Shugosin 2 is a biomarker for pathological grading and survival prediction in patients with gliomas

Abstract Glioblastomas are the most common type of adult primary brain neoplasms. Clinically, it is helpful to identify biomarkers to predict the survival of patients with gliomas due to its poor outcome. Shugoshin 2 (SGO2) is critical in cell division and cell cycle progression in eukaryotes. Howev...

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Autores principales:	Ying Kao, Wen-Chiuan Tsai, Ssu-Han Chen, Shao-Yuan Hsu, Li-Chun Huang, Chih-Ju Chang, Shih-Ming Huang, Dueng-Yuan Hueng
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/791bcc3a9edc45d181950cfb3b5d0ff0
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spelling	oai:doaj.org-article:791bcc3a9edc45d181950cfb3b5d0ff02021-12-02T17:25:33ZShugosin 2 is a biomarker for pathological grading and survival prediction in patients with gliomas10.1038/s41598-021-97119-42045-2322https://doaj.org/article/791bcc3a9edc45d181950cfb3b5d0ff02021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97119-4https://doaj.org/toc/2045-2322Abstract Glioblastomas are the most common type of adult primary brain neoplasms. Clinically, it is helpful to identify biomarkers to predict the survival of patients with gliomas due to its poor outcome. Shugoshin 2 (SGO2) is critical in cell division and cell cycle progression in eukaryotes. However, the association of SGO2 with pathological grading and survival in patients with gliomas remains unclear. We analyzed the association between SGO2 expression and clinical outcomes from Gene Expression Omnibus (GEO) dataset profiles, The Cancer Genome Atlas (TCGA), and Chinese Glioma Genome Atlas (CGGA). SGO2 mRNA and protein expression in normal brain tissue and glioma cell lines were investigated via quantitative RT-PCR, Western blot, and IHC staining. The roles of SGO2 in proliferation, migration, and apoptosis of GBM cells were studied with wound-healing assay, BrdU assay, cell cycle analysis, and JC-1 assay. The protein–protein interaction (PPI) was analyzed via Search Tool for the Retrieval of Interacting Genes/Proteins (STRING). SGO2 mRNA expression predicted higher grade gliomas than non-tumor brain tissues. Kaplan–Meier survival analysis showed that patients with high-grade gliomas with a higher SGO2 expression had worse survival outcomes. SGO2 mRNA and protein expression were upper regulated in gliomas than in normal brain tissue. Inhibition of SGO2 suppressed cell proliferation and migration. Also, PPI result showed SGO2 to be a potential hub protein, which was related to the expression of AURKB and FOXM1. SGO2 expression positively correlates with WHO pathological grading and patient survival, suggesting that SGO2 is a biomarker that is predictive of disease progression in patients with gliomas.Ying KaoWen-Chiuan TsaiSsu-Han ChenShao-Yuan HsuLi-Chun HuangChih-Ju ChangShih-Ming HuangDueng-Yuan HuengNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ying Kao Wen-Chiuan Tsai Ssu-Han Chen Shao-Yuan Hsu Li-Chun Huang Chih-Ju Chang Shih-Ming Huang Dueng-Yuan Hueng Shugosin 2 is a biomarker for pathological grading and survival prediction in patients with gliomas
description	Abstract Glioblastomas are the most common type of adult primary brain neoplasms. Clinically, it is helpful to identify biomarkers to predict the survival of patients with gliomas due to its poor outcome. Shugoshin 2 (SGO2) is critical in cell division and cell cycle progression in eukaryotes. However, the association of SGO2 with pathological grading and survival in patients with gliomas remains unclear. We analyzed the association between SGO2 expression and clinical outcomes from Gene Expression Omnibus (GEO) dataset profiles, The Cancer Genome Atlas (TCGA), and Chinese Glioma Genome Atlas (CGGA). SGO2 mRNA and protein expression in normal brain tissue and glioma cell lines were investigated via quantitative RT-PCR, Western blot, and IHC staining. The roles of SGO2 in proliferation, migration, and apoptosis of GBM cells were studied with wound-healing assay, BrdU assay, cell cycle analysis, and JC-1 assay. The protein–protein interaction (PPI) was analyzed via Search Tool for the Retrieval of Interacting Genes/Proteins (STRING). SGO2 mRNA expression predicted higher grade gliomas than non-tumor brain tissues. Kaplan–Meier survival analysis showed that patients with high-grade gliomas with a higher SGO2 expression had worse survival outcomes. SGO2 mRNA and protein expression were upper regulated in gliomas than in normal brain tissue. Inhibition of SGO2 suppressed cell proliferation and migration. Also, PPI result showed SGO2 to be a potential hub protein, which was related to the expression of AURKB and FOXM1. SGO2 expression positively correlates with WHO pathological grading and patient survival, suggesting that SGO2 is a biomarker that is predictive of disease progression in patients with gliomas.
format	article
author	Ying Kao Wen-Chiuan Tsai Ssu-Han Chen Shao-Yuan Hsu Li-Chun Huang Chih-Ju Chang Shih-Ming Huang Dueng-Yuan Hueng
author_facet	Ying Kao Wen-Chiuan Tsai Ssu-Han Chen Shao-Yuan Hsu Li-Chun Huang Chih-Ju Chang Shih-Ming Huang Dueng-Yuan Hueng
author_sort	Ying Kao
title	Shugosin 2 is a biomarker for pathological grading and survival prediction in patients with gliomas
title_short	Shugosin 2 is a biomarker for pathological grading and survival prediction in patients with gliomas
title_full	Shugosin 2 is a biomarker for pathological grading and survival prediction in patients with gliomas
title_fullStr	Shugosin 2 is a biomarker for pathological grading and survival prediction in patients with gliomas
title_full_unstemmed	Shugosin 2 is a biomarker for pathological grading and survival prediction in patients with gliomas
title_sort	shugosin 2 is a biomarker for pathological grading and survival prediction in patients with gliomas
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/791bcc3a9edc45d181950cfb3b5d0ff0
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_version_	1718380919091363840

Shugosin 2 is a biomarker for pathological grading and survival prediction in patients with gliomas

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