ApoE Influences the Blood-Brain Barrier Through the NF-κB/MMP-9 Pathway After Traumatic Brain Injury
Abstract Apolipoprotein E (ApoE), encoded by the ApoE gene (APOE), influences the outcomes of traumatic brain injury (TBI), but the mechanism remains unclear. The present study aimed to investigate the effects of different ApoEs on the outcome of TBI and to explore the possible mechanisms. Controlle...
Guardado en:
| Autores principales: | , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2017
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/791db166549c44bfb3bfe45c229d2e63 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:791db166549c44bfb3bfe45c229d2e63 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:791db166549c44bfb3bfe45c229d2e632021-12-02T12:30:51ZApoE Influences the Blood-Brain Barrier Through the NF-κB/MMP-9 Pathway After Traumatic Brain Injury10.1038/s41598-017-06932-32045-2322https://doaj.org/article/791db166549c44bfb3bfe45c229d2e632017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06932-3https://doaj.org/toc/2045-2322Abstract Apolipoprotein E (ApoE), encoded by the ApoE gene (APOE), influences the outcomes of traumatic brain injury (TBI), but the mechanism remains unclear. The present study aimed to investigate the effects of different ApoEs on the outcome of TBI and to explore the possible mechanisms. Controlled cortical impact (CCI) was performed on APOEε3 (E3) and APOEε4 (E4) transgenic mice, APOE-KO (KO) mice, and wild type (WT) mice to construct an in vivo TBI model. Neurological deficits, blood brain barrier (BBB) permeability and brain edema were detected at days 1, 3, and 7 after TBI. The results revealed no significant differences among the four groups at day 1 or day 3 after injury, but more severe deficits were found in E4 and KO mice than in E3 and WT mice. Furthermore, a significant loss of tight junction proteins was observed in E4 and KO mice compared with E3 and WT mice at day 7. Additionally, more expression and activation of NF-κB and MMP-9 were found in E4 mice compared with E3 mice. Different ApoEs had distinct effects on neuro-function and BBB integrity after TBI. ApoE3, but not E4, might inhibit the NF-κB/MMP-9 pathway to alleviate BBB disruption and improve TBI outcomes.Zhipeng TengZongduo GuoJianjun ZhongChongjie ChengZhijian HuangYue WuShuang TangChao LuoXing PengHaitao WuXiaochuan SunLi JiangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Zhipeng Teng Zongduo Guo Jianjun Zhong Chongjie Cheng Zhijian Huang Yue Wu Shuang Tang Chao Luo Xing Peng Haitao Wu Xiaochuan Sun Li Jiang ApoE Influences the Blood-Brain Barrier Through the NF-κB/MMP-9 Pathway After Traumatic Brain Injury |
| description |
Abstract Apolipoprotein E (ApoE), encoded by the ApoE gene (APOE), influences the outcomes of traumatic brain injury (TBI), but the mechanism remains unclear. The present study aimed to investigate the effects of different ApoEs on the outcome of TBI and to explore the possible mechanisms. Controlled cortical impact (CCI) was performed on APOEε3 (E3) and APOEε4 (E4) transgenic mice, APOE-KO (KO) mice, and wild type (WT) mice to construct an in vivo TBI model. Neurological deficits, blood brain barrier (BBB) permeability and brain edema were detected at days 1, 3, and 7 after TBI. The results revealed no significant differences among the four groups at day 1 or day 3 after injury, but more severe deficits were found in E4 and KO mice than in E3 and WT mice. Furthermore, a significant loss of tight junction proteins was observed in E4 and KO mice compared with E3 and WT mice at day 7. Additionally, more expression and activation of NF-κB and MMP-9 were found in E4 mice compared with E3 mice. Different ApoEs had distinct effects on neuro-function and BBB integrity after TBI. ApoE3, but not E4, might inhibit the NF-κB/MMP-9 pathway to alleviate BBB disruption and improve TBI outcomes. |
| format |
article |
| author |
Zhipeng Teng Zongduo Guo Jianjun Zhong Chongjie Cheng Zhijian Huang Yue Wu Shuang Tang Chao Luo Xing Peng Haitao Wu Xiaochuan Sun Li Jiang |
| author_facet |
Zhipeng Teng Zongduo Guo Jianjun Zhong Chongjie Cheng Zhijian Huang Yue Wu Shuang Tang Chao Luo Xing Peng Haitao Wu Xiaochuan Sun Li Jiang |
| author_sort |
Zhipeng Teng |
| title |
ApoE Influences the Blood-Brain Barrier Through the NF-κB/MMP-9 Pathway After Traumatic Brain Injury |
| title_short |
ApoE Influences the Blood-Brain Barrier Through the NF-κB/MMP-9 Pathway After Traumatic Brain Injury |
| title_full |
ApoE Influences the Blood-Brain Barrier Through the NF-κB/MMP-9 Pathway After Traumatic Brain Injury |
| title_fullStr |
ApoE Influences the Blood-Brain Barrier Through the NF-κB/MMP-9 Pathway After Traumatic Brain Injury |
| title_full_unstemmed |
ApoE Influences the Blood-Brain Barrier Through the NF-κB/MMP-9 Pathway After Traumatic Brain Injury |
| title_sort |
apoe influences the blood-brain barrier through the nf-κb/mmp-9 pathway after traumatic brain injury |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/791db166549c44bfb3bfe45c229d2e63 |
| work_keys_str_mv |
AT zhipengteng apoeinfluencesthebloodbrainbarrierthroughthenfkbmmp9pathwayaftertraumaticbraininjury AT zongduoguo apoeinfluencesthebloodbrainbarrierthroughthenfkbmmp9pathwayaftertraumaticbraininjury AT jianjunzhong apoeinfluencesthebloodbrainbarrierthroughthenfkbmmp9pathwayaftertraumaticbraininjury AT chongjiecheng apoeinfluencesthebloodbrainbarrierthroughthenfkbmmp9pathwayaftertraumaticbraininjury AT zhijianhuang apoeinfluencesthebloodbrainbarrierthroughthenfkbmmp9pathwayaftertraumaticbraininjury AT yuewu apoeinfluencesthebloodbrainbarrierthroughthenfkbmmp9pathwayaftertraumaticbraininjury AT shuangtang apoeinfluencesthebloodbrainbarrierthroughthenfkbmmp9pathwayaftertraumaticbraininjury AT chaoluo apoeinfluencesthebloodbrainbarrierthroughthenfkbmmp9pathwayaftertraumaticbraininjury AT xingpeng apoeinfluencesthebloodbrainbarrierthroughthenfkbmmp9pathwayaftertraumaticbraininjury AT haitaowu apoeinfluencesthebloodbrainbarrierthroughthenfkbmmp9pathwayaftertraumaticbraininjury AT xiaochuansun apoeinfluencesthebloodbrainbarrierthroughthenfkbmmp9pathwayaftertraumaticbraininjury AT lijiang apoeinfluencesthebloodbrainbarrierthroughthenfkbmmp9pathwayaftertraumaticbraininjury |
| _version_ |
1718394286803779584 |