, in the last few years there have been significant advances in the field of extracellular vesicles research. Among these vesicles, exosomes of endocytic origin, with a characteristic size ranging from 30-100 nm, carry several atypical secreted proteins in different organisms, including parasitic protozoa. Here, we present experimental evidence for the existence of exosome-like vesicles in parasitic helminths, specifically the trematodes Echinostoma caproni and Fasciola hepatica. These microvesicles are actively released by the parasites and are taken up by host cells. Trematode extracellular vesicles contain most of the proteins previously identified as components of ESP, as confirmed by proteomic, immunogold labeling and electron microscopy studies. In addition to parasitic proteins, we also identify host proteins in these structures. The existence of extracellular vesicles explains the secretion of atypical proteins in trematodes, and the demonstration of their uptake by host cells suggests an important role for these structures in host-parasite communication, as described for other infectious agents.Antonio MarcillaMaría TrelisAlba CortésJavier SotilloFernando CantalapiedraMaría Teresa MinguezMaría Luz ValeroManuel Mateo Sánchez del PinoCarla Muñoz-AntoliRafael ToledoDolores BernalPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 9, p e45974 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Antonio Marcilla María Trelis Alba Cortés Javier Sotillo Fernando Cantalapiedra María Teresa Minguez María Luz Valero Manuel Mateo Sánchez del Pino Carla Muñoz-Antoli Rafael Toledo Dolores Bernal Extracellular vesicles from parasitic helminths contain specific excretory/secretory proteins and are internalized in intestinal host cells.
description	The study of host-parasite interactions has increased considerably in the last decades, with many studies focusing on the identification of parasite molecules (i.e. surface or excretory/secretory proteins (ESP)) as potential targets for new specific treatments and/or diagnostic tools. In parallel, in the last few years there have been significant advances in the field of extracellular vesicles research. Among these vesicles, exosomes of endocytic origin, with a characteristic size ranging from 30-100 nm, carry several atypical secreted proteins in different organisms, including parasitic protozoa. Here, we present experimental evidence for the existence of exosome-like vesicles in parasitic helminths, specifically the trematodes Echinostoma caproni and Fasciola hepatica. These microvesicles are actively released by the parasites and are taken up by host cells. Trematode extracellular vesicles contain most of the proteins previously identified as components of ESP, as confirmed by proteomic, immunogold labeling and electron microscopy studies. In addition to parasitic proteins, we also identify host proteins in these structures. The existence of extracellular vesicles explains the secretion of atypical proteins in trematodes, and the demonstration of their uptake by host cells suggests an important role for these structures in host-parasite communication, as described for other infectious agents.
format	article
author	Antonio Marcilla María Trelis Alba Cortés Javier Sotillo Fernando Cantalapiedra María Teresa Minguez María Luz Valero Manuel Mateo Sánchez del Pino Carla Muñoz-Antoli Rafael Toledo Dolores Bernal
author_facet	Antonio Marcilla María Trelis Alba Cortés Javier Sotillo Fernando Cantalapiedra María Teresa Minguez María Luz Valero Manuel Mateo Sánchez del Pino Carla Muñoz-Antoli Rafael Toledo Dolores Bernal
author_sort	Antonio Marcilla
title	Extracellular vesicles from parasitic helminths contain specific excretory/secretory proteins and are internalized in intestinal host cells.
title_short	Extracellular vesicles from parasitic helminths contain specific excretory/secretory proteins and are internalized in intestinal host cells.
title_full	Extracellular vesicles from parasitic helminths contain specific excretory/secretory proteins and are internalized in intestinal host cells.
title_fullStr	Extracellular vesicles from parasitic helminths contain specific excretory/secretory proteins and are internalized in intestinal host cells.
title_full_unstemmed	Extracellular vesicles from parasitic helminths contain specific excretory/secretory proteins and are internalized in intestinal host cells.
title_sort	extracellular vesicles from parasitic helminths contain specific excretory/secretory proteins and are internalized in intestinal host cells.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/7914f5d5a83f412c9f83ff5c79604471
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Extracellular vesicles from parasitic helminths contain specific excretory/secretory proteins and are internalized in intestinal host cells.

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