Detection of Secondary Metabolites as Biomarkers for the Early Diagnosis and Prevention of Type 2 Diabetes

Jumana Y Al-Aama,1,2 Hadiah B Al Mahdi,1 Mohammed A Salama,1 Khadija H Bakur,1,2 Amani Alhozali,3 Hala H Mosli,3 Suhad M Bahijri,4 Ahmed Bahieldin,5,6 Lothar Willmitzer,7 Sherif Edris1,5,6 1King Abdulaziz University, Princess Al Jawhara Albrahim Centre of Excellence in Research of Hereditary Disorde...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Al-Aama JY, Al Mahdi HB, Salama MA, Bakur KH, Alhozali A, Mosli HH, Bahijri SM, Bahieldin A, Willmitzer L, Edris S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	glucagon signaling glycine production bile secretion insulin sensitivity/resistance Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/79189e4935ce49fea4c9ae4666182588
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:79189e4935ce49fea4c9ae4666182588
record_format	dspace
spelling	oai:doaj.org-article:79189e4935ce49fea4c9ae46661825882021-12-02T04:48:38ZDetection of Secondary Metabolites as Biomarkers for the Early Diagnosis and Prevention of Type 2 Diabetes1178-7007https://doaj.org/article/79189e4935ce49fea4c9ae46661825882019-12-01T00:00:00Zhttps://www.dovepress.com/detection-of-secondary-metabolites-as-biomarkers-for-the-early-diagnos-peer-reviewed-article-DMSOhttps://doaj.org/toc/1178-7007Jumana Y Al-Aama,1,2 Hadiah B Al Mahdi,1 Mohammed A Salama,1 Khadija H Bakur,1,2 Amani Alhozali,3 Hala H Mosli,3 Suhad M Bahijri,4 Ahmed Bahieldin,5,6 Lothar Willmitzer,7 Sherif Edris1,5,6 1King Abdulaziz University, Princess Al Jawhara Albrahim Centre of Excellence in Research of Hereditary Disorders, Jeddah, KSA; 2King Abdulaziz University Faculty of Medicine, Department of Genetic Medicine, Jeddah, KSA; 3King Abdulaziz University, Faculty of Medicine, Department of Endocrinology and Metabolism, Jeddah, KSA; 4King Abdulaziz University, Faculty of Medicine, Department of Clinical Biochemistry, Jeddah, KSA; 5King Abdulaziz University, Faculty of Science, Biological Sciences Department, Jeddah, KSA; 6Ain Shams University, Department of Genetics, Cairo, Egypt; 7Max-Planck-Institut Für Molekulare Pflanzenphysiologie, Molecular Physiology, Golm, DE, GermanyCorrespondence: Sherif Edris; Jumana Y Al-AamaKing Abdulaziz University, Princess Al Jawhara Albrahim Centre of Excellence in Research of Hereditary Disorders, Jeddah, KSATel +966 593 66 23 84Email seedris@kau.edu.sa; jalama@kau.edu.saBackground: Type 2 diabetes, or T2D, is a metabolic disease that results in insulin resistance. In the present study, we hypothesize that metabolomic analysis in blood samples of T2D patients sharing the same ethnic background can recover new metabolic biomarkers and pathways that elucidate early diagnosis and predict the incidence of T2D.Methods: The study included 34 T2D patients and 33 healthy volunteers recruited between the years 2012 and 2013; the secondary metabolites were extracted from blood samples and analyzed using HPLC.Results: Principal coordinate analysis and hierarchical clustering patterns for the uncharacterized negatively and positively charged metabolites indicated that samples from healthy individuals and T2D patients were largely separated with only a few exceptions. The inspection of the top 10% secondary metabolites indicated an increase in fucose, tryptophan and choline levels in the T2D patients, while there was a reduction in carnitine, homoserine, allothreonine, serine and betaine as compared to healthy individuals. These metabolites participate mainly in three cross-talking pathways, namely “glucagon signaling”, “glycine, serine and threonine” and “bile secretion”. Reduced level of carnitine in T2D patients is known to participate in the impaired insulin-stimulated glucose utilization, while reduced betaine level in T2D patients is known as a common feature of this metabolic syndrome and can result in the reduced glycine production and the occurrence of insulin resistance. However, reduced levels of serine, homoserine and allothrionine, substrates for glycine production, indicate the depletion of glycine, thus possibly impair insulin sensitivity in T2D patients of the present study.Conclusion: We introduce serine, homoserine and allothrionine as new potential biomarkers of T2D.Keywords: glucagon signaling, glycine production, bile secretion, insulin sensitivity/resistanceAl-Aama JYAl Mahdi HBSalama MABakur KHAlhozali AMosli HHBahijri SMBahieldin AWillmitzer LEdris SDove Medical Pressarticleglucagon signalingglycine productionbile secretioninsulin sensitivity/resistanceSpecialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 12, Pp 2675-2684 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	glucagon signaling glycine production bile secretion insulin sensitivity/resistance Specialties of internal medicine RC581-951
spellingShingle	glucagon signaling glycine production bile secretion insulin sensitivity/resistance Specialties of internal medicine RC581-951 Al-Aama JY Al Mahdi HB Salama MA Bakur KH Alhozali A Mosli HH Bahijri SM Bahieldin A Willmitzer L Edris S Detection of Secondary Metabolites as Biomarkers for the Early Diagnosis and Prevention of Type 2 Diabetes
description	Jumana Y Al-Aama,1,2 Hadiah B Al Mahdi,1 Mohammed A Salama,1 Khadija H Bakur,1,2 Amani Alhozali,3 Hala H Mosli,3 Suhad M Bahijri,4 Ahmed Bahieldin,5,6 Lothar Willmitzer,7 Sherif Edris1,5,6 1King Abdulaziz University, Princess Al Jawhara Albrahim Centre of Excellence in Research of Hereditary Disorders, Jeddah, KSA; 2King Abdulaziz University Faculty of Medicine, Department of Genetic Medicine, Jeddah, KSA; 3King Abdulaziz University, Faculty of Medicine, Department of Endocrinology and Metabolism, Jeddah, KSA; 4King Abdulaziz University, Faculty of Medicine, Department of Clinical Biochemistry, Jeddah, KSA; 5King Abdulaziz University, Faculty of Science, Biological Sciences Department, Jeddah, KSA; 6Ain Shams University, Department of Genetics, Cairo, Egypt; 7Max-Planck-Institut Für Molekulare Pflanzenphysiologie, Molecular Physiology, Golm, DE, GermanyCorrespondence: Sherif Edris; Jumana Y Al-AamaKing Abdulaziz University, Princess Al Jawhara Albrahim Centre of Excellence in Research of Hereditary Disorders, Jeddah, KSATel +966 593 66 23 84Email seedris@kau.edu.sa; jalama@kau.edu.saBackground: Type 2 diabetes, or T2D, is a metabolic disease that results in insulin resistance. In the present study, we hypothesize that metabolomic analysis in blood samples of T2D patients sharing the same ethnic background can recover new metabolic biomarkers and pathways that elucidate early diagnosis and predict the incidence of T2D.Methods: The study included 34 T2D patients and 33 healthy volunteers recruited between the years 2012 and 2013; the secondary metabolites were extracted from blood samples and analyzed using HPLC.Results: Principal coordinate analysis and hierarchical clustering patterns for the uncharacterized negatively and positively charged metabolites indicated that samples from healthy individuals and T2D patients were largely separated with only a few exceptions. The inspection of the top 10% secondary metabolites indicated an increase in fucose, tryptophan and choline levels in the T2D patients, while there was a reduction in carnitine, homoserine, allothreonine, serine and betaine as compared to healthy individuals. These metabolites participate mainly in three cross-talking pathways, namely “glucagon signaling”, “glycine, serine and threonine” and “bile secretion”. Reduced level of carnitine in T2D patients is known to participate in the impaired insulin-stimulated glucose utilization, while reduced betaine level in T2D patients is known as a common feature of this metabolic syndrome and can result in the reduced glycine production and the occurrence of insulin resistance. However, reduced levels of serine, homoserine and allothrionine, substrates for glycine production, indicate the depletion of glycine, thus possibly impair insulin sensitivity in T2D patients of the present study.Conclusion: We introduce serine, homoserine and allothrionine as new potential biomarkers of T2D.Keywords: glucagon signaling, glycine production, bile secretion, insulin sensitivity/resistance
format	article
author	Al-Aama JY Al Mahdi HB Salama MA Bakur KH Alhozali A Mosli HH Bahijri SM Bahieldin A Willmitzer L Edris S
author_facet	Al-Aama JY Al Mahdi HB Salama MA Bakur KH Alhozali A Mosli HH Bahijri SM Bahieldin A Willmitzer L Edris S
author_sort	Al-Aama JY
title	Detection of Secondary Metabolites as Biomarkers for the Early Diagnosis and Prevention of Type 2 Diabetes
title_short	Detection of Secondary Metabolites as Biomarkers for the Early Diagnosis and Prevention of Type 2 Diabetes
title_full	Detection of Secondary Metabolites as Biomarkers for the Early Diagnosis and Prevention of Type 2 Diabetes
title_fullStr	Detection of Secondary Metabolites as Biomarkers for the Early Diagnosis and Prevention of Type 2 Diabetes
title_full_unstemmed	Detection of Secondary Metabolites as Biomarkers for the Early Diagnosis and Prevention of Type 2 Diabetes
title_sort	detection of secondary metabolites as biomarkers for the early diagnosis and prevention of type 2 diabetes
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/79189e4935ce49fea4c9ae4666182588
work_keys_str_mv	AT alaamajy detectionofsecondarymetabolitesasbiomarkersfortheearlydiagnosisandpreventionoftype2diabetes AT almahdihb detectionofsecondarymetabolitesasbiomarkersfortheearlydiagnosisandpreventionoftype2diabetes AT salamama detectionofsecondarymetabolitesasbiomarkersfortheearlydiagnosisandpreventionoftype2diabetes AT bakurkh detectionofsecondarymetabolitesasbiomarkersfortheearlydiagnosisandpreventionoftype2diabetes AT alhozalia detectionofsecondarymetabolitesasbiomarkersfortheearlydiagnosisandpreventionoftype2diabetes AT moslihh detectionofsecondarymetabolitesasbiomarkersfortheearlydiagnosisandpreventionoftype2diabetes AT bahijrism detectionofsecondarymetabolitesasbiomarkersfortheearlydiagnosisandpreventionoftype2diabetes AT bahieldina detectionofsecondarymetabolitesasbiomarkersfortheearlydiagnosisandpreventionoftype2diabetes AT willmitzerl detectionofsecondarymetabolitesasbiomarkersfortheearlydiagnosisandpreventionoftype2diabetes AT edriss detectionofsecondarymetabolitesasbiomarkersfortheearlydiagnosisandpreventionoftype2diabetes
_version_	1718401014590078976

Detection of Secondary Metabolites as Biomarkers for the Early Diagnosis and Prevention of Type 2 Diabetes

Ejemplares similares